LB-211B Langbo Large-Capacity Air-Bath Constant-Temperature Orbital Shaker
| Brand | Langbo |
|---|---|
| Origin | Jiangsu, China |
| Model | LB-211B |
| Instrument Type | Air-Bath Constant-Temperature Orbital Shaker |
| Timer Range | 1–999 min |
| Oscillation Frequency | 50–250 rpm |
| Oscillation Amplitude | 30 mm |
| Temperature Range | 4–60 °C |
| Platform Quantity | 1 |
| Capacity | 250 mL × 40 or 2000 mL × 6 (standard configuration) |
Overview
The LB-211B Langbo Large-Capacity Air-Bath Constant-Temperature Orbital Shaker integrates precise temperature control with uniform orbital agitation in a single compact unit. Engineered for high-throughput microbial culture, cell suspension maintenance, solubility studies, and biochemical reaction optimization, it operates on the principle of air-bath convection heating coupled with brushless DC motor-driven orbital motion (25 mm nominal radius, 30 mm peak-to-peak displacement). Unlike water-jacketed systems, its forced-air thermal architecture ensures rapid temperature equilibration, minimal gradient deviation (< ±0.3 °C at 37 °C), and contamination-free operation—critical for sterile applications in microbiology, molecular biology, and bioprocess development labs.
Key Features
- Integrated air-bath thermostatic chamber and orbital shaker platform—eliminates need for separate incubator and shaker, reducing footprint by up to 40% and minimizing cross-contamination risk.
- Robust construction: cold-rolled carbon steel outer casing with electrophoretic coating; 304 stainless steel inner chamber; tempered glass observation window with integrated LED illumination for real-time monitoring without door opening.
- Human-centered tilted control panel with backlit LCD display (128 × 64 pixels), supporting simultaneous readout of setpoint/actual temperature, speed, and remaining time.
- Intelligent parameter retention: all user-defined settings (temperature, speed, timer) are preserved during power interruption and automatically restored upon reboot—compliant with GLP operational continuity requirements.
- Multi-level safety system: over-temperature cutoff (±2 °C hysteresis), motor overload protection, door-open detection, and audible/visual alarm activation for fault conditions.
- High-reliability drive train: imported brushless DC motor with closed-loop speed feedback, delivering stable rotational output across full 50–250 rpm range with ≤±1 rpm deviation under load.
Sample Compatibility & Compliance
The LB-211B accommodates standard laboratory vessels including Erlenmeyer flasks (250 mL × 40 positions), baffled bottles (500–2000 mL), deep-well plates, and custom bioreactor sleeves. Its 30 mm amplitude and orbital motion profile ensure homogeneous suspension of aerobic cultures (e.g., E. coli, S. cerevisiae) and low-shear handling of sensitive mammalian cells. The unit meets IEC 61010-1:2010 safety standards for laboratory equipment and is designed to support ISO/IEC 17025-compliant method validation workflows. Optional accessories—including FDA 21 CFR Part 11–enabled data loggers with audit-trail-capable software—facilitate GMP-aligned documentation in QC/QA environments.
Software & Data Management
While the base model operates via standalone keypad interface, optional RS-485 or USB-C connectivity enables integration with LabVantage, Empower, or custom LIMS platforms. The optional temperature data logger records timestamped values at user-selectable intervals (1–60 min), stores ≥30 days of continuous data onboard, and exports CSV files compatible with JMP, MATLAB, or Python-based analysis pipelines. All firmware updates are delivered via signed binary packages to ensure integrity and traceability—supporting ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).
Applications
- High-density bacterial and yeast cultivation for protein expression and plasmid amplification
- Oxygen transfer optimization in aerobic fermentation processes
- Antibiotic susceptibility testing using broth microdilution protocols (CLSI M07-A11)
- Suspension culture of insect and mammalian cells (e.g., Sf9, CHO-K1) under controlled shear conditions
- Extraction kinetics studies (e.g., phytochemicals, nucleic acids) requiring simultaneous thermal and mechanical energy input
- Environmental sample enrichment (e.g., wastewater microbiota, soil eluates) per EPA Method 1681
FAQ
Is the LB-211B suitable for use with sealed vessels under positive pressure?
Yes—its reinforced chamber seal and pressure-relief vent design allow safe operation with capped flasks and serum bottles at ambient atmospheric equilibrium. For pressurized bioreactors, external pressure regulation is required.
Can the shaker maintain temperature stability during extended overnight runs?
Yes—the PID-controlled heating system, combined with high-density insulation and adaptive duty-cycle modulation, sustains ±0.5 °C stability over 72-hour continuous operation at 37 °C.
Does the unit support validation documentation (IQ/OQ/PQ)?
Standard delivery includes factory calibration certificates for temperature and speed. Full IQ/OQ protocols and PQ templates are available upon request for regulated environments.
What maintenance is required for long-term reliability?
Annual verification of temperature uniformity (per ASTM E2234) and speed accuracy (using NIST-traceable tachometer) is recommended. No lubrication or belt replacement is needed due to brushless motor architecture.
