HeHuo HH.CP-T Carbon Dioxide Incubator
| Brand | HeHuo (Jihepu) |
|---|---|
| Origin | Shandong, China |
| Model | HH.CP-T |
| Capacity | 80 L |
| Temperature Range | RT+5°C to 50°C |
| Temperature Uniformity | ±0.3°C |
| Humidity Range | 20–50% RH (natural evaporation) |
| Heating Method | Air-jacketed |
| CO₂ Control | Gas-mixing (0–20% v/v) |
| CO₂ Recovery Time | ≤1.2 × setpoint (min) |
| Power Input | 450 W |
| Ambient Operating Temp | 5–35°C |
| Inner Chamber Dimensions | 500 × 400 × 400 mm |
| Standard Shelving | 2 stainless steel trays |
| UV Sterilization Lamp | Integrated |
| Door Temperature Control | Yes |
| Dedicated CO₂ Regulator Included | Yes |
Overview
The HeHuo HH.CP-T Carbon Dioxide Incubator is an air-jacketed, microprocessor-controlled environmental chamber engineered for reliable, long-term cultivation of mammalian cells, primary isolates, and sensitive microbial cultures under physiologically relevant conditions. It maintains a tightly regulated atmosphere of 5% CO₂ (adjustable from 0–20% v/v), precise temperature (RT+5°C to 50°C), and stable relative humidity (20–50% RH via natural evaporation), replicating the in vivo extracellular environment essential for maintaining pH homeostasis in bicarbonate-buffered media. Designed for routine use in academic laboratories, biopharmaceutical QC facilities, and contract research organizations, the HH.CP-T adheres to fundamental engineering principles of thermal stability, contamination mitigation, and operational repeatability—critical for GLP-compliant cell passaging, transfection studies, and assay development.
Key Features
- Microprocessor-based temperature controller with timer function and 0.1°C resolution, delivering ±0.3°C uniformity across the 80 L working volume (measured at 37°C, empty chamber, 20°C ambient).
- Air-jacketed heating architecture ensures rapid thermal recovery (<15 min from 25°C to 37°C) and minimizes condensation risk on culture vessel surfaces.
- Electropolished mirror-finish stainless steel inner chamber with fully rounded corners and seamless argon arc welding—optimized for cleaning, autoclave compatibility, and resistance to corrosion from repeated ethanol or hydrogen peroxide decontamination.
- Integrated UV-C germicidal lamp (254 nm) with safety interlock, enabling scheduled sterilization cycles between experiments without chemical residue.
- Dedicated door temperature control circuit actively heats the inner glass door surface to prevent moisture condensation—a critical feature for maintaining optical clarity during microscopy-based monitoring and reducing cross-contamination risk.
- Factory-installed CO₂ regulator and gas-mixing manifold support direct connection to standard medical-grade CO₂ cylinders; no external gas controller required for basic operation.
Sample Compatibility & Compliance
The HH.CP-T accommodates standard T-25, T-75, and 6-/12-/96-well plates, Petri dishes, roller bottles, and flasks up to 500 mL. Its internal geometry (500 × 400 × 400 mm) permits unobstructed airflow and uniform CO₂ distribution when loaded with two included 304 stainless steel shelves. The incubator meets general safety requirements per IEC 61010-1:2010 (Laboratory Equipment) and supports documentation workflows aligned with ISO/IEC 17025 and FDA 21 CFR Part 11 when paired with external data loggers. While not certified for GMP manufacturing environments, its design facilitates audit readiness for cell banking, vaccine production support, and preclinical toxicology labs operating under GLP frameworks.
Software & Data Management
The HH.CP-T operates via embedded firmware with local parameter storage and real-time display of chamber temperature, CO₂ concentration, and elapsed time. It does not include built-in Ethernet/Wi-Fi connectivity or proprietary software. However, analog voltage outputs (0–5 V DC) for temperature and CO₂ are available at the rear panel for integration with third-party SCADA systems, PLCs, or continuous validation platforms (e.g., DeltaTrak, DicksonOne). All operational events—including door openings, UV activation, and alarm triggers—are timestamped in non-volatile memory and retrievable via front-panel navigation, supporting basic traceability requirements for lab notebooks and internal SOPs.
Applications
- Mammalian cell culture maintenance (HEK293, CHO, HeLa, iPSCs) requiring stable 5% CO₂ and 37°C.
- Embryology and assisted reproductive technology (ART) workflows where humidity stability reduces osmotic stress on oocytes and blastocysts.
- Microbial culture of capnophilic organisms (e.g., Neisseria gonorrhoeae, Campylobacter jejuni) under controlled CO₂ enrichment.
- Long-term toxicity screening assays using adherent or suspension cultures under defined atmospheric stressors.
- Biological reagent conditioning (e.g., collagen gels, Matrigel™ thawing) prior to experimental use.
FAQ
Does the HH.CP-T support humidification above 50% RH?
No—the unit relies on passive natural evaporation from a removable water pan and is rated for 20–50% RH at 37°C. For applications requiring >50% RH, supplemental external humidification or alternative models with active steam generation are recommended.
Is the CO₂ sensor calibrated at the factory?
Yes—each unit ships with a zero-point and span calibration certificate for the infrared CO₂ sensor, traceable to NIST standards. Annual recalibration is advised for regulatory continuity.
Can the incubator be validated for IQ/OQ/PQ protocols?
Yes—its mechanical design, sensor layout, and accessible analog outputs allow full qualification per ASTM E2500 and ISO 13485 Annex C when supported by documented procedures and third-party verification tools.
What is the maximum load capacity for shelf weight?
Each stainless steel shelf supports up to 15 kg uniformly distributed; total payload must remain below 30 kg to preserve airflow dynamics and thermal uniformity.
Is the UV lamp ozone-free?
Yes—the installed low-pressure mercury lamp emits exclusively at 254 nm and produces negligible ozone, complying with OSHA PEL limits for laboratory exposure.
