KDS Legato 130 Syringe Pump
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | Legato 130 |
| Flow Rate | 3.66 pL/min – 3.818 mL/min |
| Accuracy | ±0.5% |
| Repeatability | ±0.05% |
| Syringe Volume Range | 0.5 µL – 1000 µL |
| Display | 4.3″ WQVGA TFT Color Touchscreen |
| Motor | 1.8° Stepper Motor with 1/16 Microstepping |
| Max Linear Force | 5 kg (11 lb) |
| Pusher Travel Rate | 0.433 µm/min – 228.97 mm/min |
| Power Input | 12–30 VDC |
| Dimensions | 22.6 × 17.78 × 9.32 cm |
| Weight | 1.96 kg |
| Operating Temperature | 4–40 °C |
| Storage Temperature | −10–70 °C |
| Humidity | 20–80% RH (non-condensing) |
| Certifications | CE, RoHS |
Overview
The KDS Legato 130 Syringe Pump is a high-precision, micro-volume fluid delivery system engineered for reproducible and programmable infusion and withdrawal in preclinical neuroscience, pharmacology, and behavioral research. Designed around a closed-loop stepper motor architecture with 1/16 microstepping resolution and a precision lead-screw drive mechanism, the Legato 130 delivers exceptional volumetric control across an ultra-broad flow range—from picoliter-per-minute rates required for intracerebral microinjection to milliliter-per-minute delivery for systemic or IV applications. Its direct syringe actuation eliminates compliance-related errors inherent in tubing-based systems, ensuring traceable, linear displacement correlated precisely to commanded volume. The pump operates under constant-force conditions up to 5 kg (11 lb), enabling reliable performance with high-viscosity agents and narrow-bore glass or stainless-steel syringes commonly used in stereotaxic surgery.
Key Features
- 4.3-inch WQVGA color TFT touchscreen interface with intuitive icon-driven navigation—no external PC required for basic operation
- Dual-mode operation: continuous infusion and bidirectional withdrawal (retract mode) for precise dosing and sample recovery
- Non-volatile memory retains all user-defined protocols, syringe calibrations, and system settings after power cycling
- Modular I/O architecture: RS-485, IEEE-1394 (FireWire), USB-B (6-pin), 15-pin D-Sub TTL I/O, and mini-phono footswitch input for hands-free triggering
- Compliant with IEC 61000-6-2 (immunity) and IEC 61000-6-4 (emissions); certified to CE and EU RoHS directives
- Robust mechanical design: aluminum chassis, low-noise stepper drive, and thermal management optimized for extended unattended operation (e.g., overnight infusions)
Sample Compatibility & Compliance
The Legato 130 accommodates standard Luer-lock and small-volume glass syringes ranging from 0.5 µL to 1000 µL—including Hamilton, SGE, Nanofil, and compatible domestic microsyringes. Its force-limited pusher head prevents syringe barrel deformation during high-pressure delivery, preserving calibration integrity over repeated use. While not classified as a medical device under FDA 21 CFR Part 820, the system supports GLP-compliant workflows through audit-trail-capable software integration (via optional KDS Legacy Software or third-party LabVIEW/Python APIs). All firmware and configuration logs are timestamped and exportable in CSV format, facilitating documentation for institutional animal care and use committee (IACUC) reporting and ISO/IEC 17025-aligned laboratory accreditation.
Software & Data Management
The pump supports both standalone and networked operation. Through its USB-B or RS-485 interface, it integrates seamlessly with KDS’s legacy Windows-based control suite (v3.0+), which enables multi-pump synchronization, gradient profile programming, and real-time flow monitoring with data logging at configurable intervals. For automated platforms, open-command protocols (ASCII-based SCPI-like syntax) allow integration into MATLAB, Python (pySerial), or National Instruments LabVIEW environments. All parameter changes—including flow rate, volume, direction, and acceleration—are logged with timestamps and user identifiers when connected to a host system, satisfying traceability requirements for regulated preclinical studies.
Applications
- Stereotaxic microinjection into rodent brain regions (e.g., hippocampus, striatum, ventricles) using NanoFil or Hamilton 7000 series syringes
- Chronic intrathecal or intracerebroventricular (ICV) infusion in longitudinal pharmacokinetic or neurodegeneration models
- Controlled delivery of viral vectors (AAV, lentivirus), siRNA, or CRISPR-Cas9 complexes with sub-nanoliter precision
- Calibration and validation of microfluidic devices requiring stable, low-flow-rate sources
- Automated perfusion in ex vivo tissue slice preparations or organ-on-chip assays
FAQ
What syringe sizes are supported?
The Legato 130 is calibrated for syringes from 0.5 µL to 1000 µL; optimal accuracy is achieved when the selected syringe volume falls within 10–90% of its nominal capacity.
Does the pump support programmable ramping or multi-step protocols?
Yes—via KDS Legacy Software or ASCII command interface, users can define up to 99 sequential steps with independent flow rates, volumes, dwell times, and direction toggling.
Is the system compatible with sterile or laminar-flow hood environments?
The unit’s sealed front panel, absence of ventilation grilles, and low-outgassing housing materials make it suitable for Class II biosafety cabinets; however, full sterilization (e.g., autoclaving) is not supported.
Can multiple Legato 130 units be synchronized?
Yes—using RS-485 daisy-chaining or USB-hub configurations with KDS software, up to 32 pumps can operate in coordinated master-slave mode with sub-millisecond timing alignment.
What maintenance is required?
No routine lubrication or calibration is needed; periodic cleaning of the syringe clamp and pusher shaft with isopropyl alcohol is recommended. Firmware updates are delivered via USB flash drive and retain all stored protocols.
