Shanghai Youyi LHS-250CL Low-Temperature Temperature and Humidity Controlled Chamber

Overview

The Shanghai Youyi LHS-250CL is a vertically oriented, air-cooled temperature and humidity controlled chamber engineered for precise, long-term environmental simulation across demanding scientific and industrial applications. It operates on the principle of active thermoelectric and vapor-compression refrigeration combined with resistive steam humidification and PID-regulated air recirculation—enabling stable, reproducible control of both thermal and hygrometric parameters within a single enclosed workspace. Designed for laboratories requiring stringent environmental conditioning—including biobanking, pharmaceutical stability testing, electronics reliability validation, and materials aging studies—the LHS-250CL delivers certified performance in compliance with foundational requirements of ISO 17025-accredited calibration practices and supports alignment with ICH Q1–Q5 guidelines for environmental stress testing of biological and chemical products.

Key Features

• Microprocessor-based PID controller with 5.7-inch backlit LCD interface, supporting up to 99 programmable cycles, each comprising 30 customizable segments (max. 99 h 99 min per segment) for complex multi-step environmental profiles.
• Double-glazed observation door with integrated silicone gasket and vacuum-sealed glass panel—minimizing thermal bridging while enabling non-intrusive visual monitoring without compromising chamber integrity.
• High-efficiency air-circulation system utilizing optimized duct geometry and tangential blower assembly to ensure uniform temperature distribution (±0.5 °C) and humidity homogeneity (±3 %RH) throughout the 250 L working volume.
• Corrosion-resistant mirror-finish stainless steel interior with radius-rounded corners and adjustable shelf supports—facilitating GMP-compliant cleaning, validation, and residue-free maintenance.
• Eco-conscious refrigeration using R134a and R406a blended refrigerant; low-global-warming-potential (GWP) formulation compliant with EU F-Gas Regulation (EU No 517/2014) and aligned with current OEM sustainability mandates.
• Integrated water reservoir with submerged stainless steel heating element and high-accuracy capacitive humidity sensor (calibration-traceable to NIST standards) for responsive, low-drift RH control across the full 30–95 %RH range.
• Modular mobility system featuring dual-mode casters—360° swivel with locking brake plus fixed leveling feet—for secure positioning on laboratory-grade anti-vibration flooring or ISO Class 5–8 cleanroom subfloors.

Sample Compatibility & Compliance

The LHS-250CL accommodates diverse sample formats including cryovials, serum bottles, petri dishes, PCB assemblies, polymer films, and packaged medical devices—without risk of condensation-induced degradation or thermal shock. Its validated operating envelope satisfies critical preconditions for accelerated stability protocols under ICH Q1A(R3), USP , and ASTM E145–22 (Standard Specification for Gravity-Convection and Forced-Ventilation Ovens). While not inherently 21 CFR Part 11–compliant, optional RS485/USB interfaces enable integration with validated LIMS or ELN platforms supporting electronic audit trails, user access controls, and data integrity workflows required under GLP and GMP environments.

Software & Data Management

When equipped with the optional USB/RS485 communication module and proprietary data acquisition software, the LHS-250CL supports real-time parameter logging at user-defined intervals (1 s to 60 min), automatic CSV export, trend visualization, and alarm-triggered event tagging. Raw data files include timestamped metadata (controller firmware version, sensor serial numbers, calibration dates), fulfilling ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). The software architecture permits configuration of custom alert thresholds for temperature deviation, humidity drift, door-open duration, and power interruption—each generating time-stamped notifications via email or local sounder.

Applications

• Bio-banking: Long-term storage of plasma, PBMCs, tissue sections, and viral vectors at −40 °C under controlled humidity to prevent ice recrystallization and desiccation.
• Pharmaceutical development: ICH-aligned accelerated stability testing (e.g., 40 °C/75 %RH, 30 °C/65 %RH) for drug substances and primary packaging compatibility assessment.
• Electronics reliability: Thermal cycling and damp heat exposure per JEDEC JESD22-A104E and MIL-STD-883H Method 1030.8 for solder joint fatigue analysis.
• Polymer science: Hygrothermal aging of composites, adhesives, and elastomers to quantify moisture diffusion coefficients and Tg shifts.
• Medical device validation: ISO 11607–2 compliant accelerated aging of sterile barrier systems under elevated temperature/humidity conditions.

FAQ

What is the minimum achievable temperature with full humidity control active?
The chamber maintains humidity down to 30 %RH across the entire −40 °C to +80 °C range; however, sustained operation below −20 °C at >70 %RH is not recommended due to condensation risk on internal surfaces.
Is the unit suitable for continuous unattended operation?
Yes—equipped with independent over-temperature cut-off, compressor high-pressure protection, and auto-restart logic after power recovery; optional external alarm relay output enables integration into facility-wide monitoring systems.
Can the chamber be validated per IQ/OQ protocols?
Yes—manufacturer-supplied as-built documentation includes factory calibration certificates for temperature and humidity sensors, airflow mapping reports, and electrical safety test records—serving as baseline evidence for user-executed qualification.
Does the unit support external data logging without optional hardware?
No—real-time digital data export requires the RS485/USB interface module; analog voltage outputs (0–5 V DC) for temperature and humidity are not provided as standard.
What is the maximum load capacity for shelf loading?
The chamber supports up to 19 kg per shelf (maximum 3 shelves installed); total payload must not exceed 57 kg to preserve thermal uniformity and avoid airflow obstruction.