Hanuo SPE-24 Manual Solid Phase Extraction System

Overview

The Hanuo SPE-24 Manual Solid Phase Extraction System is an engineered laboratory manifold designed for reproducible, scalable sample preparation via solid-phase extraction (SPE). It operates on the principle of selective adsorption—where analytes in liquid samples are retained on solid sorbent cartridges based on polarity, ion exchange, or hydrophobic interactions, while matrix interferences pass through. Subsequent elution with optimized solvents enables targeted analyte recovery and concentration. This system supports standardized, low-risk SPE protocols across regulated and research environments, delivering consistent flow dynamics and minimal carryover without reliance on electronic actuation or programmable logic.

Key Features

• 24-position parallel processing manifold with individual stainless-steel vacuum control valves per channel—enabling precise, independent regulation of solvent flow rate during conditioning, loading, washing, and elution steps.
• One-piece molded borosilicate glass vacuum chamber with enhanced wall thickness and chemical resistance—certified for compatibility with acetonitrile, methanol, dichloromethane, ethyl acetate, and dilute acidic/basic aqueous solutions (pH 2–12).
• Ergonomic spiral-height-adjustable support rack accommodates collection tubes ranging from 13 mm to 30 mm diameter—including standard 15 mL and 50 mL conical centrifuge tubes, 4 mL vials, and custom-format SPE reservoirs.
• High-molecular-weight polymer cartridge tray—non-porous, non-absorbing, and dimensionally stable under continuous vacuum loads up to 0.08 MPa; resistant to deformation after >10,000 operational cycles.
• Integrated anti-fogging and cross-contamination mitigation design: sealed vacuum chamber lid with silicone gasket, recessed cartridge seating, and directional venting to prevent aerosol migration between adjacent channels.
• Modular accessory compatibility—including optional large-capacity solvent collection flasks (up to 2 L), nitrogen-assisted evaporation concentrators, and vacuum gauge kits with analog pressure readout (0–0.1 MPa range).

Sample Compatibility & Compliance

The SPE-24 accommodates all commercially available 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges—including silica-, C18-, SCX-, SAX-, Florisil-, and mixed-mode sorbents—from leading vendors (e.g., Waters, Agilent, Thermo Fisher, Phenomenex). Its mechanical architecture meets essential requirements for GLP-compliant sample prep workflows under ISO/IEC 17025-accredited laboratories. The system supports method validation per EPA Method 502.2 (halogenated organics in water), ASTM D7293 (pesticide residue analysis), and USP chromatographic separation guidance. All wetted materials comply with USP Class VI biocompatibility standards and FDA 21 CFR 177.2440 (plastics for food contact).

Software & Data Management

As a fully manual, non-electronic platform, the Hanuo SPE-24 does not incorporate embedded firmware, touchscreens, or digital data logging. This architecture eliminates software qualification burdens in GMP-regulated settings and ensures long-term operational continuity without dependency on firmware updates or cybersecurity maintenance. Users document parameters—including vacuum level, dwell times, solvent volumes, and cartridge lot numbers—via paper-based or LIMS-integrated electronic lab notebooks (ELN). Traceability is maintained through standardized SOP templates aligned with Annex 11 and ALCOA+ principles.

Applications

The SPE-24 serves as a foundational tool in environmental monitoring (e.g., trace pharmaceuticals in wastewater per EU Water Framework Directive), food safety testing (mycotoxin extraction from cereals per AOAC 2007.01), clinical toxicology (urinary amphetamines and opioids prior to LC-MS/MS), forensic drug screening (blood plasma cleanup for GC-MS confirmation), and pharmaceutical stability studies (degradant isolation from accelerated degradation matrices). Its robustness supports high-throughput batch processing in QC labs performing ≥50 extractions/day across multiple analytical platforms.

FAQ

Is the SPE-24 compatible with positive-pressure nitrogen manifolds?
Yes—when used with an external nitrogen regulator and pressure-controlled inlet adapter (sold separately), the vacuum chamber can be repurposed for positive-pressure elution at ≤60 psi.
What vacuum pump specifications are recommended?
A two-stage oil-lubricated rotary vane pump with ultimate vacuum ≤0.05 MPa and flow rate ≥25 L/min is optimal for full 24-channel operation under standard solvent viscosities.
Can I use this system for online SPE coupling with HPLC?
No—the SPE-24 is strictly an offline, batch-mode extraction manifold and lacks fluidic interfaces, pressure sensors, or valve actuators required for automated online integration.
Are replacement trays and glass chambers available as spare parts?
Yes—Hanuo supplies certified OEM replacement trays (P/N HT-SPE24-TRAY), vacuum chambers (P/N HT-SPE24-GLASS), and valve assemblies (P/N HT-SPE24-VALVEKIT) with documented material traceability.
Does the system meet CE or UL safety certification?
It is classified as a Class I laboratory apparatus under IEC 61010-1:2010 and carries a Declaration of Conformity for electromagnetic compatibility (EMC) and electrical safety—though it does not require CE marking as it contains no active electronic components.