Hanuo FD-1E-50 Benchtop T-Shaped Manifold Freeze Dryer

Overview

The Hanuo FD-1E-50 is a compact, benchtop T-shaped manifold freeze dryer engineered for reliable, small-batch lyophilization in academic, pharmaceutical, and microbiological laboratories. It operates on the fundamental principle of sublimation—removing water from frozen samples under deep vacuum while maintaining sample integrity below the eutectic temperature. Designed specifically for ampoule-based preservation, the FD-1E-50 enables primary drying of heat-sensitive biological materials—including bacterial and fungal cultures, enzymes, antibodies, and diagnostic reagents—without thermal degradation. Its −50 °C condenser ensures efficient vapor capture during extended drying cycles, while the < 20 Pa ultimate vacuum supports consistent ice sublimation kinetics across heterogeneous sample loads. The system’s modular architecture integrates seamlessly into GLP-compliant workflows where traceability, repeatability, and minimal operator intervention are critical.

Key Features

• T-shaped stainless steel manifold with 24 independently controllable silicone stopcock valves—enabling parallel processing of multiple ampoules or vials without cross-contamination
• High-efficiency dual-stage refrigeration system achieving stable condenser temperatures of −50 °C, optimized for rapid ice trapping and reduced cycle time
• Built-in vacuum monitoring with analog gauge calibrated to ISO 27874 standards; compatible with optional digital vacuum controllers for logging
• Robust aluminum alloy main chamber housing with insulated door seal and reinforced viewport for real-time visual monitoring of drying progress
• Compact footprint (380 × 600 × 345 mm) designed for standard laboratory bench space, requiring no external cooling water or compressed air
• Integrated power management supporting continuous operation at 850 W nominal load under 220 V / 50 Hz supply—compliant with IEC 61000-3-2 harmonic emission limits

Sample Compatibility & Compliance

The FD-1E-50 is validated for use with standard 2–10 mL glass ampoules and serum vials (13–22 mm diameter), making it suitable for long-term microbial strain banking per ATCC and DSMZ guidelines. Its −50 °C condenser temperature meets the minimum requirement for lyophilizing most mesophilic and psychrotrophic microorganisms, including Bacillus subtilis, Saccharomyces cerevisiae, and Aspergillus niger. While not certified to ISO 22042 or FDA 21 CFR Part 11 out-of-the-box, the system supports audit-ready documentation when paired with external data loggers compliant with Annex 11 principles. All wetted parts contact-grade stainless steel (AISI 304) and silicone elastomers conform to USP Class VI biocompatibility testing requirements.

Software & Data Management

The FD-1E-50 operates as a manually controlled instrument without embedded firmware or touchscreen interface—intentionally designed to maximize operational transparency and reduce validation burden in regulated environments. Vacuum level and elapsed time are monitored via front-panel analog gauges and mechanical timers. For laboratories requiring electronic recordkeeping, the system interfaces with third-party USB or RS-485 vacuum transducers and temperature probes (e.g., PT100 sensors placed in shelf or condenser wells). Raw analog outputs can be logged using compliant SCADA platforms such as LabVIEW or DeltaV, supporting 21 CFR Part 11 audit trails when configured with electronic signatures and user access controls.

Applications

• Long-term preservation of microbial cultures in ampoules for culture collections, QC reference labs, and biosafety level-1/2 facilities
• Stabilization of labile proteins, monoclonal antibodies, and mRNA intermediates prior to analytical characterization or formulation studies
• Preparation of dried calibration standards for elemental analysis (ICP-MS, AAS) and chromatographic methods (HPLC, GC)
• Routine dehydration of botanical extracts, probiotic powders, and vaccine candidates during early-stage process development
• Teaching applications in food science, pharmacology, and microbiology curricula—demonstrating phase behavior, mass transfer limitations, and collapse temperature phenomena

FAQ

What types of containers are compatible with the FD-1E-50 manifold?
Standard 2–10 mL glass ampoules and 13–22 mm diameter serum vials with crimp-seal compatibility. Custom adapters available upon request.
Does the system include a vacuum pump?
Yes—the standard configuration includes a 2 L domestic oil-lubricated rotary vane vacuum pump rated for ≤20 Pa base pressure.
Can the FD-1E-50 be used for drying heat-sensitive peptides?
Yes—provided the product’s eutectic temperature is ≥−45 °C and shelf temperature remains below −30 °C during primary drying.
Is the condenser capacity sufficient for overnight runs?
With a 3 kg/24 h ice-trapping capacity, the −50 °C condenser supports uninterrupted operation for up to 16 hours with typical microbial pellet loads (≤500 mg dry weight per vial).
How is maintenance performed on the refrigeration system?
The sealed compressor unit requires no routine oil changes; annual inspection of refrigerant charge and condenser coil cleanliness is recommended per manufacturer service bulletin HB-FD1E-REV3.