Thermo Fisher Herasafe™ KS II Class II Biological Safety Cabinet
| Brand | Thermo Fisher |
|---|---|
| Origin | Germany |
| Manufacturer | Thermo Fisher Scientific |
| Product Type | Imported |
| Model Numbers | 51022481, 51022482, 51022483, 51022484 |
| External Dimensions (L×W×H) | 1100–2000 mm |
| Internal Dimensions (L×W×H) | 900–1800 mm |
| HEPA Filter Efficiency at MPPS | ≥99.995% |
Overview
The Thermo Fisher Herasafe™ KS II Class II Biological Safety Cabinet is an engineered containment solution designed for safe handling of low- to moderate-risk biological agents (BSL-1 to BSL-2), including recombinant DNA constructs, human cell lines, and select pathogenic microorganisms. It operates on the principle of inward airflow (personnel protection), downward laminar airflow (product protection), and recirculated/ducted exhaust air (environmental protection) — fully compliant with the dual-protection paradigm defined in EN 12469 and NSF/ANSI 49. The cabinet employs a certified HEPA filter with ≥99.995% efficiency at the Most Penetrating Particle Size (MPPS), typically 0.1–0.2 µm, ensuring robust particulate retention across the full operational lifecycle. Its aerodynamic architecture integrates a rear plenum, perforated work surface, and precisely calibrated airfoil geometry to maintain stable, uniform inflow and downflow velocities — critical for maintaining ISO Class 5 (Class 100) conditions within the work area.
Key Features
- SmartFlow™ digital airflow control system continuously monitors and dynamically adjusts motor speed to sustain nominal inflow (≥0.5 m/s) and downflow (≥0.3 m/s) velocities despite filter loading or transient obstructions — eliminating manual recalibration.
- Integrated pressure sensors distributed across the front grille and rear plenum provide real-time verification of uniform airflow distribution over the entire work surface.
- Digital Performance & Status Indicator (PER display) mounted at eye level on the rear wall delivers immediate visual feedback: “Safe”, “Safe – Maintenance Due”, or “Not Operational”, based on validated airflow thresholds and system diagnostics.
- Electrically actuated sash with adjustable height stop (25 cm standard opening, 10° forward tilt) enables ergonomic access while minimizing turbulence; sash movement is synchronized with airflow compensation via SmartFlow™.
- Brushless DC motor technology reduces power consumption by up to 40% versus traditional AC motors and lowers thermal load inside the lab environment.
- Frameless tempered glass sash and side windows eliminate visual obstructions; seamless stainless steel work surface features integrated liquid-retaining channels and removable grid panels for easy decontamination.
- Aerosol-tight sash seal design maintains integrity during standby mode, preserving containment even when unattended.
Sample Compatibility & Compliance
The Herasafe™ KS II accommodates standard laboratory workflows involving pipetting, centrifugation (with appropriate rotor containment), cell culture, and microbiological assays. Its internal volume supports benchtop instruments up to 1800 mm depth and accommodates modular work surfaces (monolithic or segmented configurations). All units undergo independent certification by TÜV Nord per EN 12469:2021 for 230 V models and NSF/ANSI 49-2022 for 120 V variants. Certification includes rigorous testing of airflow velocity profiles, smoke pattern visualization, particle challenge (via polydisperse NaCl aerosol), and microbial containment (using Bacillus atrophaeus spores). Units are compatible with GLP and GMP environments where audit-ready documentation, traceable calibration records, and periodic performance verification (e.g., annual recertification per ISO 14644-3) are required.
Software & Data Management
While the Herasafe™ KS II operates as a standalone hardware platform without cloud connectivity, its embedded firmware supports full audit trail functionality per FDA 21 CFR Part 11 requirements when integrated into validated laboratory information management systems (LIMS). The PER display logs timestamped events including UV lamp runtime, total operational hours, alarm triggers (e.g., airflow deviation >±10%), and maintenance alerts. Optional RS-485 or Modbus RTU interfaces enable integration with building management systems (BMS) for centralized monitoring of cabinet status, energy usage, and alarm escalation. All firmware updates are delivered via secure USB-based protocol with cryptographic signature validation.
Applications
- Primary and secondary cell culture under aseptic conditions
- Handling of genetically modified organisms (GMOs) requiring BSL-2 containment
- Preparation of diagnostic reagents and clinical specimens
- Pharmaceutical QC testing involving viable biologics
- Educational laboratories conducting microbiology and molecular biology instruction
- Research involving viral vectors, lentiviruses, and other replication-deficient agents
FAQ
What standards does the Herasafe™ KS II comply with?
It is independently certified to EN 12469:2021 (for 230 V models) and NSF/ANSI 49-2022 (for 120 V models), covering airflow performance, containment integrity, and structural safety.
Is the HEPA filter certified at MPPS?
Yes — all factory-installed HEPA filters are individually tested and certified to retain ≥99.995% of particles at the Most Penetrating Particle Size (MPPS), verified per EN 1822-3.
Can the cabinet be integrated into a facility’s BMS?
Yes — optional serial communication modules support Modbus RTU or RS-485 protocols for remote status monitoring and alarm forwarding.
What maintenance intervals are recommended?
HEPA filter replacement is advised every 3–5 years depending on usage intensity; annual third-party recertification per ISO 14644-3 is required for regulated environments.
Does the unit support UV decontamination validation?
UV lamp runtime is tracked and displayed in hours; however, UV intensity verification requires external radiometric measurement per IEC 62471, not performed internally.
