BIO-DL GelDoc Pro Automated Gel Imaging System
| Brand | BIO-DL |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | GelDoc Pro |
| Pricing | Available Upon Request |
Overview
The BIO-DL GelDoc Pro Automated Gel Imaging System is a fully integrated, benchtop-grade gel documentation platform engineered for precise visualization, capture, and quantitative analysis of nucleic acid and protein gels. It operates on the principle of UV transillumination (254 nm and 302 nm standard wavelengths) combined with high-sensitivity monochrome CCD imaging under controlled darkroom conditions. The system supports ethidium bromide (EtBr), SYBR® Safe, GelRed®, Coomassie Blue, and silver-stained gels—enabling reliable detection across agarose, polyacrylamide (PAGE), and SDS-PAGE formats. Designed for routine molecular biology laboratories, QC facilities, and academic core facilities, the GelDoc Pro delivers consistent image acquisition with minimal user intervention, reducing inter-operator variability and supporting reproducible downstream quantitation in compliance with GLP-aligned workflows.
Key Features
- Fully automated imaging workflow: One-touch acquisition with preconfigured protocols for common stains (DNA/RNA, proteins), eliminating manual exposure time optimization.
- High-resolution monochrome CCD sensor (≥16-bit dynamic range, 2.8-megapixel effective resolution) optimized for low-light fluorescence and chemiluminescence detection.
- Dual-wavelength UV transilluminator (254 nm for DNA crosslinking and high-sensitivity detection; 302 nm for reduced photodamage during routine visualization).
- Motorized zoom lens with fixed f/1.2 aperture ensures consistent focus and uniform illumination across variable gel sizes (up to 25 × 25 cm).
- Integrated white-light reflective illumination module for documentation of stained protein gels, blots, and petri dishes without UV exposure.
- Robust aluminum-alloy chassis with light-tight enclosure and UV-blocking safety interlock door switch compliant with IEC 61010-1 safety standards.
Sample Compatibility & Compliance
The GelDoc Pro accommodates standard electrophoresis formats including mini-gels (e.g., 8 × 10 cm), midi-gels (e.g., 16 × 16 cm), and full-size slab gels (up to 25 × 25 cm). It supports both vertical and horizontal electrophoresis units, Western/Northern/Southern blot membranes, and colony plates. All optical components are calibrated per ISO 9001 manufacturing protocols. Image metadata—including exposure time, lens aperture, UV intensity, and timestamp—is embedded in TIFF and PNG exports to support audit trails required under FDA 21 CFR Part 11–aligned data integrity practices. System validation documentation (IQ/OQ templates) is provided for GxP-regulated environments.
Software & Data Management
The proprietary GelAnalyzer Pro software (v5.2+) runs on Windows 10/11 and provides intuitive, guided workflows from image capture to publication-ready output. Key capabilities include background subtraction, lane/band detection using adaptive thresholding, molecular weight estimation via reference ladder fitting (linear or cubic spline interpolation), and relative quantitation (normalized band intensity vs. control or loading standard). Export options include CSV (for statistical analysis in R or Python), annotated TIFF/PNG, and PDF reports with embedded metadata. Audit log functionality records all user actions (e.g., intensity adjustments, band reselections), satisfying traceability requirements for ISO/IEC 17025-accredited labs.
Applications
- Quantitative analysis of PCR products, restriction digests, and plasmid preparations.
- Density-based normalization of protein expression in SDS-PAGE and Western blots.
- Verification of CRISPR editing efficiency via T7E1 or Surveyor assays.
- Documentation and archiving of electrophoretic results for regulatory submissions (e.g., IND, BLA dossiers).
- Teaching laboratory use: Preloaded templates for undergraduate molecular biology curricula align with AAAS Vision and Change competencies.
FAQ
Does the GelDoc Pro support chemiluminescent detection (e.g., ECL)?
Yes—via optional long-exposure mode (up to 30 minutes) and cooled CCD configuration (available as upgrade kit), enabling sensitive detection of horseradish peroxidase (HRP)- and alkaline phosphatase (AP)-based signals.
Can the system be integrated into a LIMS environment?
Yes—GelAnalyzer Pro supports HL7 and ASTM E1384-compliant data export; custom API integration is available upon request for enterprise-level LIMS connectivity.
Is UV exposure dose monitored and logged per sample?
Yes—the system records cumulative UV exposure (mJ/cm²) for each acquisition cycle and includes dose calibration certificates traceable to NIST standards.
What is the recommended maintenance schedule?
Annual optical alignment verification and CCD dark-current calibration are advised; UV lamp lifetime is rated at ≥5,000 hours with automatic usage logging in firmware.
Does the software comply with 21 CFR Part 11 requirements?
Out-of-the-box, it supports electronic signatures, role-based access control, and immutable audit logs—configuration assistance for full Part 11 compliance is included with enterprise licensing.
