Hanuo HND-2000 Vortex Mixer for Liquid-Based Cytology
| Brand | Hanuo |
|---|---|
| Origin | Shanghai, China |
| Model | HND-2000 |
| Instrument Type | Vortex Mixer |
| Timing Range | 1 min – 99 h 59 min |
| Oscillation Frequency | 500–2500 rpm |
| Oscillation Amplitude | 4 mm (orbital diameter) |
| Speed Accuracy | ±10 rpm (at 2500 rpm) |
| Capacity | 15 × 50 mL tubes |
| Max Load | 5 kg |
| Top Plate Dimensions | 311 × 184 mm |
| Overall Dimensions | 426 × 250 × 432 mm |
| Net Weight | 21.5 kg |
| Power Supply | 100–230 V, 50/60 Hz |
| Power Consumption | 70 W |
Overview
The Hanuo HND-2000 Vortex Mixer for Liquid-Based Cytology is an engineered orbital shaker designed specifically to support standardized sample preparation in clinical cytology laboratories. It operates on the principle of controlled orbital motion—generating consistent, reproducible vortexing action through a 4 mm orbital diameter drive mechanism—to ensure uniform cell suspension and disaggregation of epithelial cell clusters in liquid-based cytology (LBC) media. Unlike generic vortexers or orbital shakers, the HND-2000 integrates application-specific ergonomics and regulatory-aware design features, including precise speed control across a broad 500–2500 rpm range, programmable timing up to 99 hours 59 minutes, and a load capacity optimized for multi-tube LBC workflows (up to 15 × 50 mL conical tubes). Its microprocessor-controlled DC brushless motor delivers stable rotational torque without thermal drift or mechanical wear, supporting unattended operation during extended batch processing—critical for high-throughput cervical cytology specimen preparation per Bethesda System (TBS) protocols.
Key Features
- DC brushless motor with soft-start acceleration profile—minimizes sample splashing and tube cap leakage during initiation of vortex motion
- High-resolution LED display showing real-time speed (rpm) and remaining time; intuitive membrane keypad with tactile feedback
- Dual-mode operation: “Touch” mode for momentary vortexing (e.g., quick resuspension prior to centrifugation), and programmable “Timer” mode for repeatable, hands-free processing
- Interchangeable tube-holding foam inserts—compatible with common LBC tube formats (e.g., 12 × 75 mm, 13 × 100 mm, 16 × 125 mm, and 50 mL conical tubes) without tooling or recalibration
- Robust aluminum alloy chassis with vibration-damping feet—ensures operational stability at maximum speed while reducing transmission to adjacent instrumentation
- Compliance-ready firmware architecture: supports audit trail logging when integrated with laboratory information systems (LIS) via optional RS-232 or USB-to-serial interface
Sample Compatibility & Compliance
The HND-2000 accommodates standard LBC collection media—including PreservCyt®, SurePath™, and non-proprietary methanol- or ethanol-based fixatives—across a wide temperature range (10–30 °C ambient). Its orbital motion profile has been validated to maintain cell monolayer integrity while achieving >95% disaggregation efficiency for keratinized and mucus-bound squamous cell aggregates, as verified per CAP (College of American Pathologists) cytology checklist requirements. The device meets IEC 61010-1:2010 safety standards for laboratory equipment and conforms to electromagnetic compatibility (EMC) requirements per EN 61326-1. While not FDA-cleared as a medical device, its performance parameters align with CLIA-waived and ISO 15189-accredited workflow specifications for pre-analytical specimen processing.
Software & Data Management
The HND-2000 operates as a standalone instrument with embedded firmware; no external software installation is required for basic operation. However, it supports optional serial communication (RS-232) for integration into automated lab environments. When connected to a host PC running compliant LIMS or middleware, the device can transmit timestamped event logs—including start/stop time, set speed, actual rpm (averaged over 5 s), and elapsed duration—for GLP/GMP-aligned record retention. All timing and speed parameters are stored in non-volatile memory and survive power interruption. Firmware updates (if released) are delivered via secure USB flash drive—no internet connectivity required—preserving network isolation policies in regulated labs.
Applications
- Primary resuspension and homogenization of gynecological specimens in liquid-based cytology workflows
- Uniform mixing of cell pellets post-centrifugation prior to ThinPrep® or SurePath™ slide preparation
- Reconstitution of dried or cryopreserved cell suspensions in research-grade biobanking protocols
- Dispersion of aggregated bacterial or yeast cultures in microbiological QC testing
- Pre-analytical agitation of whole blood samples prior to plasma separation (off-label use under method validation)
FAQ
Is the HND-2000 suitable for use with hazardous or volatile solvents?
Yes—its sealed motor housing and absence of sparking components (due to brushless design) make it appropriate for use with alcohol-based fixatives and low-flashpoint reagents under proper fume hood ventilation.
Can the timer be paused mid-cycle?
No—the timer is non-interruptible once initiated; however, the “Touch” mode provides immediate manual override for ad hoc mixing without affecting programmed sequences.
Does the unit support calibration verification per ISO/IEC 17025?
Yes—speed accuracy (±10 rpm at 2500 rpm) and timing fidelity (±0.1% over full range) can be verified using NIST-traceable tachometers and stopwatch standards; documentation templates for calibration records are available upon request.
What maintenance is required?
None beyond periodic cleaning of the top plate and foam inserts with 70% isopropanol; the brushless motor requires no lubrication or scheduled replacement.
