HuRongHe HRH-BSA300X High-Flow Wet-Wall Cyclonic Aerosol Bio-Sampler

Overview

The HuRongHe HRH-BSA300X is a high-flow, wet-wall cyclonic aerosol bio-sampler engineered for quantitative recovery of viable and non-viable biological particles—including bacteria, fungi, viruses, and spores—from large volumes of ambient or controlled-environment air. It operates on the principle of inertial impaction combined with continuous liquid-film generation inside a rotating wet-wall cyclone chamber. Airborne particles are accelerated tangentially into the cyclone; under centrifugal force, they impact a dynamically maintained aqueous film lining the inner wall surface. This mechanism ensures high-efficiency particle capture (>90% for particles ≥0.5 µm aerodynamic diameter), minimal shear-induced damage to fragile microorganisms, and direct elution into a sterile collection medium—enabling downstream molecular analysis (e.g., qPCR, digital PCR, metagenomic sequencing) without additional extraction steps.

Key Features

• High-volume sampling capability with programmable flow rates up to 300 L/min, enabling representative environmental monitoring in cleanrooms, pharmaceutical manufacturing suites, hospitals, and biosafety laboratories.
• Self-regulating wet-wall cyclone architecture maintains a stable liquid film (0.1–1 mm thickness) across ≥200 cm² contact area, ensuring consistent particle deposition efficiency across variable humidity and temperature conditions (0–46 °C).
• Integrated reservoir system (≥500 mL capacity) supports automatic top-up of evaporative losses during extended sampling, eliminating manual intervention and maintaining constant liquid volume and pH stability.
• Dual-stage fluid management: primary cyclonic capture followed by high-speed (≥100 rpm) droplet separation via a rotating baffle rotor, minimizing cross-contamination between sampling cycles.
• Configurable operational modes—including manual trigger, IR remote control, time-scheduled auto-start, and Ethernet/Wi-Fi-enabled remote command—support integration into facility-wide environmental monitoring systems (EMS).
• Automated post-sampling protocols include configurable rinse cycles (up to 3x), internal line flushing, and scheduled maintenance routines (e.g., pump calibration, sensor diagnostics) logged with timestamped audit trails.
• Rugged, portable design (≤8 kg net weight; max. 745×550×240 mm footprint) facilitates deployment in mobile labs, field epidemiology units, and multi-point surveillance networks.

Sample Compatibility & Compliance

The HRH-BSA300X is validated for use with standard microbiological collection media including PBS, saline, and proprietary viral transport media (VTM). Its wet-collection output is compatible with ISO 14698-1:2003 (Biocontamination control — Part 1: General principles and methods) and ISO 14698-2:2003 (Sampling of bioaerosols), as well as USP “Microbiological Control and Monitoring of Aseptic Processing Environments.” The device’s materials of construction (316L stainless steel wetted surfaces, medical-grade silicone tubing, PTFE-coated rotors) withstand repeated sterilization via vaporized hydrogen peroxide (VHP) at concentrations up to 1200 ppm and immersion in 5% sodium hypochlorite solution—meeting CDC-recommended decontamination guidelines for high-consequence pathogen sampling equipment.

Software & Data Management

Embedded firmware supports local parameter configuration via OLED touchscreen interface and full remote supervision via HTTPS-secured web dashboard. All sampling events—including start/stop timestamps, flow rate logs, battery status, liquid level telemetry, and error codes—are stored internally with SHA-256 hashing and exportable in CSV/JSON formats. Audit trail functionality complies with FDA 21 CFR Part 11 requirements for electronic records and signatures when deployed in GMP-regulated environments. Optional API integration enables synchronization with LIMS (e.g., LabWare, STARLIMS) and enterprise EMS platforms.

Applications

• Real-time bioaerosol surveillance in Grade A/B cleanrooms during aseptic drug manufacturing.
• Outbreak investigation support in healthcare facilities—rapid acquisition of nucleic-acid-amenable samples from airborne transmission zones.
• Validation of HVAC filtration efficacy and room air exchange rates per ISO 14644-1 and EU GMP Annex 1.
• Environmental baseline studies for biodefense and pandemic preparedness programs.
• Research on airborne microbial community dynamics in indoor/outdoor urban and occupational settings.

FAQ

What types of microorganisms can be effectively recovered using the HRH-BSA300X?

It achieves high recovery efficiency for vegetative bacteria (e.g., Bacillus subtilis, Pseudomonas aeruginosa), fungal conidia (e.g., Aspergillus niger), enveloped and non-enveloped viruses (e.g., MS2, Phi6), and bacterial spores—validated per ISO 14698-2 Annex B.
Is the device suitable for unattended overnight sampling?

Yes. With ≥6 h battery autonomy and programmable 6-hour maximum runtime, it supports fully autonomous operation; optional AC adapter enables indefinite continuous use.
How does the system prevent carryover between sequential samples?

Automated post-sampling rinse cycles—using fresh collection medium—and physical separation of aerosol path from liquid reservoir ensure negligible carryover (<0.01% per ISO 14698-2 validation data).
Can sampling parameters be synchronized across multiple units in a networked deployment?

Yes. Time-synchronized sampling initiation, centralized firmware updates, and aggregated data ingestion are supported via DHCP-assigned IP addressing and NTP time alignment.
Does the unit meet regulatory requirements for use in GMP manufacturing environments?

It is designed to support compliance with EU GMP Annex 1 (2022), PIC/S PI 036-6, and FDA guidance on environmental monitoring; full IQ/OQ documentation packages are available upon request.