HJ HS-840U Vertical Laminar Flow Clean Bench

Overview

The HJ HS-840U Vertical Laminar Flow Clean Bench is an ISO Class 5-certified containment workstation engineered for unidirectional vertical airflow and particulate-free operation in non-hazardous laboratory environments. Unlike horizontal flow benches, the HS-840U directs filtered air vertically downward across the work surface—minimizing turbulence, reducing cross-contamination risk, and ensuring consistent protection of sensitive samples during aseptic procedures. Its design complies with core principles outlined in ISO 14644-1 (Cleanrooms and associated controlled environments), USP (Pharmaceutical Compounding—Sterile Preparations), and EU GMP Annex 1 (Manufacture of Sterile Medicinal Products). The unit employs a single-stage filtration architecture: ambient air is drawn through a durable anodized aluminum pre-filter to capture coarse particulates (>5 µm), then passes through a certified HEPA filter (EN 1822 H14 or equivalent, ≥99.995% efficiency at 0.3 µm) before entering the work area as laminar downflow. This configuration supports routine microbiological handling, cell culture setup, media preparation, and sterile instrument staging under validated cleanroom-equivalent conditions.

Key Features

• Vertical laminar airflow system delivering uniform, low-turbulence downflow across the entire work surface (700 × 560 mm active zone)
• Single HEPA filter (695 × 600 × 38 mm, H14 grade) mounted in a compact, low-profile static pressure chamber—optimized to reduce internal volume and improve energy efficiency
• Dual-speed electronically controlled centrifugal blower with real-time airflow feedback, maintaining stable velocity between 0.25–0.45 m/s under varying filter loading conditions
• Integrated dual-lamp system: 20 W cool-white fluorescent lamp for task illumination (≥300 lux at work surface) and separate 20 W UV-C germicidal lamp (254 nm) for pre-use surface decontamination
• Corrosion-resistant anodized aluminum pre-filter frame and seamless stainless steel or aluminum-plastic composite work surface (non-porous, easy to decontaminate)
• Front-access single-operator configuration with tempered glass side panels and full-height sash for ergonomic positioning and visual monitoring
• Low-noise operation (≤62 dB[A] at 1 m) and minimal mechanical vibration (≤5 µm peak-to-peak in all axes), critical for micromanipulation and long-duration assays

Sample Compatibility & Compliance

The HS-840U is intended for use with non-volatile, non-toxic, non-hazardous biological and pharmaceutical materials—including bacterial cultures, mammalian cell lines, agar plates, pipette tips, vials, and sterile instruments. It is not designed for chemical fume containment or biohazardous aerosol generation (e.g., open-path viral work or mycobacterial culturing). The unit meets ISO 14644-1 Class 5 performance requirements when operated per manufacturer-specified maintenance intervals and validated filter integrity testing (e.g., DOP/PAO scanning per ISO 14644-3). Routine microbial monitoring (settle plate method per ISO 14698-1) confirms compliance with ≤0.5 CFU/plate·hour recovery using 90 mm TSA plates. Documentation supports GLP-compliant lab audits, including traceable calibration records for airflow velocity sensors and UV intensity meters.

Software & Data Management

The HS-840U operates via a dedicated microprocessor-based control panel featuring an LCD interface with tactile membrane switches. While it does not incorporate networked software or cloud connectivity, its embedded controller logs operational parameters—including cumulative runtime, fan speed mode (low/high), UV exposure duration, and fault alerts (e.g., filter saturation warning, lamp failure). All logged data are retained locally and exportable via USB port for integration into laboratory quality management systems (QMS). The control architecture supports 21 CFR Part 11–compliant audit trail generation when paired with optional third-party QMS middleware—enabling electronic signature, user access tiers, and immutable event logging required for FDA-regulated environments.

Applications

• Aseptic transfer and subculturing of bacterial and fungal isolates in clinical microbiology labs
• Preparation of sterile culture media, reagents, and dilution blanks in food safety and environmental testing laboratories
• Staging and assembly of sterile surgical instruments prior to autoclaving or ethylene oxide sterilization
• Handling of primary human keratinocytes and fibroblasts in dermatological research workflows
• Quality control testing of pharmaceutical excipients and finished dosage forms under ISO Class 5 conditions
• Supporting ISO/IEC 17025-accredited calibration labs for particulate reference standard preparation

FAQ

What ISO cleanliness class does the HS-840U achieve, and how is it verified?
The HS-840U achieves ISO Class 5 (equivalent to Fed. Std. 209E Class 100) at ≥0.5 µm particle size. Verification requires periodic airborne particle counting per ISO 14644-1 Annex B and filter integrity testing per ISO 14644-3.
Can this unit be used for handling hazardous chemicals or volatile solvents?
No. The HS-840U provides only particulate filtration—not vapor or gas containment. Chemical fume hoods or ductless recirculating units with activated carbon filtration are required for such applications.
How often must the HEPA filter be replaced, and what documentation is provided?
Filter replacement interval depends on usage intensity and ambient air quality; typical service life is 18–24 months. Each unit ships with a Certificate of Conformance, HEPA filter test report (including penetration efficiency and initial resistance), and preventive maintenance checklist.
Is UV lamp intensity monitored or calibrated during operation?
UV output is not continuously monitored but is validated during installation and annually thereafter using a NIST-traceable UV radiometer. Intensity must exceed 40 µW/cm² at 1 m distance to ensure effective surface decontamination per ISO 15714.
Does the unit support integration with building management systems (BMS) or LabVantage/LIMS platforms?
Native BMS integration is not supported; however, analog 0–10 V or digital Modbus RTU outputs can be added via optional I/O module for status monitoring and alarm relay signaling.