Hanna HI98193 Portable Dissolved Oxygen (DO) and Biochemical Oxygen Demand (BOD) Meter

Overview

The Hanna HI98193 is a high-precision portable electrochemical meter engineered for field and laboratory measurement of dissolved oxygen (DO) and derived biochemical parameters—including Biochemical Oxygen Demand (BOD), Oxygen Uptake Rate (OUR), and Specific Oxygen Uptake Rate (SOUR). It employs Clark-type polarographic sensing technology with a membrane-covered cathode and silver/silver chloride reference electrode, enabling stable, low-drift DO quantification in aqueous matrices across diverse environmental and wastewater treatment applications. The instrument integrates automatic temperature compensation (0.0–50.0°C), barometric pressure compensation (450–850 mmHg), and salinity compensation (0–70 g/L), all critical for trace-level DO accuracy under variable ambient conditions. Its dual-display capability simultaneously reports DO concentration (mg/L or ppm) and percent saturation (%), facilitating rapid interpretation of oxygen availability relative to thermodynamic equilibrium. Designed in accordance with ISO 5814:2012 (water quality — determination of dissolved oxygen — electrochemical probe method) and aligned with ASTM D888 and USP principles for oxygen measurement, the HI98193 supports regulatory-compliant data acquisition in GLP- and GMP-aligned environments.

Key Features

• Integrated polarographic DO sensor (HI764073) with built-in thermistor for real-time temperature compensation and DIN connector for secure, low-noise signal transmission
• Automatic atmospheric pressure compensation via internal barometer or manual input—enabling altitude-corrected DO reporting per ISO 5814 requirements
• Direct BOD₅ calculation based on initial and final DO measurements, compliant with standard 5-day incubation at 20°C (ISO 5815-1:2019)
• Simultaneous calculation and display of OUR (mg O₂/L·h) and SOUR (mg O₂/g VSS·h), supporting activated sludge process monitoring and biokinetic assessment
• GLP-compliant data logging: stores date, time, calibration values (DO, pressure, temperature, salinity), and operator ID (via optional HI92000-15 software)
• IP67-rated housing and IP68-rated probe ensure operational integrity during extended field deployment in rain, splashing, or temporary submersion
• Backlit graphical LCD with multi-parameter layout—displaying DO, saturation %, atmospheric pressure, and temperature concurrently
• User-configurable auto-off intervals (5, 10, 30, 60 min or disabled) and ergonomic single-hand operation for prolonged handheld use

Sample Compatibility & Compliance

The HI98193 is validated for use with freshwater, seawater, wastewater effluents, activated sludge suspensions, and biological reactor samples. Its polarographic sensor design minimizes flow dependence and responds reliably in low-turbidity and moderately suspended solids matrices (<1000 mg/L TSS). For BOD₅ analysis, the instrument meets the functional requirements of EPA Method 410.4 and ISO 5815-1:2019 when paired with standardized incubation protocols and appropriate sample dilution. Pressure and salinity compensation algorithms are traceable to NIST-referenced standards and conform to IEC 61000-4 electromagnetic compatibility directives. The device’s firmware implements audit-trail-capable calibration logging, satisfying FDA 21 CFR Part 11 data integrity prerequisites when used with HI92000-15 software and electronic signature modules.

Software & Data Management

Data export and long-term traceability are enabled via USB interface and the HI92000-15 Windows-compatible software suite. This platform provides structured CSV/Excel export, time-series graphing, calibration history review, and GLP report generation—including full metadata (calibration date/time, pressure, temperature, salinity, electrode status). Each stored reading retains its contextual environmental parameters, ensuring reproducibility during third-party validation or regulatory audits. The software supports batch export of BOD, OUR, and SOUR datasets for integration into LIMS or statistical process control (SPC) platforms. All calibration events generate immutable timestamps and parameter snapshots, fulfilling ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) data governance expectations.

Applications

• Wastewater treatment plant process control: monitoring DO in aeration basins, calculating SOUR to assess microbial viability and sludge health
• Environmental field surveys: profiling DO stratification in lakes, rivers, and estuaries with altitude- and salinity-adjusted readings
• Drinking water distribution system verification: detecting anaerobic zones via localized DO depletion
• Research laboratories: kinetic studies of aerobic biodegradation, respirometry assays, and bioreactor optimization
• Educational settings: hands-on instruction in water quality standards, BOD methodology, and oxygen mass transfer fundamentals
• Aquaculture management: maintaining optimal DO thresholds for fish and shellfish health across varying salinities and temperatures

FAQ

How does the HI98193 compensate for altitude during DO measurement?
It uses either its integrated barometer (450–850 mmHg range) or manually entered pressure to adjust the theoretical saturation value—ensuring accurate mg/L conversion regardless of elevation.
Can the HI98193 perform BOD₅ testing without external incubation equipment?
No—it calculates BOD from user-entered initial and final DO values but requires a certified 20°C incubator and standardized 5-day holding period per ISO 5815-1.
What maintenance is required for the HI764073 polarographic probe?
Regular replacement of the HI76407A/P membrane cap and HI7041S electrolyte solution every 2–4 weeks (or after 50–100 measurements), plus periodic zero-point calibration using HI7040 zero-oxygen solution.
Is the HI98193 compliant with FDA 21 CFR Part 11 for electronic records?
Yes—when used with HI92000-15 software, electronic signatures, and audit-trail-enabled calibration logs, it satisfies Part 11 requirements for closed-system validation.
Does the instrument support custom units for pressure output?
Yes—it displays pressure in mmHg, inHg, atm, psi, kPa, or mbar, selectable via setup menu.