Hesiyi LXL-HR21M 3000 mL Continuous-Flow Titanium Rotor High-Speed Refrigerated Centrifuge

Overview

The Hesiyi LXL-HR21M is a floor-standing, high-speed refrigerated centrifuge engineered for large-volume continuous processing in demanding laboratory and bioprocessing environments. It operates on the principle of continuous-flow sedimentation centrifugation—where sample suspension is introduced continuously into a rotating titanium rotor chamber, enabling real-time separation of suspended particles (e.g., cells, organelles, or viral particles) from clarified supernatant under sustained high gravitational force. Unlike batch-mode centrifuges, this system eliminates repeated loading/unloading cycles, significantly improving throughput for applications such as microbial harvest, cell culture clarification, and biopharmaceutical intermediate concentration. The titanium rotor construction ensures exceptional corrosion resistance, mechanical stability at elevated rotational speeds, and compatibility with aggressive biological buffers and solvents—critical for GMP-aligned process development and scale-down studies.

Key Features

• High-torque brushless AC frequency-controlled motor—eliminates carbon brush wear, reduces maintenance, and ensures long-term rotational stability.
• Intelligent microprocessor-based control system with dual-input interface (touchscreen + physical keys) and real-time graphical display of speed ramping profiles, RCF curves, temperature trends, and integrated centrifugation time.
• Comprehensive safety architecture: electronic door interlock, overspeed protection, rotor imbalance detection, overtemperature shutdown, and automatic rotor recognition via embedded RFID (for certified rotors).
• Programmable operation: up to 16 user-defined protocols; adjustable acceleration/deceleration across 10 ramp levels; customizable hold times and temperature setpoints during run.
• Refrigeration system powered by a high-efficiency Tecumseh (Tai Kang) compressor using R404a refrigerant—compliant with international environmental regulations and delivering precise thermal control within ±1 °C across the full operating range (−20 °C to +40 °C).
• Low-noise design achieved through vibration-damping chassis, food-grade silicone monolithic sealing gasket, and optimized aerodynamic rotor housing geometry.

Sample Compatibility & Compliance

The LXL-HR21M accommodates both batch and continuous-flow configurations. Standard fixed-angle rotors support common labware including 10–500 mL conical tubes (e.g., Beckman Coulter polypropylene bottles), while the optional 3000 mL continuous-flow titanium rotor enables uninterrupted processing of clarified lysates, fermentation broths, or plasma fractions. All rotors are validated per ISO 13485:2016 requirements for medical device manufacturing environments. The system’s firmware supports audit-trail logging (user ID, timestamp, parameter changes, error events), aligning with GLP and FDA 21 CFR Part 11 data integrity expectations when paired with networked data export functionality. Structural materials—including titanium alloy rotor bodies and stainless-steel chamber liners—meet USP Class VI biocompatibility standards for contact with therapeutic intermediates.

Software & Data Management

The onboard control software provides synchronized visualization of operational parameters and historical trend analysis. Run logs—including speed, RCF, temperature, elapsed time, and rotor ID—are automatically timestamped and exportable via USB interface in CSV format. Optional Ethernet connectivity allows integration into centralized laboratory information management systems (LIMS) for remote monitoring and automated report generation. Firmware updates are performed via secure authenticated download, ensuring traceability and version control. All data storage adheres to ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) for regulated quality assurance workflows.

Applications

• Harvesting mammalian, insect, or microbial cells from large-volume bioreactor runs (up to 3 L/h throughput).
• Clarification of crude protein extracts and nucleic acid preparations prior to chromatographic purification.
• Concentration of virus-like particles (VLPs), exosomes, and extracellular vesicles in vaccine and diagnostics R&D.
• Plasma fractionation and platelet-rich plasma (PRP) preparation in transfusion medicine and regenerative therapy labs.
• Downstream processing support in contract development and manufacturing organizations (CDMOs) performing tech transfer of centrifugal unit operations.

FAQ

What is the maximum continuous processing capacity of the titanium rotor?

The 3000 mL continuous-flow titanium rotor supports uninterrupted feed rates up to 3 L/h, depending on sample viscosity and particle settling characteristics.

Is rotor balancing required before each run?

No—automatic imbalance detection and adaptive compensation algorithms eliminate manual balancing for certified rotors; however, symmetrical tube loading remains essential for non-rotor-specific configurations.

Can the system be integrated into a cleanroom environment?

Yes—the sealed refrigeration circuit, low particulate emission design, and smooth stainless-steel surfaces meet ISO Class 7 cleanroom compatibility guidelines when installed with appropriate HVAC support.

Does the instrument support IQ/OQ documentation packages?

Yes—Hesiyi provides vendor-validated installation qualification (IQ) and operational qualification (OQ) templates compliant with ASTM E2500 and EU Annex 15 standards.

What certifications apply to the titanium rotor material?

The Grade 5 (Ti-6Al-4V) titanium alloy meets ASTM F136 specifications for surgical implant applications and is certified for repeated autoclaving at 121 °C, 2 bar for 30 minutes.