Allsheng Auto-Pure S32 Automated Nucleic Acid Extraction System
| Brand | Allsheng |
|---|---|
| Origin | Zhejiang, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | Auto-Pure S32 |
| Pricing | Upon Request |
| Principle | Magnetic Bead-Based Separation |
| Sample Throughput | 1–32 samples per run |
| Compatible Plate Format | 96-Deep-Well Plate |
| Processing Volume | 30–1000 µL |
| Inter-Well CV | ≤5% |
| Lysis Temperature Range | Ambient to 120°C |
| Elution Temperature Range | Ambient to 120°C |
| Heating Time (RT to 120°C) | ≤4 min |
| Cooling Time (100°C to 40°C) | ≤10 min (at 25°C ambient) |
| Temperature Accuracy | ±0.5°C |
| Temperature Uniformity | ±1°C at 100°C |
| Mixing Intensity | 10 Adjustable Levels |
| Display | 7-inch Color Touchscreen |
| Onboard Program Storage | Up to 500 Protocols |
| Interface Ports | 2× USB-A, 1× USB-B, 1× Ethernet |
| Wireless Connectivity | Built-in Wi-Fi (4G optional) |
| Barcode Scanning | External Scanner Support with Auto-Protocol/ID Recognition |
| UV Sterilization | Integrated Germicidal UV Lamp |
| Air Filtration | HEPA-Grade Aerosol Filtration System |
| Power Supply | AC 110–240 V, 50/60 Hz, 450 VA |
| Dimensions (W×D×H) | 417 × 410 × 426 mm |
| Weight | 20 kg |
Overview
The Allsheng Auto-Pure S32 Automated Nucleic Acid Extraction System is an engineered platform for high-fidelity, walk-away purification of DNA, RNA, and proteins from diverse biological matrices—including whole blood, serum, plasma, saliva, buccal swabs, cultured cells, and homogenized animal or plant tissues. It operates on the well-established magnetic bead separation principle: paramagnetic beads functionalized with silica-based surface chemistry selectively bind nucleic acids under controlled chaotropic salt and pH conditions. The system employs a precision-engineered magnetic rod array with disposable magnetic sleeves to aspirate, transfer, wash, and elute bead-bound nucleic acids—eliminating liquid handling errors associated with pipetting-based automation. Designed for clinical molecular laboratories, core facilities, and translational research environments, the Auto-Pure S32 delivers consistent yield and purity across variable sample types while maintaining strict compliance with pre-analytical workflow integrity requirements.
Key Features
- Flexible throughput: Processes 1–32 samples in parallel using standard 96-deep-well plates—enabling scalable batch processing without protocol revalidation.
- Thermally optimized lysis and elution modules: Independent heating zones support precise temperature control from ambient to 120°C, with ±0.5°C accuracy and ±1°C uniformity at 100°C—critical for efficient proteinase K digestion and low-volume elution recovery.
- Programmable mechanical agitation: Ten-step mixing intensity control allows fine-tuning of bead suspension kinetics during binding, washing, and elution—minimizing shear-induced fragmentation and maximizing binding efficiency.
- Integrated contamination mitigation: Dual-layer protection includes germicidal UV-C irradiation (254 nm) between runs and a HEPA-filtered laminar airflow path with positive-pressure exhaust—reducing aerosol-mediated cross-contamination risk in high-throughput settings.
- Human-centered interface: A responsive 7-inch capacitive touchscreen supports bilingual (English/Chinese) operation; optional external mouse input and barcode scanner integration enable hands-free protocol selection and sample tracking.
- Robust connectivity architecture: Native Wi-Fi and Ethernet interfaces support remote monitoring, centralized protocol deployment, and audit-ready logging—compatible with laboratory information management systems (LIMS) via standard TCP/IP protocols.
Sample Compatibility & Compliance
The Auto-Pure S32 accommodates a broad range of primary and processed biospecimens, including EDTA/K2EDTA anticoagulated blood, dried blood spots, FFPE tissue lysates, bacterial cultures, and viral transport media. Its open reagent design permits seamless integration with third-party magnetic bead kits validated under ISO 20387:2018 (biobanking) and CLSI MM19-A (nucleic acid extraction performance evaluation). The system’s thermal and mixing parameters are aligned with common manufacturer-specified lysis conditions for kits compliant with ISO 15189 and CAP-accredited workflows. All onboard program logs—including timestamped temperature profiles, mixing cycles, and UV activation events—are stored locally with immutable timestamps, supporting GLP/GMP documentation practices and FDA 21 CFR Part 11-compliant electronic record retention when paired with validated network storage solutions.
Software & Data Management
The embedded firmware supports up to 500 user-defined extraction protocols, each storing complete thermal ramping profiles, mixing schedules, magnetic actuation sequences, and incubation durations. Protocol import/export is supported via USB-A ports using encrypted .apx file format. Remote access is enabled through a secure web-based dashboard accessible via Android tablets or desktop browsers—displaying real-time instrument status, queue management, and historical run analytics. Audit trails capture operator ID (via optional RFID badge reader), start/stop times, parameter modifications, and error codes with SHA-256 hashing for data integrity verification. Optional cloud synchronization enables federated lab management across multi-site deployments, with role-based permissions and automated backup to NAS or AWS S3-compatible repositories.
Applications
- Clinical diagnostics: High-yield isolation of cell-free DNA (cfDNA) from plasma for oncology liquid biopsy workflows.
- Infectious disease testing: Rapid extraction of viral RNA from nasopharyngeal swabs compatible with RT-qPCR and next-generation sequencing library prep.
- Biobanking: Standardized processing of longitudinal human biospecimens under ISO 20387-aligned SOPs.
- Plant and animal genomics: Efficient recovery of high-molecular-weight DNA from fibrous or polysaccharide-rich tissues using customized lysis buffer presets.
- Quality control: Routine verification of extraction efficiency using spike-in controls (e.g., MS2 phage RNA) and spectrophotometric (A260/A280) or fluorometric quantification.
FAQ
What types of magnetic bead kits are compatible with the Auto-Pure S32?
The system accepts all commercially available 96-well magnetic bead kits designed for deep-well plate formats—including those from QIAGEN, Thermo Fisher Scientific, Takara Bio, and domestic suppliers—provided they specify compatibility with automated magnetic rod-based platforms.
Does the Auto-Pure S32 support unattended overnight operation?
Yes—its thermally stable architecture, low-power standby mode, and automatic UV sterilization cycle enable fully autonomous batch processing with no manual intervention required between runs.
How is data integrity ensured during network transmission?
All remote commands and log exports use TLS 1.2 encryption; locally stored audit trails are digitally signed and tamper-evident, meeting ALCOA+ principles for regulated environments.
Can the instrument be integrated into a LIMS environment?
It supports HL7 and ASTM E1384-compliant message exchange via Ethernet or Wi-Fi, enabling bidirectional sample ID synchronization and result auto-import upon completion.
What maintenance is required for long-term reliability?
Routine tasks include weekly UV lamp output verification, monthly magnetic sleeve wear inspection, and quarterly calibration of temperature sensors using NIST-traceable reference probes—documented in the included maintenance logbook.
