Revvity VICTOR Nivo Multimode Microplate Reader
| Brand | Revvity |
|---|---|
| Origin | Finland |
| Manufacturer | Revvity (Oy, Turku) |
| Product Type | Multimode Microplate Reader |
| Automation Level | Fully Automated |
| Assay Modes | Endpoint, Kinetic, Scanning |
| Curve Fitting Options | Linear, Log-Log, 4PL, 5PL, Quadratic, Sigmoidal |
| Detector Types | Silicon Photodiode & High-Sensitivity PMT |
| Wavelength Range | 230–1000 nm |
| Absorbance Range | 0–4 OD |
| Absorbance Linearity | ±0.01 OD (0–2 OD) |
| Wavelength Switching Time | ≤10 s |
| Absorbance Precision | <0.1% CV |
Overview
The Revvity VICTOR Nivo Multimode Microplate Reader is an engineered platform for quantitative detection across six core optical modalities: absorbance (ABS), luminescence (LUM), fluorescence intensity (FI), time-resolved fluorescence (TRF), fluorescence polarization (FP), and Alpha technology (Amplified Luminescent Proximity Homogeneous Assay). Designed on the foundation of Revvity’s established VICTOR X architecture, the Nivo integrates full multimodal capability into a compact, benchtop footprint measuring just 32 × 45 × 30 cm — the smallest in its class. Its modular optical path employs dual detection systems: a high-stability silicon photodiode optimized for absorbance measurements from 230 to 1000 nm, and a low-noise, thermoelectrically cooled photomultiplier tube (PMT) for ultra-sensitive luminescent and fluorescent assays. The system supports both top- and bottom-reading configurations (excluding ABS and Alpha modes), enabling flexible assay design for adherent cells, suspension cultures, and bead-based immunoassays.
Key Features
- Compact benchtop form factor compatible with biosafety cabinets, incubators, and shared lab workspaces
- Dual-detector architecture: photodiode for absorbance (0–4 OD, linearity maintained up to 2 OD) and PMT for low-background luminescence/fluorescence
- Integrated 32-position filter wheel with motorized positioning and automatic calibration verification
- Onboard temperature control (ambient to 45 °C, ±0.5 °C stability) and optional CO2/O2 gas regulation module for live-cell kinetic assays
- Optional integrated reagent dispensers (up to two channels) supporting precise, low-volume (<5 µL) additions during kinetic runs
- Wi-Fi-enabled remote operation via web browser or dedicated tablet application — no local installation required
- Workflow-driven software interface with role-based access control and audit-trail logging
Sample Compatibility & Compliance
The VICTOR Nivo accommodates standard microplate formats including 6-, 12-, 24-, 48-, 96-, 384-, and 1536-well plates, as well as specialized plates (e.g., black/clear-bottom, white opaque, UV-transparent). It supports diverse sample types: purified proteins, cell lysates, whole cells (adherent and suspension), primary isolates, and antibody-conjugated beads. All optical measurements adhere to ISO 15197:2013 principles for photometric precision and traceability. For regulated environments, the Enhanced Security software package provides electronic signatures, user authentication, change control logs, and full 21 CFR Part 11 compliance — validated for GxP workflows including GLP and GMP. Instrument qualification documentation (IQ/OQ/PQ protocols) and risk assessments are available upon request.
Software & Data Management
VICTOR Nivo Software is built on a secure, client-server architecture with local or network-deployed database options. It features intuitive workflow templates for common assays (e.g., ELISA, cell viability, cAMP, kinase activity), customizable protocol scripting (JavaScript-based), and real-time data visualization with overlay kinetics, plate heatmaps, and statistical outlier detection. Raw data export is supported in CSV, XML, and SDMX-compliant formats; integration with LIMS and ELN platforms is achieved via RESTful API and ASTM E1384-compliant messaging. Audit trails record all user actions, parameter changes, and instrument events with immutable timestamps — fully compliant with ALCOA+ data integrity standards.
Applications
- Quantitative ELISA and immunoassay development (absorbance, TRF, AlphaLISA)
- Cell-based functional assays: Ca2+ flux (FLIPR-compatible dyes), apoptosis (caspase-3/7 luminescence), mitochondrial membrane potential (JC-1, TMRM)
- High-throughput screening (HTS) of small-molecule libraries using FP-based binding assays or AlphaScreen proximity assays
- Kinetic enzyme characterization (e.g., phosphatase, protease, dehydrogenase activity) with sub-second interval sampling
- Microbial growth monitoring and antimicrobial susceptibility testing (OD600 kinetics)
- Reporter gene assays (luciferase, GFP variants) under controlled CO2/humidity conditions
FAQ
Does the VICTOR Nivo support FDA 21 CFR Part 11 compliance?
Yes — when configured with Enhanced Security software, it provides electronic signatures, audit trails, role-based permissions, and system validation support for regulated laboratories.
Can I perform both top- and bottom-reading in the same assay run?
No — reading orientation must be selected per protocol; top-read is required for luminescence and Alpha, while bottom-read is recommended for adherent cell assays in FI/TRF/FP modes.
What is the minimum detectable luminescence signal?
The PMT achieves <10 amol ATP (using ENLITEN kit) at 1-second integration, with typical background <5 RLUs in white 96-well plates.
Is service and calibration support available globally?
Yes — Revvity operates certified service centers across North America, EMEA, and APAC, offering on-site calibration, preventive maintenance, and IQ/OQ execution per ISO/IEC 17025 standards.
Can the instrument be integrated into automated liquid handling workflows?
Yes — it supports standard deck interfaces (SLAS/ANSI footprint) and communicates via RS-232, USB, or Ethernet with third-party robotics via defined command protocols.
