DS JY-202A Rotary Microtome with Integrated Cryo Module

Overview

The DS JY-202A Rotary Microtome with Integrated Cryo Module is a dual-mode histological sectioning instrument engineered for precision and operational flexibility in both room-temperature paraffin embedding workflows and low-temperature frozen tissue preparation. It operates on classical rotary microtomy principles—where the specimen advances linearly toward a fixed, ultra-sharp knife during each handwheel rotation—combined with a thermoelectric (Peltier) cryogenic system that enables rapid, stable temperature control of both specimen stage and knife. This architecture supports reproducible sectioning across heterogeneous biological matrices—including soft neural tissues, fibrous plant specimens, calcified bone segments, and clinical biopsy samples—without requiring separate dedicated instruments for paraffin and frozen protocols. Designed to meet routine academic, diagnostic pathology, and preclinical research demands, the JY-202A complies with fundamental mechanical safety and ergonomic standards applicable to Class I laboratory microtomes under ISO 13485-aligned manufacturing practices.

Key Features

• Simultaneous paraffin and cryosectioning capability via integrated Peltier-based cooling module with independent thermal regulation of specimen stage and knife
• Precision micrometer feed mechanism offering 1 µm incremental adjustment across 1–25 µm thickness range, with mechanical repeatability validated at ±5% tolerance
• Sealed, box-type protective housing minimizing particulate ingress and safeguarding operator interaction zones from accidental contact or contamination
• Tool-free, quick-release specimen holder system enabling rapid interchange between standard brass chucks, plant tissue adapters, and bone-holding fixtures
• Dual-channel LCD display showing real-time and setpoint temperatures for both stage and cold knife, with automatic defrost cycle initiation and seamless re-cooling recovery
• Mechanical travel limit switch with audible/visual alarm to prevent over-travel damage; manual handwheel lock at any rotational position for precise specimen alignment
• Auto-retraction function after each section, reducing knife wear and improving ribbon consistency; programmable slice counter with non-volatile memory retention

Sample Compatibility & Compliance

The JY-202A accommodates specimens up to 40 × 30 mm in cross-section, supporting standard cassette-mounted paraffin blocks as well as unembedded or OCT-embedded frozen tissues. Its 45° knife angle optimizes cutting efficiency for low-adhesion materials while minimizing compression artifacts in delicate epithelial or vascular structures. The system is routinely deployed in GLP-compliant histology labs where traceability and procedural consistency are required; although not certified to FDA 21 CFR Part 11 out-of-the-box, its manual operation mode, physical calibration locks, and absence of networked data transmission render it inherently suitable for environments governed by ISO/IEC 17025 or CAP-accredited workflows. All mechanical adjustments are fully documented in the user manual per ASTM E2915-22 guidelines for microtome verification.

Software & Data Management

The JY-202A operates without embedded firmware or proprietary software—its controls are entirely electromechanical and analog-display based. Temperature settings, slice counts, and defrost cycles are stored in onboard non-volatile memory and persist through power interruption. No USB, Bluetooth, or Wi-Fi interfaces are present, eliminating cybersecurity concerns and ensuring long-term compatibility with legacy lab information systems (LIS). For audit readiness, all operational parameters—including date-stamped slice count logs and thermal deviation records—can be manually transcribed into electronic lab notebooks (ELN) compliant with ALCOA+ principles. Optional accessories include calibrated reference blocks for periodic performance verification per CLSI EP15-A3.

Applications

This microtome serves core applications across multiple domains: routine H&E staining of human surgical biopsies in clinical pathology labs; serial sectioning of rodent brain tissue for immunohistochemistry in neuroscience studies; high-fidelity cross-sectional slicing of lignified plant stems for anatomical analysis; and semi-thin sectioning of decalcified bone specimens prior to von Kossa or TRAP staining. Its rapid cooldown (<8 min to −25 °C) and stable thermal hold make it especially effective for lipid-rich tissues prone to ice crystal artifact formation. The modular design also supports method development for novel embedding media such as Lowicryl or LR White resins when used with appropriate accessory chucks.

FAQ

Is the JY-202A compatible with standard disposable microtome blades?
Yes—it accepts universal single-edge steel blades (e.g., Feather S35, Leica 818) via optional blade holders; the base configuration includes a reusable tungsten-carbide knife mount.
Does the instrument support GMP documentation requirements?
While no electronic audit trail is generated, all mechanical calibrations and maintenance intervals can be recorded manually in accordance with Annex 11 and ISO 9001:2015 clause 7.5.3.
What is the maximum achievable freezing temperature at the specimen stage?
The cryo module achieves ≤ −30 °C at the stage surface under ambient conditions of 25 °C and 50% RH, with ≥60 °C differential relative to environment.
Can the JY-202A perform semi-thin sections for electron microscopy?
It is rated for 1–25 µm sections; for ultrathin EM sections (50–100 nm), a dedicated ultramicrotome is required.
Is service and calibration support available outside China?
DS provides technical documentation in English and partners with regional distributors for field service; calibration tools and torque specifications are included in the shipment package.