FLUKO FA25 High-Shear Dispersing Homogenizer

Overview

The FLUKO FA25 High-Shear Dispersing Homogenizer is an engineered laboratory-scale rotor-stator system designed for reproducible, scalable high-intensity mixing, emulsification, dispersion, and cell disruption. Operating on the principle of controlled hydrodynamic shear generated between a rapidly rotating probe tip and a stationary stator sleeve, the FA25 delivers consistent energy input across variable sample volumes—from microliter-scale tissue homogenization to liter-scale formulation development. Its modular architecture supports rapid interchangeability of standardized dispersing heads (e.g., 6G, 8G, 10G, 18F, 25F), enabling method transfer from early-stage R&D to pilot-scale process optimization. The device complies with general laboratory safety standards (IEC 61010-1) and is constructed to support GLP-aligned documentation practices through traceable speed calibration and stable mechanical performance under continuous duty cycles.

Key Features

• 500 W brushless motor delivering stable torque across the full speed range (10,000–28,000 rpm), with digital RPM feedback ensuring ±1% speed accuracy at setpoint
• Interchangeable SS316L dispersing heads—each optimized for specific volume ranges and rheological profiles—including fine-tip 6G (0.2–10 mL), tapered 10G (30–150 mL), and high-capacity 25F (100–5000 mL) configurations
• PTFE-coated shaft sleeves providing chemical resistance and low-friction operation during extended runs with viscous or abrasive media
• Ergonomic vertical stand with adjustable clamping mechanism (included) and integrated height lock for repeatable probe immersion depth control
• Thermal management system enabling uninterrupted operation up to 30 minutes at maximum speed without thermal shutdown under ambient conditions ≤35 °C
• CE-marked electrical design with overcurrent and overload protection circuits compliant with EN 61000-6-3 (EMC emission limits)

Sample Compatibility & Compliance

The FA25 accommodates aqueous, organic, and semi-solid matrices—including polymer solutions, nanoparticle suspensions, biological lysates, cosmetic emulsions, and pharmaceutical suspensions—within a viscosity range up to 8,000 cP. All wetted parts conform to USP Class VI biocompatibility requirements when using SS316L and PTFE components. While not certified for ISO 13485 or FDA 21 CFR Part 11 out-of-the-box, the instrument supports audit-ready workflows when paired with validated SOPs and external electronic lab notebook (ELN) integration. It meets standard laboratory environmental specifications per ISO 14644-1 Class 8 (100,000) cleanroom compatibility for non-sterile applications.

Software & Data Management

The FA25-D digital series integrates a microprocessor-controlled interface with real-time RPM display and memory recall for up to three user-defined protocols. Though it lacks native USB or Ethernet connectivity, its analog-compatible output signal (0–10 V DC proportional to actual speed) permits integration into centralized lab automation systems via PLC or DAQ modules. For regulatory environments, users may implement manual logbook entries or link to third-party data acquisition platforms supporting 21 CFR Part 11-compliant electronic signatures and audit trails. Firmware updates are performed locally via proprietary service port; no cloud dependency or remote access capability is included.

Applications

• Preparation of oil-in-water and water-in-oil emulsions for food, cosmetic, and pharmaceutical development
• Dispersion of nanomaterials (e.g., graphene oxide, TiO₂, SiO₂) in polymeric or surfactant-stabilized media
• Homogenization of animal and plant tissues for RNA/DNA extraction and proteomic sample preparation
• Deagglomeration of pigment pastes and ceramic slurries prior to rheological or particle size analysis
• Accelerated stability testing of suspension formulations under controlled shear stress profiles
• Scale-down modeling of industrial inline homogenizers for process validation studies

FAQ

What is the maximum recommended operating time at 28,000 rpm?
Continuous operation at maximum speed is rated for 30 minutes per cycle under ambient temperatures ≤35 °C and relative humidity ≤70%. Allow ≥15 minutes cooldown between consecutive high-speed runs.
Can the FA25 be used for sterile applications?
The unit itself is not autoclavable. However, all SS316L dispersing heads and PTFE sleeves can be sterilized separately via dry heat (160 °C, 2 h) or ethylene oxide gas. Sterility must be confirmed independently per ISO 11135 or ISO 11137 protocols.
Is calibration traceable to national standards?
RPM calibration is performed using a NIST-traceable optical tachometer during factory acceptance testing. A certificate of conformance (CoC) is supplied with each unit; full calibration reports require optional third-party metrology services.
How does the FA25 compare to ultrasonic homogenizers for cell lysis?
Rotor-stator systems like the FA25 generate bulk shear forces ideal for soft-tissue homogenization and coarse particle reduction. Ultrasonics provide higher localized cavitation energy but are less efficient for high-viscosity or air-sensitive samples. Method selection should be based on target particle size distribution, thermal sensitivity, and scalability requirements.
Are replacement parts available globally?
FLUKO maintains authorized distribution channels in North America, EMEA, and APAC. Critical spares—including dispersing heads, shaft sleeves, and drive couplings—are stocked with lead times ≤4 weeks. OEM part numbers (e.g., 032, 052, 22301) ensure cross-regional interchangeability.