Huironghe HRH-866 Programmable Controlled-Rate Freezing Container
| Brand | Huironghe |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | HRH-866 |
| Quotation | Available Upon Request |
| Material | Ultra-Low-Temperature-Resistant Polyethylene (PE) |
| Cooling Rate | 1 °C/min (programmable) |
| Capacity | 7 × 1.0–2.0 mL Cryovials |
| Dimensions (Ø × H) | 93 × 90 mm |
Overview
The Huironghe HRH-866 Programmable Controlled-Rate Freezing Container is a passive, non-electric cryopreservation device engineered for reproducible, slow-rate freezing of sensitive biological samples—particularly mammalian cells—without reliance on external power or complex instrumentation. It operates on the principle of controlled thermal conduction through phase-change material (PCM)-enhanced insulation and calibrated heat dissipation geometry. Unlike active programmable freezers, the HRH-866 achieves consistent −1 °C/min cooling by leveraging its precisely engineered PE housing, optimized wall thickness, and internal thermal mass distribution. This rate aligns with empirically validated cryobiological protocols for minimizing intracellular ice formation and osmotic shock during vitrification-inhibited freezing. The device is intended for use in standard −80 °C mechanical freezers or liquid nitrogen vapor-phase storage environments, serving as a cost-effective, maintenance-free alternative to benchtop controlled-rate freezers in academic core facilities, biobanks, and QC laboratories.
Key Features
- Passive, electricity-free operation—no software, no calibration drift, no consumables beyond standard cryogenic media
- Consistent −1 °C/min cooling profile verified across multiple freeze cycles and ambient freezer temperature variations (−70 °C to −85 °C)
- Ultra-low-temperature-resistant polyethylene construction—tested for structural integrity and chemical inertness at −196 °C (liquid nitrogen immersion)
- Compact cylindrical form factor (Ø93 × 90 mm) designed for efficient stacking and compatibility with standard freezer racks and cryoboxes
- Seven-position vial holder accommodates 1.0–2.0 mL externally threaded cryovials (e.g., Nunc, Corning, Thermo Fisher formats) with positive retention and minimal positional shift during handling
- Autoclavable at 121 °C (steam sterilization compatible) for reuse in GLP-compliant cell banking workflows
Sample Compatibility & Compliance
The HRH-866 is validated for cryopreservation of primary human and rodent cells, induced pluripotent stem cells (iPSCs), mesenchymal stromal cells (MSCs), hybridomas, and adherent and suspension cell lines—including CHO, HEK293, and Jurkat. Its standardized cooling kinetics support adherence to ISO 20387:2018 (Biobanking — General requirements for biobanking) and complement internal SOPs aligned with ISCT (International Society for Cell & Gene Therapy) minimum criteria for MSC cryostorage. While not an active medical device, its performance characteristics are referenced in method validation documentation for USP Sterility Tests and FDA guidance on cell therapy product stability assessment. The PE material complies with USP Class VI biological reactivity standards and exhibits negligible leachables under cryogenic stress per ASTM F2459-22 (Standard Guide for Extractables Testing of Medical Devices).
Software & Data Management
The HRH-866 requires no embedded firmware, drivers, or proprietary software. Its operational parameters are fixed by physical design—not user-programmable—ensuring protocol fidelity and eliminating version control or cybersecurity concerns associated with connected instruments. All usage records (e.g., lot number, freeze date, operator ID, sample IDs) must be manually logged in laboratory notebooks or integrated into LIMS via barcode/RFID-enabled cryovial labeling. For audit readiness, users may document freeze validation data—including thermocouple traces from representative runs using calibrated loggers (e.g., Vaisala, Omega)—to demonstrate compliance with internal QA checklists and 21 CFR Part 11–aligned electronic record practices where applicable.
Applications
- Routine cryopreservation of clinical-grade cell products prior to release testing
- Long-term archival storage of primary isolates in translational research cohorts
- Standardized freezing for inter-laboratory reproducibility studies (e.g., NIH Common Fund Human Biomolecular Atlas Program)
- Backup freezing strategy during controlled-rate freezer downtime or maintenance windows
- Training tool for cryobiology fundamentals in undergraduate and graduate teaching laboratories
- Pre-freeze conditioning of organoids and spheroids requiring gradual thermal transition
FAQ
Is the HRH-866 suitable for freezing sperm or oocytes?
No—its −1 °C/min profile is optimized for somatic and stem cells; gamete cryopreservation typically requires faster or slower rates (e.g., −15 °C/min for sperm, −0.3 °C/min for oocytes) and specialized carriers.
Can it be used directly in liquid nitrogen?
Yes—the PE housing is rated for direct immersion in liquid nitrogen (−196 °C); however, rapid thermal shock may occur if transferred directly from room temperature; pre-chilling in dry ice or −80 °C is recommended.
Does it require pre-cooling before use?
Yes—equilibrate the empty unit at −20 °C for ≥30 minutes prior to loading samples to minimize thermal lag and ensure profile consistency.
How many freeze-thaw cycles can it withstand?
Accelerated aging tests indicate >200 full cycles (−80 °C ↔ 25 °C) without measurable deformation or loss of cooling fidelity when handled per manufacturer instructions.
