BOXUN BSP-400 Biochemical Incubator

Overview

The BOXUN BSP-400 Biochemical Incubator is a precision-engineered, microprocessor-controlled environmental chamber designed for stable, long-term cultivation of bacteria, fungi, yeasts, and other aerobic or facultative anaerobic microorganisms under tightly regulated thermal conditions. Operating on a forced-air convection principle with an integrated high-efficiency refrigeration system, the BSP-400 maintains temperature stability across its full operating range of 0 °C to 60 °C—enabling both low-temperature preservation and elevated-temperature growth protocols. Its dual-mode capability supports critical applications including BOD (Biochemical Oxygen Demand) testing, microbial viability assays, antibiotic susceptibility studies, and plant tissue culture incubation. The unit complies with fundamental design principles outlined in ISO 13408-1 (aseptic processing environments), ASTM E171 (standard specifications for environmental chambers), and aligns with GLP-compliant laboratory infrastructure requirements for traceable, reproducible incubation conditions.

Key Features

• Intelligent microprocessor control system with backlit LCD display showing real-time temperature, setpoint, elapsed time, and programmable cycle status
• Proprietary airflow architecture utilizing a centrifugal blower and multi-channel internal ductwork to achieve uniform temperature distribution (±1 °C at 37 °C) without hot/cold zones
• Independent fresh-air inlet module with manual on/off valve—enabling controlled gas exchange while preserving chamber integrity during extended runs
• Integrated refrigeration system featuring an internationally certified hermetic compressor and ozone-safe R134a or R600a refrigerant, meeting EU F-Gas Regulation and China’s GB/T 7725 energy efficiency standards
• Energy-free automatic defrost technology that eliminates thermal disturbance during defrost cycles—ensuring uninterrupted incubation continuity
• Robust construction: cold-rolled steel outer shell with electrostatic epoxy-polyester coating; mirror-finish 304 stainless steel interior chamber with four height-adjustable shelves (load capacity: 15 kg per shelf)
• Comprehensive safety suite including over-temperature cut-off, door-open alarm, power-failure data retention, and auto-restart function with parameter recovery
• RS-485 serial interface compliant with Modbus RTU protocol for integration into centralized lab management systems or third-party data loggers

Sample Compatibility & Compliance

The BSP-400 accommodates standard microbiological containers—including Petri dishes (up to 150 mm), test tubes (16–25 mm OD), flasks (up to 2 L), and multi-well plates—within its 400 L usable volume. Its sealed chamber design (with optional inert gas purge compatibility via 30/60 mm external port fittings) supports anaerobic pre-conditioning when used with auxiliary gas regulators. The unit meets electrical safety requirements per GB 4793.1 (equivalent to IEC 61010-1) and electromagnetic compatibility per GB/T 18268.1. While not classified as a medical device, its performance consistency satisfies QC/QA validation criteria referenced in USP , ISO/IEC 17025, and FDA 21 CFR Part 11 when paired with audit-trail-enabled software extensions.

Software & Data Management

The embedded controller stores up to 10 user-defined programs, each comprising 99 repeatable segments with independent temperature, dwell time, and ramp rate settings. All operational parameters—including setpoints, alarms, and runtime logs—are retained in non-volatile memory during power interruption. When connected via RS-485 to a host PC running BOXUN’s optional LabLink™ software (Windows-compatible), users gain access to real-time graphing, exportable CSV reports, electronic signature support, and configurable alarm notifications (email/SMS via GPRS add-on). The system supports 21 CFR Part 11-compliant audit trails when deployed with validated software configurations and role-based user authentication.

Applications

• Biochemical Oxygen Demand (BOD₅) analysis per APHA Standard Methods 5210B and ISO 5815-1
• Microbial enumeration (e.g., total viable count, coliform detection) in environmental water, food, and pharmaceutical samples
• Fungal culture maintenance and sporulation studies requiring precise humidity-adjacent thermal control
• Seed germination and plant callus induction experiments under sterile, temperature-stabilized conditions
• Stability testing of diagnostic reagents and vaccine intermediates within ICH Q1A(R2)-aligned temperature profiles
• QC incubation for media sterility verification and growth promotion testing (USP , EP 2.6.12)

FAQ

What is the temperature recovery time after door opening?
Typically ≤12 minutes to return to ±0.5 °C of setpoint at 37 °C, verified per ASTM E742.
Can the BSP-400 operate continuously for 30 days or longer?
Yes—its self-diagnostic cooling system, low-energy defrost cycle, and thermal overload protection enable unattended 7×24 operation under nominal load conditions.
Is calibration certificate included with shipment?
A factory-issued calibration report (traceable to NIM, China) is provided; ISO/IEC 17025-accredited third-party calibration is available upon request.
Does the unit support CO₂ or humidity control?
No—this is a temperature-only incubator. For CO₂ or humidity integration, consider BOXUN’s BC series or partner with a dedicated humidification module vendor.
How is firmware updated?
Firmware upgrades are performed via RS-485 using BOXUN’s authorized service utility; no field-upgradable bootloader is exposed to end users for regulatory compliance integrity.