Zymo Research EZ-Vac™ S7000 Vacuum Manifold for Plasmid Purification
| Origin | USA |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | S7000 |
| Price | USD 3,850 (FOB) |
Overview
The Zymo Research EZ-Vac™ S7000 Vacuum Manifold is an engineered vacuum-driven filtration platform specifically designed to replace time-intensive centrifugation steps in high-yield plasmid DNA purification workflows. It operates on the principle of controlled negative pressure (–20 to –30 inHg), enabling simultaneous vacuum-assisted binding, washing, and elution across up to 20 spin columns in parallel. Unlike conventional benchtop centrifuges, the S7000 eliminates rotor imbalance risks, reduces hands-on time by >70% during wash cycles, and ensures consistent flow-through kinetics critical for endotoxin-free plasmid recovery. Its architecture supports scalable preparation of transfection-grade plasmid DNA (up to 1 mg per prep) with reproducible A260/A280 ratios ≥1.8 and endotoxin levels <0.1 EU/µg — meeting ISO 13485-aligned quality thresholds for preclinical vector manufacturing.
Key Features
- Parallel processing capacity for 20 spin columns per cycle, eliminating sequential centrifugation bottlenecks
- Integrated one-way Luer-lock stopcocks with silicone O-rings ensure leak-free vacuum integrity and prevent cross-contamination between ports
- Chemically resistant neoprene stopper and polypropylene manifold body compatible with ethanol, isopropanol, guanidine hydrochloride, and low-pH wash buffers
- Modular hose interface accepts standard 8-mm I.D. vacuum tubing; compatible with laboratory vacuum pumps (≥25 L/min flow rate, ≤10 mbar ultimate vacuum)
- Ergonomic low-profile design (12.5 × 20.3 × 8.9 cm) optimized for Class II biosafety cabinets and laminar flow hoods
- No electrical components or moving parts — maintenance-free operation with validated longevity exceeding 10,000 vacuum cycles
Sample Compatibility & Compliance
The S7000 is validated exclusively for use with Zymo Research’s spin-column-based nucleic acid purification kits, including the Zyppy™ Plasmid Maxiprep Kit (D4202), PureLink™ HiPure Plasmid Filter Maxiprep Kit (K2100-01), and Quick-Clean™ Endotoxin-Free Plasmid Kits. It supports input volumes up to 50 mL lysate per column and accommodates both standard (1.5 mL) and high-capacity (5 mL) collection tubes. The system complies with ISO 13485:2016 requirements for medical device-related ancillary equipment and meets GLP documentation standards when used with Zymo’s FDA-registered kit lot traceability system. While not a standalone medical device, its configuration supports 21 CFR Part 11-compliant data capture when integrated with Zymo’s ZYMOsoft™ LIMS interface module (optional).
Software & Data Management
As a hardware-only vacuum manifold, the S7000 does not incorporate embedded firmware or onboard software. However, it integrates seamlessly into regulated laboratory environments via Zymo Research’s optional ZYMOsoft™ Laboratory Information Management System (LIMS) adapter kit. This add-on enables electronic batch record generation, operator authentication logging, and audit-trail-enabled vacuum cycle timestamping — fulfilling ALCOA+ data integrity principles. All operational parameters (e.g., vacuum duration per step, total cycle count) are recorded externally using calibrated vacuum gauges and time-stamped logbooks per GMP Annex 11 guidance.
Applications
- High-throughput plasmid DNA isolation for CRISPR gRNA vector production
- Endotoxin-sensitive applications including mRNA vaccine template preparation and lentiviral vector manufacturing
- Scalable purification of large-insert BAC/PAC clones (>150 kb) requiring extended wash stringency
- Quality control labs performing QC release testing per USP and Ph. Eur. 5.2.12
- Academic core facilities supporting >50 users/month with standardized SOPs for nucleic acid extraction
FAQ
Is the S7000 compatible with non-Zymo spin columns?
No. Use with third-party columns voids warranty and may cause seal failure, column rupture, or buffer leakage due to non-standard flange geometry and membrane burst pressure tolerances.
What vacuum source specifications are required?
A diaphragm or oil-free rotary vane pump delivering ≥25 L/min at ≤10 mbar (7.5 torr) is mandatory. Water aspirators are not recommended due to inconsistent vacuum profiles and potential backflow contamination.
Can the S7000 be sterilized?
The manifold body and stopper are autoclavable at 121°C for 20 minutes (gravity cycle only). Stopcocks must be disassembled and sterilized separately via ethylene oxide or gamma irradiation — steam exposure degrades internal silicone seals.
Does the system include vacuum gauge calibration certification?
No. Calibration certificates for external vacuum gauges (e.g., MKS Baratron® series) must be obtained from accredited metrology providers per ISO/IEC 17025:2017.
How is user training delivered?
Zymo Research provides on-demand video SOPs, PDF protocol supplements, and biannual virtual technical workshops — all accessible via the Zymo Support Portal (support.zymoresearch.com) without license fees.
