Junyi JY-CX2A Horizontal Electrophoresis Chamber
| Brand | Junyi |
|---|---|
| Origin | Beijing, China |
| Model | JY-CX2A |
| Gel Tray Dimensions (W×L) | 350×300 mm |
| Sample Capacity | 68 wells (0.4 mm gel thickness, shark-tooth comb) |
| Buffer Volume | ~1500 mL |
| External Dimensions (L×W×H) | 406×176×400 mm |
| Net Weight | 11.2 kg |
| Gel Thickness | 0.4 mm |
| Number of Gels | 1 |
| Comb Type | Removable red shark-tooth comb |
| Safety Features | Lid-activated power cutoff, ergonomic safety release button, detachable electrode assembly |
| Cooling | Passive buffer-based thermal stabilization |
Overview
The Junyi JY-CX2A Horizontal Electrophoresis Chamber is an engineered platform for high-resolution nucleic acid separation in molecular biology laboratories. Designed around the principles of constant-field horizontal electrophoresis, it supports standard agarose and polyacrylamide gel formats under controlled thermal and electrical conditions. Its structural architecture prioritizes reproducibility, operator safety, and experimental flexibility—particularly for applications requiring extended run times or elevated field strengths. Unlike vertical systems, the JY-CX2A’s horizontal configuration enables uniform buffer distribution across the gel surface, minimizing edge effects and improving band sharpness. The chamber integrates passive thermal management via optimized buffer volume and flow geometry, reducing localized heating during high-voltage operation without requiring external chillers or recirculation pumps.
Key Features
- Passive buffer-cooling design ensures consistent temperature distribution across the gel matrix, critical for maintaining DNA conformation integrity and migration linearity during prolonged electrophoresis.
- Lid-activated automatic power cutoff circuit interrupts current flow upon cover opening—complying with IEC 61010-1 safety requirements for laboratory electrical equipment.
- Ergonomic safety release button facilitates one-handed lid operation while preventing accidental displacement during sample loading or buffer adjustment.
- Modular electrode assembly is fully detachable, enabling rapid cleaning, replacement, or calibration without disassembling the main chamber body—reducing downtime and contamination risk.
- Optically enhanced red shark-tooth comb improves visual contrast during sample loading; its precise tooth geometry minimizes well deformation and cross-well diffusion (sample carryover), especially at 0.4 mm gel thickness.
- Integrated drain port located at the base allows complete, residue-free buffer evacuation—supporting GLP-aligned cleaning validation protocols and minimizing cross-run carryover.
Sample Compatibility & Compliance
The JY-CX2A accommodates standard 350 × 300 mm gels at 0.4 mm thickness, supporting both standard (68-well) and high-density (100-well, optional comb) configurations. It is validated for use with conventional TAE and TBE buffers, as well as low-conductivity media required for high-resolution SSCP and heteroduplex analysis. The system meets essential electrical safety standards per IEC 61010-1 and is compatible with regulated workflows under ISO/IEC 17025-accredited laboratories. While not inherently compliant with FDA 21 CFR Part 11, its operational transparency—combined with manual logbook documentation and traceable buffer lot records—supports audit readiness for QA/QC environments conducting AFLP, SSR, or RNase protection assays.
Software & Data Management
The JY-CX2A operates as a standalone electrophoresis platform without embedded firmware or digital control interfaces. As such, it requires external DC power supply units (e.g., 0–300 V, 0–500 mA) with adjustable voltage/current limiting—enabling precise method replication across instruments. Experimental parameters—including applied voltage, run duration, buffer composition, and ambient temperature—are manually recorded to support traceability. When paired with certified gel documentation systems (e.g., UV transilluminators with calibrated CCD imaging), the chamber contributes to full analytical data chains suitable for ISO 15189 or CLIA-compliant reporting frameworks.
Applications
- AFLP (Amplified Fragment Length Polymorphism) profiling for plant and microbial genotyping
- Microsatellite (SSR) marker analysis in population genetics and forensic botany
- SSCP (Single-Strand Conformation Polymorphism) screening for point mutation detection in clinical research settings
- Heteroduplex analysis of mismatched DNA hybrids in recombination studies
- DNA footprinting assays assessing protein-DNA binding specificity
- RNase protection and S1 nuclease mapping for transcript boundary determination
- Primer extension analysis for quantifying transcription start sites and splice variants
FAQ
Does the JY-CX2A include a built-in power supply?
No. It is a passive electrophoresis chamber requiring an external DC power supply capable of delivering stable voltage and current output.
Is the red shark-tooth comb autoclavable?
Yes—the comb is constructed from heat-resistant polycarbonate and withstands standard autoclaving cycles (121°C, 20 min) without warping or discoloration.
Can the chamber accommodate thicker gels (e.g., 1.0 mm)?
No. It is mechanically optimized for 0.4 mm gels only; deviation may compromise sealing integrity and buffer containment.
What buffer volumes are recommended for optimal performance?
A minimum of 1200 mL and maximum of 1600 mL ensure adequate thermal mass and electrode submersion while maintaining laminar buffer flow over the gel surface.
How is electrical grounding implemented?
The chamber features dual grounding terminals on the electrode assembly, compatible with standard laboratory grounding rods or facility earth points per IEC 60364-5-54.
