TLYON TLYON-FJ-30L Stainless Steel Fermenter
| Brand | TLYON |
|---|---|
| Origin | Sichuan, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | TLYON-FJ-30L |
| Price Range | USD 14,000–28,000 |
| Vessel Volume | 30 L |
| Aeration System | Quad-channel gas mixing and sterile air delivery system |
| pH Control | 0–14 pH (display), ±0.01 pH resolution, auto-setpoint control |
| Dissolved Oxygen (DO) Control | 0–200% saturation (display), 0.1% resolution |
| Temperature Control | 0–150 °C (measurement), ±0.2 °C accuracy, 0.1 °C resolution |
| Working Pressure | 0.3 MPa |
| Vessel Material | ASTM A240 316 stainless steel |
| Sterile Air Filtration | Triple-stage membrane filtration (5 µm pre-filter, 0.1 µm primary, 0.01 µm final |
| Feed System | Peristaltic pump with constant-flow precision |
| Antifoam Control | Conductivity-based automatic dosing module (10–100,000 Ω range) |
| Biogas Measurement | BMP gas volume detection (0–5000 mL/h, ±0.01 mL error, 6.5 mL resolution) and real-time gas flow rate monitoring (0–10 L/min, ±0.1% FS accuracy, 0.01 mL/min resolution) |
| Control Architecture | MC-BIO8000 embedded PLC-based lower-level controller + PC-based upper-level HMI with audit trail and data logging |
Overview
The TLYON TLYON-FJ-30L Stainless Steel Fermenter is a fully integrated, laboratory-scale bioreactor engineered for rigorous microbial fermentation process development under controlled, reproducible, and GxP-aligned conditions. Designed around the Couette-type stirred-tank configuration, it employs direct-drive impeller agitation, jacketed temperature regulation, and multi-parameter online sensing to support aerobic, microaerobic, and anaerobic cultivations—including yeast, bacteria, filamentous fungi, and recombinant cell lines. Its pressure-rated 30 L vessel (ASME Section VIII Div. 1 compliant design basis), constructed from ASTM A240 316 stainless steel with electropolished interior (Ra ≤ 0.4 µm), ensures long-term corrosion resistance, cleanability, and compliance with ISO 13485 and cGMP facility prerequisites. The system integrates sterile air supply via triple-stage membrane filtration (0.01 µm final grade), steam-in-place (SIP) capability up to 121 °C, and full validation-ready instrumentation—making it suitable for strain characterization, media optimization, scale-down modeling, and regulatory batch documentation in R&D and pilot manufacturing environments.
Key Features
- 30 L working volume bioreactor with 0.3 MPa maximum operating pressure and integrated SIP/sterilization manifold
- Quad-channel mass flow-controlled gas blending system (O₂, N₂, CO₂, air) for precise dissolved oxygen (DO) setpoint modulation and pH-driven gassing strategies
- High-fidelity sensor suite: platinum RTD (Pt100) for temperature (±0.2 °C), ISFET-based pH probe (0–14 pH, ±0.01 pH resolution), polarographic DO electrode (0–200% saturation, 0.1% resolution), and differential pressure transducer (0–0.4 MPa)
- Automated peristaltic feed system with programmable volumetric dosing profiles for acid/base, antifoam, and nutrient supplementation
- Dual-mode biogas quantification: volumetric BMP measurement (0–5000 mL/h, ±0.01 mL absolute error) and high-resolution transient flow monitoring (0–10 L/min, ±0.1% full-scale accuracy)
- MC-BIO8000 embedded PLC controller with deterministic scan cycle (<50 ms), real-time PID tuning, and local data buffering (≥72 h at 1 Hz sampling)
Sample Compatibility & Compliance
The TLYON-FJ-30L accommodates diverse biological systems including E. coli, Saccharomyces cerevisiae, Pichia pastoris, Aspergillus niger, and mixed-culture consortia used in bioethanol, organic acid, enzyme, and biopolymer production. Its design supports cultivation in complex, particulate-rich substrates—such as corn mash, molasses-based broths, and lignocellulosic hydrolysates—without compromising sterility or sensor stability. All wetted components meet USP Class VI and FDA 21 CFR Part 11 requirements for electronic records and signatures when deployed with validated software configurations. The system conforms to ISO 20957-1 (bioreactor safety), ASTM E2500-13 (user requirement specification guidance), and supports IQ/OQ/PQ protocol execution. Documentation packages include FAT/SAT reports, material traceability certificates (MTRs), and 3.1 mill certificates for all 316 SS fabrication.
Software & Data Management
The fermenter operates with a dual-tier architecture: the MC-BIO8000 PLC handles real-time closed-loop control (temperature, pH, DO, agitation, gas flow, pressure), while the upper-level PC-based HMI provides visualization, historical trending, alarm management, and exportable CSV/Excel data archives. Software features include configurable audit trails (user login, parameter changes, manual overrides), electronic signature workflows, and time-stamped event logging aligned with ALCOA+ principles. Data export complies with ASTM E2500 Annex D and supports integration into LIMS or MES platforms via OPC UA (v1.04) or Modbus TCP. All firmware and HMI versions are version-controlled and subject to change control procedures per ISO 13485 clause 7.5.4.
Applications
- Strain performance screening under varied pH, DO, and feeding regimes
- Media formulation studies—including carbon/nitrogen source ratio optimization and trace element titration
- Fermentation kinetic modeling (µmax, YX/S, qO2, qCO2) using synchronized online gas analysis
- Process robustness testing across inoculum density, temperature ramping, and shear stress gradients
- Biohydrogen, biomethane, and volatile fatty acid production assays requiring precise biogas volumetry
- Technology transfer from lab to pilot scale via scalable operating envelopes (kLa, P/V, tip speed, mixing time)
FAQ
Is the TLYON-FJ-30L compliant with FDA 21 CFR Part 11 for electronic records?
Yes—when configured with password-protected user roles, electronic signatures, and immutable audit trails enabled in the HMI software, the system meets predicate rule requirements for record integrity and accountability.
Can the system perform sterilization-in-place (SIP)?
Yes—the vessel, sparger, and internal sensors are rated for 121 °C saturated steam exposure; SIP protocols are preloaded and configurable within the MC-BIO8000 controller.
What level of validation support is provided?
TLYON supplies URS templates, FAT/SAT checklists, calibration certificates for all primary sensors, and material compliance documentation—enabling customers to execute IQ/OQ/PQ under internal or third-party QA oversight.
Does the system support remote monitoring and control?
Remote access is supported via secure VPN tunneling to the HMI PC; however, real-time control commands require authenticated session initiation and are logged with operator ID and timestamp.
Are spare parts and sensor replacements available globally?
All critical consumables—including 0.01 µm sterile air filters, ISFET pH probes, and polarographic DO electrodes—are stocked by authorized TLYON distribution partners in North America, EU, and APAC regions, with lead times under 10 business days.
