Wufeng EX1800 UHPLC/HPLC Two-Dimensional Liquid Chromatography System
| Brand | Wufeng |
|---|---|
| Origin | Shanghai, China |
| Model | EX1800 UHPLC/HPLC |
| Instrument Type | Two-Dimensional Ultra-High-Performance Liquid Chromatograph |
| Flow Rate Range | 0.001–10 mL/min |
| Maximum Operating Pressure | 120 MPa (UHPLC mode) / 60–70 MPa (HPLC mode) |
| Flow Precision | ≤0.06% RSD |
| Autosampler Capacity | 330 positions (1 mL vials), 192 positions (2 mL vials), 108 positions (4 mL vials), 48 positions (10 mL vials) |
| Injection Volume Range | 0.1–120 µL |
| Column Oven Temperature Range | 10 °C below ambient to 80 °C |
| UV-Vis Detection Wavelength Range | 190–700 nm (standard) / optional 190–900 nm |
| Data Acquisition Rate | up to 100 Hz |
Overview
The Wufeng EX1800 is a dual-mode two-dimensional liquid chromatography system engineered for high-resolution separation of complex matrices in research, quality control, and regulatory environments. It integrates both ultra-high-performance liquid chromatography (UHPLC) and high-performance liquid chromatography (HPLC) capabilities within a single platform—enabling method flexibility without hardware reconfiguration. The system operates on the principle of comprehensive two-dimensional liquid chromatography (LC×LC), where effluent from a first-dimension column is sequentially transferred to a second-dimension column via a high-speed, low-dead-volume switching valve. This architecture significantly enhances peak capacity, resolution, and selectivity compared to conventional one-dimensional LC. Its modular design supports gradient elution, heart-cutting, and comprehensive modulation modes—making it suitable for proteomics, metabolomics, natural product analysis, pharmaceutical impurity profiling, and environmental contaminant screening.
Key Features
- Two-mode pressure architecture: 120 MPa maximum for UHPLC applications (sub-2 µm particles), with selectable lower-pressure HPLC operation (60–70 MPa) for legacy column compatibility and method transfer.
- Digital linear motor-driven dual-piston pump: Delivers exceptional flow accuracy (≤0.06% RSD) and pulse-free solvent delivery across the full 0.001–10 mL/min range, critical for retention time stability and quantitative reproducibility.
- Intelligent autosampler with multi-vial tray support: Configurable carousel accommodating 330 × 1 mL, 192 × 2 mL, 108 × 4 mL, or 48 × 10 mL vials; injection volume programmable from 0.1 to 120 µL with syringe-based precision and low carryover (<0.005%).
- Thermostatically controlled column compartment: Maintains temperature from 10 °C below ambient to 80 °C with ±0.1 °C stability, ensuring retention time repeatability and method robustness across seasonal lab conditions.
- UV-Vis photodiode array detector (PDA): Standard 190–700 nm spectral coverage; optional extended-range configuration (190–900 nm) for broader compound detection including near-infrared-absorbing analytes.
- High-speed data acquisition: Up to 100 Hz sampling rate enables accurate peak shape characterization—even for narrow UHPLC peaks (<3 s width at base)—and supports real-time spectral deconvolution.
Sample Compatibility & Compliance
The EX1800 accommodates a broad spectrum of sample types—including small molecules, peptides, oligonucleotides, polar metabolites, and thermally labile compounds—when paired with appropriate stationary phases (C18, HILIC, SEC, chiral, etc.) and mobile phase chemistries. Its fluidic pathway employs biocompatible materials (e.g., PEEK, titanium, and ceramic components) to minimize adsorption and metal-catalyzed degradation. The system is designed to meet international regulatory expectations: software architecture supports audit trail, electronic signatures, and user access control per FDA 21 CFR Part 11; instrument qualification documentation aligns with IQ/OQ/PQ protocols under ISO/IEC 17025 and ICH Q2(R2); and hardware design facilitates GMP-compliant installation and maintenance practices.
Software & Data Management
The EX1800 is operated via Wufeng ChromaLink™, a validated chromatography data system (CDS) compliant with GLP/GMP workflows. It provides integrated method development tools—including gradient optimization, peak tracking, and retention modeling—as well as automated LC×LC modulation timing setup. Raw data are stored in vendor-neutral formats (e.g., mzML-compatible binary archives) and support third-party processing via open APIs. All user actions—including method edits, sequence changes, and report generation—are logged with timestamped, immutable audit trails. The software includes built-in compliance reports for 21 CFR Part 11 readiness assessment, electronic signature validation, and change control history review.
Applications
- Pharmaceutical QC/QA: Release testing of active pharmaceutical ingredients (APIs), excipients, and finished dosage forms per USP , EP 2.2.46, and JP 6.03.
- Biopharmaceutical characterization: Peptide mapping, glycan profiling, and aggregate analysis using reversed-phase and size-exclusion 2D-LC configurations.
- Food safety: Multi-residue pesticide screening, mycotoxin quantification, and adulterant detection in complex food matrices.
- Environmental analysis: Simultaneous determination of persistent organic pollutants (POPs), pharmaceutical residues, and microplastic leachates in water and soil extracts.
- Academic and industrial research: Method development for challenging separations requiring orthogonal selectivity—e.g., isomeric drug metabolites, natural product isomers, or synthetic polymer dispersity profiling.
FAQ
Does the EX1800 support both heart-cutting and comprehensive 2D-LC modes?
Yes—the system includes a high-speed, low-volume 6-port/2-position switching valve with configurable dwell volume, enabling both targeted heart-cutting and full LC×LC modulation with adjustable modulation periods.
Is the software qualified for regulated environments?
ChromaLink™ is delivered with documented IQ/OQ protocols, 21 CFR Part 11 configuration settings, and a validated electronic signature workflow compliant with GxP requirements.
Can the EX1800 be integrated into existing LIMS or ELN systems?
Yes—it supports ASTM E1384-compliant data export, RESTful API access for metadata exchange, and direct integration with major LIMS vendors via standard CSV, XML, or HL7 interfaces.
What column oven options are available?
The standard oven supports single-column or dual-column configurations with independent temperature zones; optional pre-heater/cooler modules are available for precise mobile-phase thermal management.
Is service and technical support available outside China?
Wufeng maintains certified service partners in North America, Europe, and APAC regions, with remote diagnostics, on-site calibration, and application support provided under annual service agreements.
