Chu Ding UV755B Ultraviolet-Visible Spectrophotometer

Overview

The Chu Ding UV755B Ultraviolet-Visible Spectrophotometer is a microprocessor-controlled, single-beam scanning instrument engineered for routine quantitative analysis across the ultraviolet, visible, and near-infrared spectral range (190–1100 nm). It operates on the fundamental principle of Beer–Lambert law-based absorbance measurement, utilizing a high-efficiency 1200 lines/mm holographic grating and precision optical components—including imported photodetectors—to deliver consistent photometric performance in laboratory and industrial settings. Designed for reliability under sustained operation, the UV755B features automatic wavelength calibration, dual-source management (deuterium lamp for UV, tungsten-halogen lamp for visible/NIR), and real-time lamp usage monitoring—ensuring traceable operational history and predictable maintenance scheduling. Its robust mechanical architecture and thermally stable optical bench support reproducible measurements essential for method validation and regulatory compliance.

Key Features

• Integrated 128 × 64 dot-matrix LCD display enabling direct visualization of absorbance spectra, standard curves, and numerical results without external software
• Onboard data storage capacity for up to 200 sample measurements and 100 user-named calibration curves—each independently retrievable via intuitive menu navigation
• USB 2.0 interface compliant with standard HID protocols, supporting seamless data export to external PCs and interoperability with YOKE 3.0 spectroscopic analysis software
• Automated source switching and lamp power control: deuterium and tungsten lamps are engaged or deactivated based on selected wavelength range, minimizing unnecessary thermal load and extending lamp service life
• Optimized sample compartment with adjustable cuvette holder accommodating standard pathlengths from 5 mm to 100 mm—including rectangular quartz, glass, and plastic cells
• Self-calibrating wavelength positioning system with motorized drive and encoder feedback, achieving ±1 nm accuracy and 0.5 nm repeatability across the full spectral range
• Front-panel membrane keypad with tactile feedback and logical layout, reducing operator training time and minimizing input errors in high-throughput environments

Sample Compatibility & Compliance

The UV755B accepts standard spectrophotometric sample formats including 10 mm square quartz cuvettes for UV applications (190–350 nm), borosilicate glass cells for visible-range work (350–1100 nm), and disposable plastic cuvettes for non-critical assays. Its 2 nm spectral bandwidth meets the resolution requirements specified in ISO 6425:2020 for general-purpose UV-Vis instrumentation and supports methodology aligned with ASTM E275, USP , and EP 2.2.25. While not pre-certified for 21 CFR Part 11, the instrument’s audit-ready features—including timestamped measurement logs, user-defined curve naming, and lamp runtime tracking—facilitate integration into GLP/GMP workflows when paired with validated software and procedural controls.

Software & Data Management

YOKE 3.0 software (optional) provides extended functionality including kinetic scan acquisition, multi-wavelength quantitation, derivative spectroscopy, and customizable report generation with embedded metadata (operator ID, date/time, instrument serial number, lamp hours). Raw absorbance data is exported in CSV format compatible with Excel, MATLAB, and Python-based analytical pipelines. The onboard firmware supports firmware updates via USB mass storage mode, ensuring long-term adaptability to evolving laboratory informatics standards. All stored calibration curves retain their original naming convention and slope/intercept parameters, enabling retrospective reprocessing without loss of traceability.

Applications

The UV755B serves as a foundational tool in quality control laboratories for pharmaceutical raw material identification (e.g., assay of paracetamol per USP monograph), food additive quantification (e.g., nitrate/nitrite determination in cured meats), environmental water testing (e.g., COD and phosphate analysis per APHA Standard Methods), clinical biochemistry (e.g., total protein via biuret assay), and academic research in physical chemistry (e.g., ligand-binding kinetics, acid dissociation constant determination). Its broad spectral coverage also supports pigment analysis in coatings, dye concentration monitoring in textile effluents, and semiconductor thin-film thickness estimation via interference fringe analysis.

FAQ

What wavelength range does the UV755B cover, and how is source switching managed?
The instrument operates from 190 nm to 1100 nm. A microcontroller automatically selects between the deuterium and tungsten-halogen lamps based on the set wavelength, with seamless transition at ~350 nm.
Is the UV755B suitable for GMP-regulated environments?
It supports key elements of GMP data integrity—such as user-assignable curve names, time-stamped measurements, and lamp usage logging—but requires complementary software validation and procedural documentation to meet full 21 CFR Part 11 requirements.
Can the instrument perform kinetic measurements?
Yes—when connected to YOKE 3.0 software, it supports time-scan mode with configurable interval timing (100 ms to 60 s per point) and up to 10,000 data points per run.
What is the maximum cuvette pathlength supported?
The sample chamber accommodates cuvettes up to 100 mm in optical pathlength, enabling low-concentration analysis without signal dilution.
Does the UV755B include wavelength calibration verification tools?
Yes—the firmware includes built-in holmium oxide and didymium filter validation routines, and users may perform manual peak checks at certified reference wavelengths (e.g., 253.7 nm, 365.0 nm, 546.1 nm).