Eppendorf Multipette E3/E3x Electronic Repeater Pipette

Overview

The Eppendorf Multipette E3/E3x is an advanced electronic repeater pipette engineered for high-precision, high-reproducibility liquid handling in demanding laboratory environments. Unlike air-cushion pipettes, it operates on the external piston (positive displacement) principle—where the piston makes direct contact with the liquid via a disposable Combitip advanced tip. This eliminates compressible air gaps and ensures consistent volumetric delivery across challenging fluid classes: viscous liquids (e.g., glycerol, PEG solutions), volatile solvents (e.g., acetone, chloroform), foaming reagents (e.g., detergents, serum-containing media), and hazardous substances (including toxic, radioactive, or biohazardous samples). Designed and manufactured in Hamburg, Germany, the Multipette E3/E3x meets stringent quality control standards aligned with ISO 8655-2 for positive displacement pipettes and supports traceable calibration under GLP/GMP frameworks.

Key Features

• Motor-driven operation with brushless DC motor for smooth, low-vibration dispensing and reduced user fatigue during extended protocols.
• Seven programmable liquid handling modes: Pipetting, Dispensing, Automatic Dispensing, Sequential Dispensing, Aspirate & Dispense, Titration, and Multiple Aspiration—each configurable via intuitive rotary selector dial.
• Ergonomic PhysioCare design validated per ISO 9241-5: optimized button size, tactile feedback, weight distribution (≤270 g), and balanced center of gravity to minimize repetitive strain injury (RSI) risk during multi-hour workflows.
• Integrated volume memory with up to 99 programmable volume presets; real-time display shows current volume, mode, and battery status.
• Rechargeable lithium-ion battery supporting >1,000 full dispense cycles per charge (50 mL at 1 mL increments); USB-C charging port enables rapid top-up without interrupting workflow.
• IP54-rated housing resists dust ingress and incidental splashes, suitable for cleanroom Class 10,000 (ISO 7) environments.

Sample Compatibility & Compliance

The Multipette E3/E3x achieves reliable performance across diverse sample matrices without requiring tip recalibration or manual compensation. Its positive displacement architecture inherently mitigates evaporation losses in volatile compounds and prevents aerosol generation from infectious agents—critical for BSL-2/BSL-3 applications. All Combitips advanced tips are certified DNase/RNase-free, non-pyrogenic, and sterilized by gamma irradiation (SAL 10⁻⁶). Device firmware complies with IEC 61326-1 (EMC) and IEC 61010-1 (safety), and supports audit trails required under FDA 21 CFR Part 11 when integrated with Eppendorf’s VisioNize Lab Suite software. Calibration certificates conform to ISO/IEC 17025-accredited laboratories and reference standards traceable to PTB (Physikalisch-Technische Bundesanstalt).

Software & Data Management

When paired with Eppendorf VisioNize Lab Suite (v3.0+), the Multipette E3/E3x enables full digital workflow integration. Users can remotely configure dispensing protocols, export timestamped operation logs (including volume, mode, date/time, operator ID), and generate PDF calibration reports compliant with ISO 17025 documentation requirements. The system supports role-based access control and electronic signatures for regulated environments. All data is encrypted at rest and in transit (AES-256), satisfying GDPR and HIPAA-aligned data governance policies. Firmware updates are delivered over-the-air via secure HTTPS channels, ensuring continuous alignment with evolving regulatory expectations.

Applications

• High-throughput assay setup in drug discovery (e.g., ELISA plate filling, qPCR master mix preparation).
• Precision dilution series generation for analytical standards in environmental testing and clinical diagnostics.
• Safe handling of cytotoxic chemotherapeutics in oncology research labs.
• Consistent dispensing of viscous cell culture matrices (e.g., Matrigel®, collagen I solutions) without clogging or volume drift.
• Regulatory-compliant sample aliquoting for biobanking and biorepository operations under ISO 20387 accreditation.
• Quality control testing of raw materials in pharmaceutical manufacturing (ICH Q5, Q7).

FAQ

What types of tips are compatible with the Multipette E3/E3x?
Only Eppendorf Combitips advanced (1–50 mL) and Combitips plus (0.1–10 mL) are supported. Third-party tips are not validated and may compromise accuracy or void warranty.
Can the device be calibrated in-house?
Yes—users may perform routine performance verification using gravimetric methods per ISO 8655-6. Full calibration must be conducted by an Eppendorf-certified service technician or ISO/IEC 17025-accredited lab.
Is the Multipette E3/E3x suitable for use in laminar flow hoods?
Yes—the IP54 rating and absence of internal air pathways prevent contamination ingress; however, avoid direct UV exposure to the display unit to preserve OLED longevity.
How does the E3x differ from the E3 model?
The E3x adds Bluetooth 5.0 connectivity, expanded memory (99 vs. 49 presets), and enhanced firmware support for VisioNize Lab Suite v3.0+ features including remote diagnostics and predictive maintenance alerts.
Does the device support GLP-compliant electronic records?
Yes—when used with VisioNize Lab Suite and configured with audit trail, electronic signature, and data integrity settings enabled, it fulfills ALCOA+ principles for regulated documentation.