Revvity Chemagic Prime Automated Nucleic Acid Extraction and Reaction Setup System
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer | Revvity, Inc. |
| Product Type | Imported Instrument |
| Model | Chemagic Prime Automated High-Throughput Nucleic Acid Extraction Workstation |
| Technology | Magnetic Bead-Based Purification |
| Throughput | 2 × 96-well plates per run |
| Sample Tube Compatibility | Variable-format primary tubes (e.g., 13 mm round-bottom, 16 mm conical, 5–50 mL tubes) |
| Automation Capabilities | Integrated barcode scanning, reagent dispensing, eluate transfer, normalization, and PCR master mix assembly |
| Compliance | Designed for GLP/GMP environments with audit trail-ready operation |
Overview
The Revvity Chemagic Prime Automated Nucleic Acid Extraction and Reaction Setup System is an integrated, walk-away solution engineered for high-throughput molecular diagnostics, clinical research, and biobanking laboratories. It combines the precision liquid handling architecture of the JANUS® automated workstation with the proven magnetic bead-based nucleic acid isolation platform of the Chemagic™ 360 instrument. Unlike standalone extractors, the Chemagic Prime operates on a unified control framework to execute end-to-end workflows—from raw sample tube loading through magnetic bead binding, wash cycles, and elution, to downstream reaction setup—including qPCR, RT-qPCR, and NGS library preparation. Its core principle relies on paramagnetic particle (PMP) chemistry under programmable magnetic field gradients, ensuring consistent binding kinetics, efficient removal of inhibitors (e.g., heme, heparin, humic acids), and recovery of DNA/RNA across diverse biological matrices (whole blood, saliva, tissue homogenates, FFPE lysates, and viral transport media). The system is designed for reproducible performance in regulated environments where traceability, process standardization, and minimal manual intervention are critical.
Key Features
- Fully integrated hardware architecture: Seamless coordination between JANUS liquid handler and Chemagic 360 purification module eliminates inter-system handoffs and reduces cross-contamination risk.
- Variable-format sample tube loading: Accepts primary collection tubes without pre-aliquoting—supports 13 mm round-bottom, 16 mm conical, and 5–50 mL tubes via configurable rack adapters.
- Automated sample identification: Integrated high-resolution barcode scanner reads 1D/2D barcodes on sample tubes and reagent containers, enforcing sample integrity and enabling full chain-of-custody tracking.
- End-to-end reaction setup: Performs precise dispensing of master mixes, primers, probes, and templates into 96- or 384-well PCR plates with volume accuracy ≤±2% CV at 5 µL and ≤±1% CV at ≥20 µL (per ISO 8655-6).
- Dual-plate parallel processing: Simultaneously processes two 96-well plates—enabling up to 192 samples per run with fully independent protocol scheduling.
- On-board reagent management: Monitors reagent levels, tracks lot numbers, and enforces expiration date checks to support FDA 21 CFR Part 11 compliance when paired with validated software configurations.
Sample Compatibility & Compliance
The Chemagic Prime accommodates a broad spectrum of input materials, including anticoagulated whole blood (EDTA, citrate), buccal swabs, nasopharyngeal swabs in UTM/VTM, plasma, serum, urine, and cultured cells. Its magnetic bead chemistry has been validated per ISO 20387:2018 (biobanking) and meets analytical sensitivity requirements outlined in CLSI EP17-A2 for low-input samples (≥100 µL whole blood, ≥500 µL saliva). All purification protocols are aligned with manufacturer-recommended lysis buffers and wash formulations compatible with downstream applications such as Sanger sequencing, Illumina® library prep, and Thermo Fisher TaqMan® assays. The system supports audit trail generation, electronic signatures, and user-access controls required for GLP and GMP operations, and may be qualified for use in laboratories adhering to CAP, CLIA, and ISO/IEC 17025 standards.
Software & Data Management
Operation is managed through Revvity’s Chemagic Software Suite v4.x, a Windows-based application compliant with IEC 62304 Class B medical device software requirements. The interface provides drag-and-drop protocol building, real-time run monitoring with event logging (including temperature, pressure, and magnetic field status), and export of structured data in CSV, XML, and LIMS-compatible ASTM E1384 formats. All user actions, parameter changes, and error events are timestamped and stored with immutable audit trails. Optional integration with Laboratory Information Management Systems (LIMS) is supported via HL7 and RESTful API endpoints, enabling bidirectional sample registration, result push, and instrument status polling. Electronic signature functionality conforms to FDA 21 CFR Part 11 when deployed with appropriate infrastructure controls (e.g., password policies, role-based access, and digital certificate validation).
Applications
- Clinical microbiology labs performing high-volume respiratory virus panels (SARS-CoV-2, influenza A/B, RSV) and antimicrobial resistance screening.
- Pharmaceutical QC/QA units conducting identity testing of cell line banks using STR profiling and mycoplasma detection.
- Translational research cores preparing cfDNA libraries for liquid biopsy studies with input volumes as low as 1 mL plasma.
- National public health reference laboratories executing WHO-recommended genomic surveillance protocols requiring >1,000 extractions/week with full metadata traceability.
- IVD assay developers validating extraction efficiency across matrix types per ISO 13485 Annex C and ICH Q5A(R2) guidelines.
FAQ
Does the Chemagic Prime require dedicated exhaust or HVAC modifications?
No—its enclosed processing environment and sealed reagent modules eliminate need for external ventilation; standard laboratory ambient conditions (15–30°C, 30–70% RH) are sufficient.
Can legacy Chemagic 360 protocols be migrated to the Chemagic Prime?
Yes—protocol files are backward-compatible; however, liquid handling steps must be reconfigured in the JANUS module using the Chemagic Software Suite’s integrated method editor.
Is remote monitoring supported?
Yes—via Revvity Connect, a secure cloud-enabled dashboard providing real-time instrument status, consumables inventory alerts, and predictive maintenance notifications based on operational telemetry.
What regulatory documentation is provided with the system?
Each shipment includes a Factory Acceptance Test (FAT) report, Installation Qualification (IQ) and Operational Qualification (OQ) templates, and a Device Master Record (DMR)-aligned technical file per ISO 13485:2016.
How is calibration verified during routine use?
Calibration is performed using gravimetric verification kits traceable to NIST standards; automated calibration checks are embedded in daily startup routines and logged within the audit trail.
