Shimadzu SIL-10ADvp Used Autosampler

Overview

The Shimadzu SIL-10ADvp is a high-precision, modular autosampler engineered for integration with Shimadzu LC systems—including the LC-10AD, LC-2010, and LC-2030 series—supporting robust, reproducible sample introduction in regulated and research-grade liquid chromatography workflows. As a second-generation design within Shimadzu’s SIL-10A platform, the SIL-10ADvp employs a dual-syringe, positive-displacement injection mechanism with quantitative loop sampling, ensuring minimal carryover and high volumetric accuracy across variable injection volumes. Its architecture follows ISO/IEC 17025-aligned design principles for analytical instrumentation, emphasizing mechanical stability, thermal management, and positional repeatability. Unlike fixed-loop injectors, the SIL-10ADvp supports both fixed- and variable-volume injection modes via software-configurable syringe draw-and-deliver sequences, enabling method flexibility without hardware reconfiguration.

Key Features

• Dual-syringe, positive-displacement metering system for enhanced accuracy and reduced pulsation-induced variability
• Programmable needle wash cycle—configurable before and after each injection to minimize cross-contamination
• Modular cooling support via optional Sample Cooler S (compact) or Sample Cooler L (high-capacity), maintaining sample integrity at 4–70 °C with ±0.5 °C stability
• Multi-format sample carrier compatibility: 100-position 1.5 mL vial tray (at 60 °C cooling), 80-position 4 mL vial tray (at 50 °C cooling), and dual 96-well microtiter plate (MTP) support for high-throughput screening
• Injection precision validated per ASTM E2639-20: ≤0.5% RSD at 10 µL under standard operating conditions; ≤1.0% RSD across full 1–5000 µL range with appropriate optional modules
• Chemical resistance certified for aqueous and organic mobile phases across pH 1–10, compliant with USP chromatographic system suitability requirements

Sample Compatibility & Compliance

The SIL-10ADvp accommodates standard 1.5 mL and 4 mL screw-thread or crimp-top vials, as well as ANSI/SLAS-compliant 96-well plates, supporting both routine QC and method development applications. All wetted parts—including the injection needle, rotor seal, and quantitative loop—are constructed from chemically inert materials (e.g., PEEK, sapphire, and stainless steel 316L) to ensure compatibility with aggressive solvents (e.g., THF, DMSO, concentrated acids/bases) and prevent leaching or adsorption artifacts. The unit meets electromagnetic compatibility (EMC) requirements per IEC 61326-1 and safety standards per IEC 61010-1. When deployed in GLP/GMP environments, its operational logs—accessible via Shimadzu LabSolutions software—support audit trail generation aligned with FDA 21 CFR Part 11 requirements when paired with appropriate system-level access controls and electronic signature configuration.

Software & Data Management

Fully integrated with Shimadzu LabSolutions LC/GC software (versions 5.9x and later), the SIL-10ADvp supports method-driven sequence programming, real-time status monitoring, and event-triggered diagnostics. Injection parameters—including volume, wash solvent selection, dwell time, and tray position mapping—are stored within instrument methods and exported in .csv or .xml format for traceability. System logs record every injection event (timestamp, volume, vial ID, error flags), enabling retrospective analysis during investigations or regulatory inspections. Remote diagnostics via Ethernet interface allow for predictive maintenance alerts based on actuator cycle counts and pressure deviation trends—critical for minimizing unplanned downtime in multi-shift laboratory operations.

Applications

This refurbished SIL-10ADvp is routinely deployed in pharmaceutical stability studies (ICH Q5C), environmental residue analysis (EPA Method 8330B), food safety testing (AOAC 2012.01), and clinical bioanalysis (FDA Bioanalytical Method Validation Guidance). Its low carryover (<0.01%) and thermal stabilization make it suitable for thermolabile analytes such as peptides, oligonucleotides, and labile metabolites. In method transfer scenarios between R&D and QC labs, the SIL-10ADvp’s consistent injection profile ensures retention time and peak area reproducibility across instruments—reducing validation burden and accelerating regulatory submissions.

FAQ

Is this unit fully tested and calibrated prior to shipment?
Yes—each refurbished SIL-10ADvp undergoes functional verification including syringe linearity check, injection precision assessment (10 µL × 6 replicates), needle wash efficiency test, and cooling module temperature validation across the full 4–70 °C range.
Does it include original Shimadzu documentation and firmware version history?
Yes—shipping package includes original service manual, calibration certificate (traceable to NIST standards), and firmware revision log confirming compliance with LabSolutions v5.95 or later.
Can it be integrated with non-Shimadzu HPLC systems?
While designed for native Shimadzu LC control, third-party integration is possible via RS-232 or TTL trigger signals for basic start/stop commands; however, full parameter control and diagnostic feedback require Shimadzu LC driver modules.
What consumables are recommended for long-term reliability?
Shimadzu genuine rotor seals (Part No. 228-32541-91), PEEK injection needles (228-32542-91), and quantitative loops (228-32543-91, 100 µL default) are strongly advised to maintain specified accuracy and avoid warranty voidance.
Is extended warranty or service contract available?
Yes—optional 12- or 24-month comprehensive service agreements are available, covering labor, parts, preventive maintenance, and remote technical support by Shimadzu-certified field engineers.