Hanil Ultra 5.0 Ultracentrifuge

Overview

The Hanil Ultra 5.0 Ultracentrifuge is a high-performance, refrigerated benchtop ultracentrifuge engineered for demanding separation tasks in molecular biology, virology, nanomaterials research, and biopharmaceutical development. Utilizing precision-engineered brushless induction motor technology and active magnetic bearing support, the system delivers exceptional rotational stability at speeds up to 50,000 rpm—enabling sedimentation-based isolation of subcellular organelles, exosomes, lipoprotein fractions, viral particles, and synthetic nanoparticles. Its robust vacuum-sealed chamber minimizes aerodynamic drag and heat generation, while integrated refrigeration maintains sample integrity across the full operational temperature range (−20 °C to +40 °C). Designed and manufactured in South Korea under strict ISO 9001-certified production protocols, the Ultra 5.0 integrates European-derived mechanical architecture with localized quality control rigor honed over five decades of centrifuge engineering.

Key Features

• Carbon-fiber composite rotor construction—corrosion-resistant, non-metallic, and compatible with aggressive solvents and sterilization cycles including autoclaving at 121 °C for 20 minutes.
• High-dynamic drive system delivering rapid acceleration (0–50,000 rpm in ≤3.5 min) and controlled deceleration—reducing total run time without compromising pelleting efficiency or sample integrity.
• Lightweight, wear-free rotor mounting mechanism—eliminates mechanical wear associated with traditional metallic spindle interfaces and simplifies rotor exchange during multi-step workflows.
• Extended rotor warranty: 5-year limited warranty on all factory-certified rotors, reflecting structural validation under accelerated life-cycle testing per ASTM F2100-22 standards.
• Modular rotor compatibility—supports ten distinct rotor types (including fixed-angle, swinging-bucket, and vertical rotors), each validated for maximum speed, RCF, and load distribution per IEC 61010-2-020 safety directives.
• Fine-resolution control—1 rpm minimum speed increment and ±0.1 °C temperature resolution ensure reproducible protocol execution across GLP-compliant laboratories.

Sample Compatibility & Compliance

The Ultra 5.0 accommodates a broad spectrum of sample formats via flexible adapter systems—including 38.5 mL, 18.5 mL, and 1.5 mL tubes—enabling seamless transition between preparative ultracentrifugation and analytical-scale runs. All rotors are certified for use with standard polyallomer, polycarbonate, and stainless-steel tube materials compliant with USP Class VI and ISO 10993-5 cytotoxicity requirements. The instrument meets IEC 61010-1 (Ed. 3.1) for laboratory equipment safety, EN 61326-1 for electromagnetic compatibility, and carries CE marking for EU conformity. Temperature control functionality supports 21 CFR Part 11–compliant data logging when paired with validated third-party software platforms used in regulated biomanufacturing environments.

Software & Data Management

The front-panel touchscreen interface provides real-time visualization of rpm, RCF, elapsed time, chamber temperature, rotor identification, and safety interlock status. Twenty programmable methods can be stored locally with user-defined acceleration/deceleration profiles (10 each), enabling consistent method transfer across shifts or operators. While the Ultra 5.0 does not include built-in network connectivity, its RS-232 serial port supports integration with LIMS or ELN systems through industry-standard ASCII command protocols. Audit-trail-capable external software packages—such as Hanil CentriLog Pro (v3.2+)—enable electronic signature capture, version-controlled protocol archiving, and automated report generation aligned with FDA ALCOA+ principles.

Applications

• Isolation of extracellular vesicles (exosomes, microvesicles) from cell culture supernatants and biofluids using density-gradient or differential ultracentrifugation (e.g., sucrose or iodixanol gradients).
• Purification of adeno-associated virus (AAV) vectors and lentiviral particles for gene therapy process development.
• Subcellular fractionation of mitochondria, lysosomes, and endoplasmic reticulum membranes in neurodegenerative disease models.
• Characterization of colloidal nanoparticle suspensions—including gold, silica, and lipid nanoparticles—via pelleting efficiency analysis and size distribution correlation.
• Lipoprotein subclass separation (HDL, LDL, VLDL) in clinical lipidomics studies requiring high-precision RCF control and thermal stability.

FAQ

What is the maximum allowable imbalance tolerance for the Ultra 5.0?
The system features automatic imbalance detection with a threshold of ≤0.5 g difference between opposing tubes; operation halts immediately if exceeded.
Can the Ultra 5.0 operate without refrigeration?
Yes—the compressor can be disabled via firmware setting, allowing ambient-temperature operation where thermal stability is not required.
Are rotor certifications included with purchase?
Each rotor ships with a traceable calibration certificate, including dynamic balance verification, material certification, and speed/RCF validation test reports.
How is rotor lifetime monitored?
Rotors are assigned unique IDs logged into the instrument’s memory; cumulative runtime and maximum speed history are retained for compliance-driven lifecycle tracking.
Does Hanil provide installation qualification (IQ) documentation?
Yes—factory-issued IQ/OQ templates compliant with ISO/IEC 17025 and GMP Annex 15 are available upon request for qualified users.