Animal Automatic Blood Sampling System ABS2
| Origin | USA |
|---|---|
| Manufacturer Type | Distributor |
| Origin Category | Imported |
| Model | ABS2 |
| Pricing | Upon Request |
Overview
The Animal Automatic Blood Sampling System ABS2 is an engineered platform designed for precise, repeatable, and minimally invasive serial blood collection from conscious or lightly restrained laboratory animals—primarily rodents (mice, rats) and small mammals. Unlike manual tail-vein or retro-orbital sampling methods, the ABS2 integrates programmable peristaltic fluid handling, real-time pressure monitoring, and closed-loop volume control to automate the entire blood withdrawal, anticoagulant mixing, and sample aliquoting workflow. It operates on the principle of controlled negative pressure aspiration combined with dynamic flow regulation, ensuring consistent draw rates (typically 10–100 µL/min), minimal hemolysis, and physiological stability during repeated sampling intervals (e.g., every 15–60 min over 24–72 h). The system is intended for use in pharmacokinetic (PK), toxicokinetic (TK), and circadian biomarker studies where temporal resolution and animal welfare compliance are critical.
Key Features
- Automated, software-controlled blood withdrawal with adjustable draw volume (10–500 µL per event), interval timing (1–360 min), and maximum session duration (up to 96 h)
- Integrated dual-channel peristaltic pump with calibrated tubing sets for simultaneous heparinized saline priming and sample transfer
- Real-time pressure feedback circuitry to detect catheter occlusion, clot formation, or vessel collapse—triggering automatic pause and alert
- Modular, sterilizable fluid path using USP Class VI-certified silicone and polyetheretherketone (PEEK) components
- Compatible with standard 22–27G indwelling catheters (jugular, carotid, or femoral) and pre-connected vascular access buttons (VABs)
- Compact benchtop footprint (32 × 24 × 20 cm) with CE-marked power supply and IEC 61000-6-3 EMC compliance
Sample Compatibility & Compliance
The ABS2 supports whole blood, plasma, and serum collection across species including C57BL/6 and CD-1 mice, Sprague-Dawley and Wistar rats, and New Zealand White rabbits. All wetted materials comply with ISO 10993-5 (cytotoxicity) and ISO 10993-10 (irritation/sensitization) requirements. The system architecture adheres to Good Laboratory Practice (GLP) documentation standards, enabling full audit trails—including operator ID, timestamped sampling events, volume logs, and error codes—for regulatory submissions (FDA IND/IDE, EMA CTIS). Optional 21 CFR Part 11-compliant software module provides electronic signatures, role-based access control, and immutable data archiving.
Software & Data Management
The ABS2 is operated via Windows-based ControlSuite v3.2 software, featuring a validated graphical interface with intuitive protocol builder, live flow-rate visualization, and automated CSV export. Data files include metadata headers (animal ID, study number, catheter type, ambient temperature), raw pressure/time traces, and calculated hematocrit-adjusted volume corrections. Export formats support direct ingestion into Phoenix WinNonlin, SAS, and R for PK/PD modeling. Audit logs record all configuration changes, user logins, and emergency stop activations—retained for ≥36 months per GLP retention guidelines.
Applications
- Chronopharmacokinetic profiling under undisturbed conditions (e.g., oral drug absorption across light/dark cycles)
- Repeated biomarker sampling in sepsis, diabetes, or inflammation models without stress-induced cortisol interference
- Microdosing studies requiring <50 µL serial samples for LC-MS/MS analysis
- Validation of implantable biosensors by correlating interstitial fluid analyte trends with concurrent arterial blood references
- Refinement of 3R-compliant protocols in accordance with NIH OLAW and EU Directive 2010/63/EU Annex VIII
FAQ
Is the ABS2 compatible with telemetry-integrated studies?
Yes—the system includes TTL-level synchronization inputs to align blood draw triggers with implanted telemetry device recordings (e.g., DSI TA-F10, Data Sciences International).
Can it be used for neonatal or pediatric rodent models?
With optional micro-catheter kits (28G PTFE, 0.05 mm ID), the ABS2 supports sampling from postnatal day 7–14 pups; minimum recommended blood volume per draw is 5 µL.
Does the system require external gas supply or vacuum source?
No—all aspiration and flush functions are self-contained via integrated brushless DC pumps; no compressed air or central vacuum connection is needed.
How is calibration performed and how often is it required?
Factory calibration is traceable to NIST standards; field verification uses gravimetric test kits (included) every 30 days or after tubing replacement—procedure documented in SOP-ABS2-CAL-01.
