Agilent 5982-9120 20-Position Manual Vacuum Manifold for Solid Phase Extraction

Overview

The Agilent 5982-9120 20-Position Manual Vacuum Manifold is a precision-engineered platform for reproducible, high-throughput solid phase extraction (SPE) in regulated and research laboratory environments. Designed around the fundamental principles of vacuum-assisted column elution, this manifold enables controlled, gravity-independent sample loading, washing, and elution across 20 parallel SPE columns—ideal for method development, QC batch processing, and multi-analyte sample preparation prior to GC, LC, or LC-MS analysis. Its modular architecture integrates seamlessly with Agilent SampliQ SPE cartridges (e.g., C18, SCX, Florisil, and mixed-mode phases), ensuring consistent bed packing integrity and minimal channel-to-channel variability. Unlike automated systems, this manual manifold provides full operator control over vacuum pressure and flow kinetics—critical for optimizing retention, selectivity, and recovery of analytes with diverse polarities and molecular weights.

Key Features

• Borosilicate glass vacuum chamber with chemical resistance to common organic solvents (e.g., methanol, acetonitrile, dichloromethane, ethyl acetate) and aqueous buffers—rated for sustained operation under ≤25 inHg (≈635 mbar) vacuum.
• Individually adjustable PTFE-tipped pistons per port, enabling precise, independent flow regulation for each of the 20 SPE columns—eliminating cross-channel flow imbalance and ensuring uniform breakthrough behavior.
• Dual-scale vacuum gauge with coarse (0–30 inHg) and fine (0–5 inHg) adjustment dials, coupled with a stainless steel safety quick-release valve compliant with ISO 7241-1 (vacuum system safety standards).
• Height-adjustable universal rack support accommodates collection tubes ranging from 13 × 75 mm to 16 × 100 mm—compatible with standard 12 mL, 15 mL, and 50 mL polypropylene or glass vials.
• UHMW polyethylene base and sealing components provide long-term solvent compatibility with aggressive media including 0.1 M NaOH, 10% HCl, and 100% THF—validated per ASTM D4000 classification for polymer chemical resistance.
• Luer-Lock compatible port design ensures secure, leak-free connection to all standard 1/4″–28 threaded SPE cartridges and filtration devices without adapters.
• High-contrast laser-etched port numbering and rack alignment markers reduce operator error during multi-step protocols—supporting GLP-aligned documentation practices.

Sample Compatibility & Compliance

The manifold supports all commercially available 1 mL, 3 mL, 6 mL, and 10 mL SPE cartridges with Luer-Lock or Luer-Slip fittings—including Agilent SampliQ, Waters Sep-Pak, Thermo HyperSep, and generic silica- or polymer-based sorbents. It accommodates sequential solvent delivery using up to five distinct eluents via optional solvent selector valves (not included). The borosilicate glass construction meets USP requirements for inertness in pharmaceutical sample prep, while its mechanical design aligns with ISO/IEC 17025:2017 clause 6.4.2 (equipment suitability verification). No electrical components are present; therefore, no CE or UL certification is required—simplifying installation in Class I Div 1 fume hoods per NFPA 45.

Software & Data Management

As a fully manual, non-electronic instrument, the 5982-9120 does not incorporate embedded firmware, onboard memory, or digital interfaces. All operational parameters—including vacuum setpoint, dwell time per step, and solvent sequence—are documented externally via laboratory notebooks or electronic lab notebooks (ELNs) such as LabArchives or Benchling. The device supports audit-trail-ready workflows when used in conjunction with Agilent OpenLab CDS or Empower software for downstream chromatographic analysis, fulfilling FDA 21 CFR Part 11 requirements for traceability of sample preparation conditions.

Applications

• Environmental analysis: EPA Methods 508.1, 525.3, and 8270D sample cleanup for pesticides, PAHs, and chlorinated hydrocarbons in water and soil extracts.
• Pharmaceutical QC: Residual solvent removal and active pharmaceutical ingredient (API) purification per ICH Q2(R2) validation guidelines.
• Clinical toxicology: Plasma and urine deproteinization and analyte enrichment for opioids, benzodiazepines, and stimulants prior to LC-MS/MS quantitation.
• Food safety testing: Mycotoxin isolation (e.g., aflatoxins, ochratoxin A) from cereal matrices per AOAC 2005.02 and EU Commission Regulation (EC) No 401/2006.
• Academic research: Method scouting for novel sorbent chemistries, including molecularly imprinted polymers (MIPs) and metal-organic frameworks (MOFs), where flow-rate sensitivity directly impacts binding efficiency.

FAQ

Is the 5982-9120 compatible with non-Agilent SPE cartridges?
Yes—it accepts any Luer-Lock or Luer-Slip SPE cartridge with standard dimensions (e.g., 13 mm OD, 100 mm length), including third-party and custom-packed columns.
What vacuum source is recommended?
A diaphragm pump capable of sustaining ≥25 inHg vacuum (e.g., Agilent G1201-60100 or KNF N840.3T) with integrated oil mist filter is advised for long-term stability and solvent vapor management.
Can the manifold be sterilized for microbiological applications?
No—the glass chamber and PTFE components are not autoclavable; however, they can be cleaned with ethanol, isopropanol, or dilute sodium hypochlorite followed by thorough air drying.
Are replacement parts traceable to lot numbers?
Yes—each component (e.g., 5982-9121, 5982-9123) carries a unique Agilent part number and is supplied with a Certificate of Conformance referencing ISO 9001:2015 manufacturing controls.
Does Agilent offer validation documentation for this manifold?
While no IQ/OQ/PQ protocol is shipped with the unit, Agilent provides a User Verification Guide (P/N 5991-0105EN) outlining performance checks for vacuum integrity, flow uniformity, and dimensional compliance—suitable for internal qualification.