Hanuo SHA-CA Water Bath Constant-Temperature Orbital Shaker

Overview

The Hanuo SHA-CA is a precision-engineered water bath constant-temperature orbital shaker designed for laboratory applications requiring simultaneous thermal control and mechanical agitation under submerged conditions. It operates on the principle of reciprocating linear oscillation within a thermostatically regulated water bath, ensuring uniform heat transfer to samples via conduction and convection—critical for maintaining physiological or reaction-specific temperatures during dynamic incubation. Unlike air-jacketed shakers, the water bath configuration delivers superior temperature homogeneity (<±0.5 °C), reduced thermal lag, and minimized evaporation in open-vessel protocols such as bacterial suspension culture, enzymatic assays, and hybridization kinetics. Its digital microprocessor-based controller enables independent, real-time regulation of both temperature and oscillation speed—making it suitable for reproducible execution of time-sensitive protocols compliant with GLP and ISO/IEC 17025 frameworks.

Key Features

• Digital dual-control system with independent LED displays for temperature (°C) and rotational speed (rpm), enabling precise parameter setting and monitoring without cross-interference.
• Reciprocating oscillation mechanism with smooth, low-vibration motion profile—engineered to prevent splashing or foam formation in aqueous samples, preserving sample integrity and minimizing contamination risk.
• Stainless steel 304 water bath chamber and platform offering high corrosion resistance, easy cleaning, and long-term durability in humid and chemically exposed environments.
• Adjustable amplitude of 20 mm ensures compatibility with standard Erlenmeyer flasks (50–1000 mL), test tubes, and multi-well plates when mounted on spring-loaded clamps or universal platforms.
• Programmable timer (0–120 minutes) with automatic shutdown function—supports unattended operation while meeting safety requirements for extended incubation cycles.
• Compact footprint (71 × 55 × 49 cm) optimized for benchtop integration in biosafety cabinets, cold rooms, or shared core facilities without compromising accessibility or serviceability.

Sample Compatibility & Compliance

The SHA-CA accommodates a wide range of biological and biochemical sample formats—including liquid cultures in glass or PETG flasks, enzyme reaction mixtures in cuvettes, DNA hybridization vessels, and tissue dissociation suspensions. Its water bath design supports both sealed and vented containers, provided vapor pressure remains below operational temperature limits. The unit complies with IEC 61010-1:2010 safety standards for laboratory electrical equipment and meets electromagnetic compatibility (EMC) Class B requirements per EN 61326-1. While not certified for medical device use under FDA 21 CFR Part 820, its performance characteristics align with common validation criteria referenced in USP , ASTM E2500, and ISO 13485 Annex A for ancillary lab instrumentation used in R&D and QC environments.

Software & Data Management

The SHA-CA operates as a standalone analog-digital hybrid instrument with no embedded firmware or network interface. All operational parameters are set locally via tactile membrane buttons and verified through real-time LED readouts. For laboratories requiring audit-ready documentation, external data loggers (e.g., Omega OM-DAQPRO-5300 series) can be connected via optional PT100 probe outputs (available upon request) to record temperature and runtime traces. The absence of proprietary software reduces cybersecurity exposure and simplifies 21 CFR Part 11 compliance when paired with validated third-party LIMS or ELN systems that manage electronic signatures and change control workflows.

Applications

• Bacterial and yeast culture expansion under controlled aeration and thermal conditions (e.g., LB broth incubation at 37 °C, 150 rpm).
• Enzyme kinetics studies where substrate solubility, conformational stability, and reaction rate are temperature-dependent.
• Nucleic acid hybridization (Southern/Northern blotting) requiring consistent thermal agitation to accelerate probe binding kinetics.
• Protein solubilization and refolding protocols involving denaturant gradient dialysis in stirred water baths.
• Environmental microbiology assays, including MPN enumeration and biodegradation rate testing under standardized OECD 301 series conditions.
• Pharmaceutical excipient dispersion testing and dissolution profile development per USP .

FAQ

Does the SHA-CA support both reciprocating and orbital motion modes?
No—the SHA-CA is configured exclusively for reciprocating (linear back-and-forth) oscillation. For orbital motion, consider the THZ-82A or SHA-BA variants.
Is the water bath depth adjustable?
No—the bath depth is fixed at approximately 12 cm; users must select appropriate vessel height to ensure adequate submersion without overflow.
Can this unit be used with corrosive solutions like strong acids or organic solvents?
Not recommended—the stainless steel chamber resists mild aqueous corrosion but is not rated for prolonged exposure to halogenated solvents, concentrated HCl/HNO₃, or alkaline hydroxides above pH 12.
What is the maximum load capacity per platform?
The platform supports up to 15 kg evenly distributed—equivalent to four 2-L flasks filled to 75% capacity.
Is calibration documentation included with shipment?
Factory calibration certificates for temperature and speed sensors are available upon request and may be supplied with NIST-traceable references for an additional fee.