Huiron HRH-DSA350 High-Flow Bioaerosol Sampler

Overview

The Huiron HRH-DSA350 High-Flow Bioaerosol Sampler is an engineered field-deployable instrument designed for quantitative collection of airborne biological particles—including bacteria, viruses, fungal spores, and bacterial endospores—from ambient or indoor air environments. It operates on the principle of electrostatic filtration, where charged particles are captured with high efficiency on a conductive filter medium under controlled laminar airflow. Unlike impaction or impinger-based systems, this method preserves particle viability and structural integrity—critical for downstream culture-based assays, PCR amplification, metagenomic sequencing, and immunoassay analysis. With a programmable flow range of 100–300 L/min, the HRH-DSA350 achieves rapid volumetric sampling (e.g., 1,800 L in 6 minutes at 300 L/min), enabling statistically robust detection of low-concentration bioaerosols in large-volume spaces such as transportation hubs, healthcare facilities, pharmaceutical cleanrooms, livestock barns, and border inspection checkpoints.

Key Features

• High-flow electrostatic capture: Optimized aerodynamic design ensures stable, pulse-free airflow across the full operational range; maintains ≥95% collection efficiency for particles ≥2 µm, ≥75% for 0.5–1 µm, and ≥70% for submicron particles ≤0.5 µm—validated per GB/T 39990–2021.
• Integrated on-site elution: Paired with a dedicated elution module, the sampler enables complete transfer of captured bioparticles from the electrostatic filter into ≤5 mL of liquid medium within ≤60 seconds—minimizing handling loss and preserving sample integrity prior to lab analysis.
• All-weather operational resilience: Certified for continuous operation between −40 °C and +70 °C; aluminum alloy enclosure meets IP65 ingress protection and EMI shielding requirements for use in electrically noisy or humid outdoor environments.
• Extended autonomous runtime: Rechargeable lithium-ion battery supports >30 hours of uninterrupted sampling at maximum flow (300 L/min); battery status, remaining runtime, and thermal management are continuously monitored and reported via embedded firmware.
• Multi-mode triggering & control: Supports manual initiation, time-scheduled cycles, hardware TTL-triggered acquisition (e.g., synchronized with environmental sensors), and remote software command via Bluetooth or Wi-Fi—enabling integration into automated monitoring networks.
• Low acoustic signature: Acoustic noise ≤60 dB(A) at 1 m distance ensures non-disruptive deployment in sensitive locations including hospitals, schools, and office buildings.

Sample Compatibility & Compliance

The HRH-DSA350 is validated for compatibility with standard microbiological and molecular workflows. Collected eluates are directly compatible with ISO 14698-1:2003 (biocontamination control), USP , and ASTM D6329-17 (microbial air sampling). Its electrostatic filter substrate is inert toward common eluents (e.g., PBS, saline, glycine buffer) and does not interfere with DNA/RNA extraction kits or culture media formulations. The device complies fully with GB/T 39990–2021 “Technical Specifications for Particulate Bioaerosol Samplers”, and its data logging architecture supports audit-ready timestamped records required under GLP and GMP-aligned environmental monitoring programs. Firmware includes configurable user authentication and event-logged parameter changes to satisfy basic traceability needs in regulated settings.

Software & Data Management

The bundled Huiron BioSampler Control Suite (Windows/macOS) provides full configuration and real-time telemetry via USB-serial (DB-9) or wireless link. Users can define up to 99 independent sampling protocols—including flow rate, total volume, cycle duration/interval, start delay, repeat count, and device ID. All parameters are stored in non-volatile memory with CRC-32 validation. Software exports time-stamped CSV logs containing flow rate, battery voltage, temperature, pump duty cycle, and error flags. Exported datasets include UTC timestamps synchronized to system clock (±1 s accuracy), supporting temporal correlation with meteorological or occupancy data. No cloud upload or telemetry transmission occurs unless explicitly enabled by user—ensuring local data sovereignty and compliance with institutional IT policies.

Applications

• Real-time surveillance of airborne pathogens in public transit infrastructure (airports, rail stations, bus terminals)
• Environmental monitoring in Grade A/B cleanrooms per ISO 14644-1 and EU GMP Annex 1
• Outbreak investigation support in hospitals and CDC field laboratories
• Validation of HVAC filtration efficacy and room air exchange rates
• Frontline biosurveillance at ports of entry, mail screening facilities, and military CBRN response units
• Long-term ecological studies of atmospheric microbial communities in agricultural and urban settings

FAQ

What types of biological agents can the HRH-DSA350 effectively collect?
It captures viable and non-viable particulates including vegetative bacteria, enveloped/non-enveloped viruses, fungal conidia and hyphal fragments, pollen, and bacterial endospores—provided they remain airborne and within the defined aerodynamic size range.
Is the electrostatic filter reusable?
No. Each filter is single-use and sterilized pre-installation; reuse compromises collection efficiency and introduces cross-contamination risk.
Can the eluate be used directly for qPCR without concentration?
Yes—when sampling at ≥300 L/min for ≥5 minutes, typical eluate concentrations meet LOD requirements for most clinical and environmental qPCR assays targeting influenza, SARS-CoV-2, Aspergillus, or Bacillus anthracis markers.
Does the system support regulatory-compliant data archiving?
While not FDA 21 CFR Part 11-certified out-of-the-box, the software generates immutable, time-stamped CSV logs suitable for inclusion in ALCOA+ compliant documentation packages when paired with organizational SOPs for electronic record retention.
How is calibration verified?
Flow calibration is performed at factory using NIST-traceable mass flow meters; users receive a certificate of conformance. Field verification may be conducted using a primary calibrator (e.g., Gilian Gilibrator-2) connected upstream of the inlet per ISO 17025 guidelines.