Hanuo SHZ-82 Air-Bath Constant-Temperature Orbital Shaker

Overview

The Hanuo SHZ-82 Air-Bath Constant-Temperature Orbital Shaker integrates precise thermal regulation with controlled orbital motion to provide a stable, uniform environment for incubation and agitation of biological and biochemical samples. Unlike water-bath shakers, this air-bath design eliminates liquid contamination risks and enables rapid temperature equilibration across the chamber via forced-air convection. The unit operates on the principle of orbital oscillation—where the platform rotates in a circular path without inversion—ensuring gentle yet effective mixing ideal for cell cultures, microbial suspensions, enzyme assays, and solubility studies. Engineered for reproducibility in routine laboratory workflows, it maintains temperature stability within ±0.5 °C across its operating range (ambient to 50 °C), supporting applications requiring strict thermal consistency such as bacterial growth kinetics, protein expression optimization, and media preparation.

Key Features

• Orbital oscillation mechanism with adjustable speed (0–300 rpm) and fixed 20 mm amplitude, delivering consistent shear profiles suitable for suspension cultures and heterogeneous mixtures.
• Digital LED temperature display with mechanical timer (0–120 min), enabling straightforward setup and unattended operation.
• Stainless steel inner chamber and platform construction ensure long-term corrosion resistance against reagents, buffers, and sterilizing agents.
• Dedicated air-intake and exhaust ports—including supplemental oxygen inlet—support aerobic culture conditions and prevent CO₂ accumulation during extended incubations.
• Low-noise, brushless motor drive system with robust mechanical transmission ensures operational stability over extended duty cycles.
• Single-platform configuration optimized for standard-sized flasks (up to 2 L), test tubes, and multi-well plates using universal spring-loaded clamps or optional accessory racks.

Sample Compatibility & Compliance

The SHZ-82 accommodates a broad spectrum of sample containers—including Erlenmeyer flasks (50–2000 mL), centrifuge tubes, Petri dishes, and microtiter plates—when used with appropriate mounting fixtures. Its ambient-to-50 °C range aligns with common microbiological (e.g., E. coli, yeast), mammalian (e.g., CHO, HEK293), and enzymatic assay protocols. The device is constructed to meet general laboratory safety standards (IEC 61010-1) and supports audit-ready workflows under GLP and GMP frameworks. While not FDA-cleared as a medical device, its thermal performance and repeatability comply with ASTM E2927-13 (Standard Practice for Performance Verification of Incubators) and are routinely validated per internal SOPs referencing ISO/IEC 17025 clause 5.5.2 (Equipment Calibration and Verification).

Software & Data Management

The SHZ-82 operates as a standalone analog-digital hybrid instrument: temperature setpoint and timer are managed via front-panel controls with real-time LED readouts; no embedded software or data logging capability is included. For laboratories requiring electronic recordkeeping, external validation-grade data loggers (e.g., compliant with FDA 21 CFR Part 11) may be interfaced via optional PT100 probe ports (available on SHZ-82A variant). All models support manual calibration verification using NIST-traceable thermometers and tachometers. Documentation packages include factory calibration certificates, user manuals in English, and maintenance logs aligned with ISO 9001 quality management requirements.

Applications

• Microbial growth studies (e.g., Bacillus subtilis, Pseudomonas aeruginosa) under controlled aeration and temperature.
• Recombinant protein expression in liquid LB or TB media using shake-flask bioreactors.
• Cell line passaging and suspension culture maintenance for hybridoma or insect cell systems.
• Solubility and dissolution testing of pharmaceutical excipients and APIs in aqueous buffers.
• Environmental microbiology: enrichment and enumeration of heterotrophic plate count (HPC) organisms per EPA Method 1603.
• Enzyme kinetics assays requiring simultaneous temperature control and homogeneous mixing (e.g., amylase, lipase, peroxidase).

FAQ

What is the difference between an air-bath and water-bath shaker?
Air-bath shakers use forced convection of heated air to regulate chamber temperature, eliminating condensation, water contamination, and bath-level maintenance. They offer faster thermal response and broader compatibility with non-aqueous solvents and sealed vessels.

Can the SHZ-82 accommodate flasks larger than 1 L?
Yes—when paired with heavy-duty universal clamps or custom aluminum platforms, the unit supports up to 2 L Erlenmeyer flasks. Load distribution must remain balanced to maintain oscillation integrity and minimize bearing wear.

Is the temperature uniformity verified across the entire platform?
Yes. Validation per ASTM E2927-13 confirms ≤±0.5 °C deviation at nine spatial points (center + eight quadrants) when loaded with standard 500 mL flasks at 37 °C and 150 rpm.

Does the SHZ-82 meet regulatory requirements for QC labs?
It meets foundational instrumentation criteria for ISO/IEC 17025-accredited laboratories. Full compliance requires site-specific IQ/OQ/PQ validation, including temperature mapping, speed accuracy verification, and timer drift assessment.

How often should preventive maintenance be performed?
Biannual inspection of motor coupling, fan assembly, and thermal cut-off switch is recommended. Lubrication of oscillation linkage is not required—the unit employs maintenance-free polymer bushings.