Revvity JANUS G3 NGS Express (PLUS) Automated Liquid Handling Workstation

Overview

The Revvity JANUS G3 NGS Express (PLUS) is a modular, high-precision automated liquid handling workstation engineered specifically for end-to-end NGS library preparation workflows. Built upon the proven JANUS G3 platform architecture, it employs air displacement pipetting technology with multi-channel (up to 8-channel) and single-channel pipetting heads, enabling accurate volumetric dispensing across dynamic ranges from 0.5 µL to 1,000 µL. Its core design integrates tightly with Illumina, PacBio, Oxford Nanopore, and MGI-compatible library prep chemistries—including fragmentation, end repair, A-tailing, adapter ligation, size selection (via SPRI bead-based or dual-SPRI protocols), and PCR amplification. The system operates under a closed-deck, low-evaporation environment with temperature-controlled deck modules (4–45 °C), ensuring reproducible enzymatic reaction kinetics and minimizing sample loss during multi-step nucleic acid processing.

Key Features

• Modular deck configuration supporting up to 32 deck positions—compatible with standard SBS-format microplates (96-, 384-, and 1536-well), tube racks, magnetic stands, thermocyclers, centrifuges, and barcode readers.
• Integrated liquid level sensing, tip collision detection, and real-time aspiration/dispense monitoring for enhanced assay robustness and failure mitigation.
• Dual pipetting head option: simultaneous use of fixed-volume and variable-volume pipetting channels to optimize throughput and flexibility in parallel workflows.
• Pre-validated, vendor-verified NGS application packages—including TruSight Oncology, Nextera XT, NEBNext Ultra II, and KAPA HyperPlus—delivered with method files, calibration protocols, and QC checklists.
• Robust mechanical architecture with ISO Class 5 (Class 100) cleanroom-rated components; compliant with IEC 61000-6-2/6-3 for electromagnetic compatibility and UL/CSA safety standards.

Sample Compatibility & Compliance

The JANUS G3 NGS Express (PLUS) accommodates diverse nucleic acid inputs—including genomic DNA (gDNA), FFPE-derived DNA, cfDNA, total RNA, and cDNA—with input volumes ranging from 1 ng to 1 µg per reaction. It supports both manual and automated SPRI bead cleanup, capillary electrophoresis integration (e.g., Agilent TapeStation or Bioanalyzer via optional robotic arm interface), and post-library quantification via qPCR or fluorometric assays. All preloaded methods are developed and verified under GLP-aligned documentation practices. The system supports 21 CFR Part 11-compliant electronic records and signatures when deployed with Interchange™ software and validated user access controls, making it suitable for regulated environments engaged in clinical assay development, LDT implementation, or companion diagnostic workflow validation.

Software & Data Management

MethodBuilder™ provides a graphical, drag-and-drop protocol authoring environment with embedded error-checking logic, pipette tip tracking, and cross-platform method portability. Interchange™ serves as the central data orchestration layer—enabling bidirectional communication with LIMS (e.g., LabVantage, STARLIMS), ELN systems (e.g., Benchling, LabArchives), and instrument-specific analytics platforms. Audit trails capture all user actions, method modifications, and run execution metadata with immutable timestamps. Raw log files are exportable in CSV and XML formats for external QA review or regulatory submission (e.g., IDE, PMA, or IVDR technical documentation).

Applications

• Automated whole-genome sequencing (WGS) and exome library construction for population-scale genomics studies.
• Targeted panel library prep for oncology biomarker discovery and minimal residual disease (MRD) monitoring.
• Single-cell RNA-seq and ATAC-seq library generation with integrated cell lysis and barcoding steps.
• High-throughput CRISPR screening library production with pooled guide RNA synthesis and lentiviral packaging support.
• Regulatory-grade NGS assay deployment in CAP-accredited or CLIA-certified laboratories performing somatic variant calling or germline risk assessment.

FAQ

Is the JANUS G3 NGS Express (PLUS) compatible with third-party reagent kits?
Yes—Revvity provides open API access and method templates for integration with non-proprietary library prep chemistries, subject to customer validation under internal SOPs.
What level of technical support is included post-installation?
All systems ship with a 12-month Application Onboarding Package, including on-site installation qualification (IQ), operational qualification (OQ), remote method optimization sessions, and access to Revvity’s global Field Applications Scientist network.
Can the system be upgraded to support new NGS chemistries after purchase?
Yes—hardware modularity allows field-installable upgrades including additional pipetting heads, thermal modules, and imaging peripherals; software updates are delivered quarterly via secure Revvity Connect portal.
Does the platform support walk-away operation for overnight library prep runs?
Yes—fully unattended operation is enabled via integrated environmental monitoring, automatic tip disposal, and conditional branching logic that halts execution upon detected anomalies (e.g., insufficient reagent volume or plate misalignment).