Hanuo HNX-1500 Microplate Orbital Shaker
| Brand | Hanuo |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | HNX-1500 |
| Price | USD 760 (est.) |
| Instrument Type | Microplate Shaker |
| Timer Range | 0–99 h 59 min |
| Oscillation Speed | 300–1500 rpm |
| Amplitude | 3 mm (orbital) |
| Temperature Control | None |
| Capacity | 4 standard microplates (e.g., 96-well, 384-well, 24-well, 48-well plates) |
| Dimensions | 284 × 264 × 121 mm |
| Weight | 6 kg |
Overview
The Hanuo HNX-1500 Microplate Orbital Shaker is an engineered solution for precise, reproducible mixing of low-volume samples in multiwell formats. Designed specifically for life science laboratories performing ELISA, cell-based assays, immunostaining, and molecular binding studies, this shaker employs orbital motion—horizontal circular oscillation at a fixed 3 mm amplitude—to generate uniform fluid shear across all wells without splashing or cross-contamination. Its brushless DC motor ensures consistent rotational torque across the full speed range (300–1500 rpm), eliminating commutator wear and enabling long-term operational stability under continuous use. Unlike heated or refrigerated shakers, the HNX-1500 operates at ambient temperature, making it suitable for placement inside incubators, cold rooms, or laminar flow hoods where thermal regulation is externally managed. Its compact footprint (284 × 264 × 121 mm) and 6 kg mass provide mechanical inertia sufficient to dampen resonance during high-speed operation while maintaining compatibility with standard laboratory benchtop configurations.
Key Features
- Brushless DC motor drive: Delivers maintenance-free operation, extended service life (>20,000 hours MTBF), and minimal electromagnetic interference—critical for sensitive detection systems nearby.
- Microprocessor-controlled timing and speed regulation: Achieves ±2 rpm speed accuracy and <±1% speed fluctuation over time, ensuring assay repeatability across multiple runs.
- Four-plate capacity: Accommodates up to four standard microplates simultaneously—including 96-well, 384-well, 24-well, and 48-well formats—without requiring adapters or reconfiguration.
- LED digital display interface: Shows real-time elapsed time, remaining time, and setpoint rpm with 1 rpm resolution; supports intuitive parameter entry via dual membrane buttons.
- Programmable timer with audible end-of-cycle alert: Supports durations from 1 minute to 99 hours 59 minutes; alarm tone activates automatically upon completion to prevent sample over-incubation.
- Low-noise design (<45 dB(A) at 1 m): Engineered with vibration-damping feet and balanced eccentric drive mechanism to meet ISO 14040-compliant acoustic requirements for shared lab environments.
Sample Compatibility & Compliance
The HNX-1500 is validated for use with ANSI/SLAS-standard microplates (including ANSI/SBS-1–2016 compliant 96- and 384-well plates) and common cell culture plates (e.g., Corning Costar, Thermo Fisher Nunc). Its orbital motion profile ensures homogeneous mixing of volumes ranging from 50 µL to 300 µL per well, minimizing edge effects and meniscus distortion. The unit complies with IEC 61010-1:2010 for electrical safety in laboratory equipment and meets EMC Directive 2014/30/EU for electromagnetic compatibility. While not certified for GLP/GMP production environments, its stable performance and traceable parameter logging (via optional RS-232 output) support adherence to internal SOPs aligned with ISO/IEC 17025 quality management principles.
Software & Data Management
The HNX-1500 operates as a standalone instrument with no embedded software stack. All settings are retained in non-volatile memory after power cycling. For integration into automated workflows, an optional RS-232 serial interface enables remote control and status monitoring using LabVIEW-compatible SCPI commands or custom Python scripts (e.g., PySerial). Time-stamped run logs—including start time, duration, final rpm, and cycle completion flag—can be exported for audit trail generation. Though not FDA 21 CFR Part 11 compliant out-of-the-box, the device supports electronic record retention when paired with validated third-party LIMS platforms that enforce user authentication and audit trail integrity.
Applications
- ELISA and immunoassay plate washing and incubation steps requiring gentle yet thorough reagent mixing.
- Cell attachment enhancement in coating protocols prior to seeding (e.g., fibronectin or poly-L-lysine application).
- Hybridization steps in nucleic acid detection assays (e.g., DNA/RNA probe binding on membrane or plate-based arrays).
- Antibody labeling reactions where controlled agitation prevents precipitation of conjugated proteins.
- High-throughput screening (HTS) pre-read mixing to ensure uniform ligand-receptor interaction kinetics across assay plates.
FAQ
Can the HNX-1500 be used inside a CO₂ incubator?
Yes—the unit is rated for operation at 5–40°C and relative humidity up to 80% non-condensing, making it compatible with most standard CO₂ incubators. Ensure adequate airflow around the base to prevent heat buildup.
Is speed calibration traceable to NIST standards?
Factory calibration is performed using a laser tachometer referenced to an internal quartz oscillator; NIST-traceable calibration certificates are available upon request at additional cost.
Does the shaker support staggered timing for multiple plates?
No—the timer controls all four plate positions uniformly. Independent control per plate requires external sequencing hardware or multi-channel programmable shakers.
What maintenance is required?
None beyond periodic cleaning of the platform surface with 70% ethanol; the brushless motor contains no consumable parts and requires no lubrication or alignment.
Can I connect multiple HNX-1500 units to one computer?
Yes—each unit has a unique RS-232 address setting (DIP switch configurable); up to 16 units can be daisy-chained via RS-485 converters with appropriate protocol handling.
