Wufeng LC-100P Preparative Liquid Chromatography System
| Brand | Wufeng |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | LC-100P |
| Price Range | USD 14,000–28,000 (FOB Shanghai) |
| Application Level | Laboratory-Scale Purification |
| Instrument Type | High-Pressure Preparative HPLC System |
| Flow Rate Range | 0.01–120 mL/min |
| Flow Accuracy | ±2% of set value |
| Flow Precision (RSD) | ≤0.2% |
| Maximum Operating Pressure | 42 MPa |
| Wavelength Range | 190–680 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | 0.1 nm |
| Baseline Noise | 2 × 10⁻⁵ AU (at 254 nm, 50 mL/min, methanol) |
| Baseline Drift | 2 × 10⁻⁴ AU/h (at 254 nm, 50 mL/min, methanol) |
| Data Acquisition Rate | 20 Hz |
| Communication Interface | RS-232 or USB |
| GLP Compliance Features | Deuterium lamp usage time logging, real-time lamp hour tracking, audit-ready operation logs |
Overview
The Wufeng LC-100P Preparative Liquid Chromatography System is an engineered high-pressure platform designed for laboratory-scale compound isolation, purification, and fraction collection in research and development environments. Based on the principles of reversed-phase and normal-phase liquid chromatography, the LC-100P delivers precise gradient and isocratic elution control across a wide flow range (0.01–120 mL/min) and pressure envelope (up to 42 MPa), enabling robust separation of small molecules, natural products, synthetic intermediates, and early-stage pharmaceutical candidates. Its modular architecture supports dual pump configurations—P40 semi-preparative and P120 preparative pumps—and UV40/UV120 detection modules, allowing users to configure either isocratic, binary high-pressure gradient, or multi-solvent systems without hardware retrofitting. The system operates under standard HPLC thermodynamic and fluidic principles, with laminar flow control, pulse-dampened solvent delivery, and real-time pressure monitoring ensuring reproducible retention times and peak resolution critical for downstream characterization and structural validation.
Key Features
- Modular high-pressure pump design: Interchangeable P40 (0.01–40 mL/min, 25 MPa max) and P120 (0.1–120 mL/min, 42 MPa max) units support both semi-preparative and full preparative workflows.
- UV detection with spectral fidelity: UV40 and UV120 detectors cover 190–680 nm with wavelength accuracy ±1 nm and repeatability of 0.1 nm—validated per ISO 17025 traceable calibration protocols.
- Low-noise optical path: Baseline noise ≤2 × 10⁻⁵ AU (254 nm, methanol, 50 mL/min) ensures high signal-to-noise ratio for trace-level fraction detection and automated peak triggering.
- Dual communication interface: RS-232 serial and USB 2.0 ports enable seamless integration with third-party data acquisition software and LIMS environments.
- GLP-compliant operational logging: Built-in deuterium lamp hour counter, timestamped method execution records, and user-accessible audit trails align with FDA 21 CFR Part 11 requirements for electronic records and signatures.
- Thermally stabilized flow path: Integrated heat dissipation and low-compressibility fluidics minimize retention time drift during extended runs (>8 h).
Sample Compatibility & Compliance
The LC-100P accommodates a broad spectrum of sample matrices—including crude plant extracts, reaction mixtures, peptide digests, polymer fractions, and API intermediates—when paired with standard stainless-steel or biocompatible PEEK-lined columns (20–50 mm ID, 100–250 mm length). Column compatibility extends to C18, C8, phenyl-hexyl, silica, amino, and cyano stationary phases. All wetted components meet USP Class VI biocompatibility standards. System compliance includes adherence to IEC 61010-1 (safety of laboratory equipment), ISO/IEC 17025:2017 (for calibration traceability of UV wavelength and flow verification), and ASTM D7212 (for UV-Vis detector performance validation). Optional IQ/OQ documentation packages are available for GMP-regulated labs requiring installation and operational qualification.
Software & Data Management
The LC-100P is controlled via Wufeng’s proprietary ChromSight™ PC-based software, supporting full bidirectional instrument control—including pump gradient programming, UV wavelength scanning, fraction collector triggering, and real-time chromatogram overlay. Data files are saved in vendor-neutral .csv and .dx formats, compatible with ChemStation, OpenLab CDS, and Python-based cheminformatics pipelines (e.g., PyChrom, MZmine). Software enforces role-based access control (admin/operator modes), electronic signature capture for method approval, and automatic backup of method parameters, run logs, and detector diagnostics. Audit trail functionality records all parameter changes, user logins, and method executions with immutable timestamps—meeting ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) data integrity criteria.
Applications
- Pharmaceutical R&D: Isolation of active pharmaceutical ingredients (APIs) from synthetic batches; purification of chiral intermediates using polysaccharide-based CSPs.
- Natural product chemistry: Fractionation of complex botanical extracts for NMR-guided structure elucidation; removal of chlorophyll and tannins prior to MS analysis.
- Chemical synthesis support: Recovery of catalysts, unreacted starting materials, and regioisomers from multi-step reactions.
- Food & beverage safety: Extraction and quantification of mycotoxins (e.g., aflatoxin B1), pesticides, and polyphenol standards.
- Environmental analysis: Cleanup of PAHs, PCBs, and flame retardants from soil and water extracts prior to GC-MS confirmation.
- Biochemistry: Desalting and buffer exchange of peptides post-solid-phase synthesis; purification of oligonucleotides and modified nucleosides.
FAQ
What column dimensions and particle sizes are recommended for optimal performance on the LC-100P?
Standard preparative columns ranging from 20 × 100 mm to 50 × 250 mm (ID × length) with 5–25 µm fully porous or superficially porous particles are supported. Particle size selection depends on flow rate and pressure constraints—e.g., 10 µm particles are typical for 50 mL/min at ≤20 MPa.
Does the LC-100P support pH-stable mobile phases?
Yes—the P120 pump head and UV120 flow cell are constructed from titanium alloy and sapphire windows, permitting long-term use of aqueous mobile phases from pH 1.5 to 12.0 when paired with compatible column hardware.
Can the system be integrated into an automated fraction collection workflow?
Yes—RS-232/USB TTL-level trigger outputs synchronize with third-party fraction collectors (e.g., Gilson FC204, Teledyne ISCO Foxy R1) based on UV threshold, time window, or peak apex detection.
Is method transfer from analytical to preparative scale feasible with this system?
Method translation is supported through Wufeng’s Scale-Up Calculator module in ChromSight™, which applies linear velocity preservation, constant loading mass scaling, and column volume normalization per USP guidelines.
What maintenance intervals are recommended for GLP-compliant operation?
Pump seals should be replaced every 6 months or after 1,000 hours of operation; UV lamp replacement is advised at 1,000 hours or when intensity drops >20% at 254 nm; system suitability testing (SST) must be performed before each batch sequence per internal SOP or regulatory requirement.
