SOPOTOP 723PC Automatic Wavelength UV-Vis Spectrophotometer

Overview

The SOPOTOP 723PC is a single-beam ultraviolet-visible (UV-Vis) spectrophotometer engineered for routine quantitative and qualitative analysis in academic laboratories, quality control environments, and industrial R&D settings. It operates on the principle of Beer-Lambert law-based absorbance measurement, utilizing a Czerny-Turner (C-T) monochromator equipped with a 1200-line/mm holographic grating to deliver precise wavelength selection across a broad spectral range of 325–1100 nm. With automatic wavelength scanning capability and a fixed spectral bandwidth of 2 nm, the instrument ensures high photometric reproducibility and spectral resolution suitable for applications ranging from concentration determination of colored solutions to nucleic acid and protein quantification. Its robust optical architecture, combined with temperature-stabilized electronics and low-stray-light design, supports reliable performance under standard laboratory conditions without requiring external calibration references for daily operation.

Key Features

• Automatic wavelength positioning with 0.1 nm step resolution and ±1.0 nm absolute accuracy, enabling rapid method setup and inter-sample wavelength repeatability.
• 4.3-inch high-resolution color TFT touchscreen interface (480 × 272 pixels) supporting intuitive gesture-based navigation, real-time spectrum preview, and on-device data visualization.
• Direct concentration readout functionality with customizable concentration factors and multi-point standard curve fitting (linear, quadratic, cubic, or piecewise).
• Integrated USB 2.0 port for direct data export to external storage devices; supports CSV and Excel-compatible file formats for seamless integration into LIMS or statistical analysis platforms.
• Comprehensive photometric measurement modes: fixed-wavelength absorbance/transmittance, full-spectrum scanning (A/T/E), kinetic time-course analysis, multi-wavelength quantitation, and 3D spectral mapping.
• Preconfigured DNA/protein analysis module compliant with standard A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀ ratio calculations per established biochemical protocols (e.g., NIH Guidelines, Thermo Fisher Qubit methodology).

Sample Compatibility & Compliance

The 723PC accommodates standard 10 mm pathlength cuvettes (glass, quartz, or plastic), with optional accessories available for micro-volume or flow-cell configurations. It meets general-purpose analytical requirements aligned with ISO 9001-certified manufacturing practices and conforms to electromagnetic compatibility (EMC) standards IEC 61326-1 for laboratory equipment. While not certified as GMP-compliant out-of-the-box, the optional UV-Solution software package includes an audit trail module compliant with FDA 21 CFR Part 11 when deployed on validated Windows OS platforms—supporting electronic signatures, user access controls, and immutable activity logs required for regulated QC/QA workflows.

Software & Data Management

UV-Solution is a Windows-based PC workstation software designed specifically for the 723PC platform. It provides full remote instrument control, real-time data acquisition, and post-processing capabilities including spectral smoothing (Savitzky-Golay), derivative spectroscopy (1st–3rd order), peak/valley detection, overlay comparison, and mathematical operations between spectra. All measurement results—including raw absorbance values, processed curves, and metadata (date/time, operator ID, method name)—are stored in structured binary project files (.usp) with embedded checksums. Export functions support .xlsx (Excel), .csv (tabular), .bmp (raster image), and .txt (ASCII) formats. The software architecture supports multi-user configuration and integrates with enterprise directory services via Windows Active Directory authentication.

Applications

This spectrophotometer serves core analytical needs across multiple domains: environmental water quality testing (e.g., nitrate, phosphate, COD assays); pharmaceutical excipient and active ingredient verification per USP ; food and beverage colorimetric analysis (e.g., anthocyanin content, caramel index); educational chemistry labs for kinetics experiments and equilibrium constant determination; and life science labs performing routine nucleic acid purity assessment and Bradford/Lowry protein assays. Its spectral fidelity and operational stability make it particularly suitable for method transfer validation where traceability and inter-instrument comparability are critical.

FAQ

Is the 723PC compliant with GLP or GMP requirements?
The base instrument complies with general laboratory equipment standards. Full 21 CFR Part 11 compliance requires deployment of the optional UV-Solution Audit Trail Edition on a validated Windows system with controlled user access and documented change management procedures.
What cuvette materials are supported?
Standard 10 mm square cuvettes made of optical glass (340–2500 nm), fused silica/quartz (190–2500 nm), or disposable polystyrene (350–800 nm) are compatible. Quartz cuvettes are recommended for measurements below 340 nm.
Can the instrument perform simultaneous multi-wavelength measurements?
No—it is a single-beam system and acquires data sequentially at discrete wavelengths. However, multi-wavelength quantitation mode allows rapid switching between up to three user-defined wavelengths within a single run.
Does the software support automated calibration verification?
UV-Solution includes built-in wavelength and photometric accuracy verification routines using NIST-traceable holmium oxide and neutral density filters, with pass/fail reporting against ISO 6507-1 and ASTM E275 guidelines.
Is remote instrument control possible over LAN or Ethernet?
The 723PC communicates exclusively via USB 2.0. Network connectivity requires an external USB-to-Ethernet server configured at the host PC level—not natively supported by the instrument firmware.