JEIO TECH IST-3075/3075R & IST-4075/4075R Benchtop Incubated Shaker

Overview

The JEIO TECH IST Series Benchtop Incubated Shaker integrates precise temperature-controlled incubation with uniform orbital shaking in a compact, laboratory-grade platform. Engineered for reproducible cell culture, microbial growth, enzyme kinetics, solubility studies, and biochemical assays, the IST system employs a microprocessor-driven PID temperature controller coupled with a brushless DC (BLDC) motor to deliver stable thermal environments and highly repeatable shaking motion. Unlike conventional shakers with ambient-only operation, the IST series supports both heated-only (IST-3075/4075) and refrigerated-heated (IST-3075R/4075R) configurations—enabling experiments across physiologically relevant ranges from +15°C to 80°C (refrigerated models) or ambient +5°C to 80°C (non-refrigerated). The 19 mm orbital diameter ensures gentle yet effective mixing for suspension cultures and viscous media, while the BLDC motor’s torque consistency maintains speed accuracy under variable load conditions—including multi-flask configurations or uneven weight distribution.

Key Features

• Intuitive 7-inch color touchscreen LCD interface with icon-based navigation, logical menu hierarchy, and non-volatile memory—ensuring automatic resumption of setpoints after power interruption.
• Intelligent shaking control: real-time speed deviation monitoring triggers audible/visible alarms; automatic speed adjustment compensates for unbalanced loads, floor instability, or external mechanical shock.
• Three-point temperature calibration and auto-tuning PID algorithm guarantee ±0.3°C temperature stability and ≤±0.5°C uniformity across the chamber (measured at 37°C, 100 rpm).
• Stainless steel interior chamber with seamless welds and corrosion-resistant finish, compatible with routine sterilization protocols and aqueous/organic solvent exposure.
• Triple-side transparent acrylic lid with integrated bright-white LED lighting enables continuous visual monitoring without thermal leakage or CO₂ loss—critical for long-term aerobic cultures.
• Gas-spring assisted lid mechanism ensures smooth, controlled opening/closing and eliminates operator fatigue during frequent sample access.
• Three-step adjustable high-velocity circulation fan optimizes thermal recovery (<3 min to re-stabilize after 10-sec door opening at 37°C) and minimizes vertical/horizontal temperature gradients.
• Refrigerated models utilize environmentally compliant R134a refrigerant (CFC-free) and feature mechanical over-temperature cut-off independent of electronic controls.

Sample Compatibility & Compliance

The IST platform accommodates standard laboratory vessels including Erlenmeyer flasks (up to 5 L), test tubes, deep-well plates, and custom trays via interchangeable clamps and universal platforms. Its sealed silicone gasket and air-tight door design maintain internal environmental integrity—supporting applications requiring stable humidity retention (when used with optional water pan) and minimal cross-contamination risk. The system complies with IEC 61010-1:2010 for electrical safety in laboratory equipment and meets electromagnetic compatibility (EMC) requirements per EN 61326-1. While not certified for GLP/GMP production environments, its audit-ready data logging (via RS-232/USB), parameter traceability, and alarm event timestamps align with ISO/IEC 17025 documentation expectations for accredited testing laboratories.

Software & Data Management

Data acquisition is supported through native RS-232 serial communication (ASCII protocol) and USB 2.0 host interface, enabling integration with LabVIEW, MATLAB, or custom SCADA systems. Real-time parameters—including actual/shake speed, chamber temperature, elapsed time, and alarm status—are streamable at user-defined intervals (1–60 sec). All setpoints, runtime logs, and fault events are stored in non-volatile memory with timestamping (year/month/day/hour/min/sec). Optional PC software (JEIO TECH IST Control Suite v3.2+) provides graphical trend visualization, CSV export, and password-protected configuration locking—facilitating compliance with FDA 21 CFR Part 11 requirements when deployed with validated IT infrastructure.

Applications

• Mammalian and insect cell suspension culture under controlled O₂/temperature conditions
• Microbial fermentation optimization (E. coli, yeast, bacilli) with parallel condition screening
• Protein expression and purification workflows requiring consistent agitation during induction
• Solubility and dissolution rate studies per USP and
• Enzyme activity assays where thermal stability and mixing homogeneity directly impact kinetic reproducibility
• Antibiotic susceptibility testing using broth microdilution with automated sampling interfaces
• Environmental microbiology—e.g., BOD incubation with simultaneous aeration and temperature regulation

FAQ

What is the maximum flask capacity supported on the IST-4075 platform?
The IST-4075 accepts up to four 2 L Erlenmeyer flasks or two 5 L flasks using the heavy-duty dual-clamp adapter (sold separately). Platform load capacity is rated at 15 kg max.
Does the IST series support CO₂-enriched incubation?
No—this model lacks gas inlet/outlet ports and humidification control; it is designed for air-based incubation only. For CO₂ applications, consider JEIO TECH’s CCI series CO₂ incubators.
Can the shaking orbit be changed from 19 mm to another diameter?
No—the 19 mm orbital path is fixed by mechanical design and cannot be modified. Alternative orbit sizes require different shaker platforms (e.g., 25 mm on JEIO TECH’s IS-RD series).
Is firmware update capability available via USB?
Yes—firmware updates are distributed as .bin files via JEIO TECH’s official support portal and installed using the USB port and on-screen bootloader utility.
How is temperature uniformity validated across the chamber?
JEIO TECH provides factory calibration reports per ISO/IEC 17025-accredited procedures, documenting measurements at nine spatial points (3×3 grid) at 25°C, 37°C, and 60°C under static and shaking conditions (100 rpm).