LUMEX Capel 205 High-Performance Capillary Electrophoresis System

Overview

The LUMEX Capel 205 is a high-performance, research-grade capillary electrophoresis (CE) system engineered for precision, reproducibility, and operational flexibility in regulated and academic laboratories. It operates on the fundamental principle of electrophoretic mobility separation—where analytes migrate under the influence of a high-voltage electric field through a fused-silica capillary filled with electrolyte buffer. The system integrates advanced thermal management, programmable hydrodynamic and electrokinetic injection, and high-fidelity UV-Vis detection to deliver robust resolution of small ions, organic acids, amino acids, pharmaceuticals, chiral compounds, and biomolecules. Designed for compliance-driven environments, the Capel 205 supports full traceability per FDA 21 CFR Part 11 requirements—including electronic signatures, secure user roles, and immutable audit trails within its Elforun-205 software platform.

Key Features

• Patented SPC (Surface Potential Control) technology actively restores capillary wall zeta potential prior to each run, minimizing memory effects and improving migration time reproducibility (<0.5% RSD over 100 injections).
• Liquid-cooled capillary jacket with ±0.1 °C temperature stability across -10 °C to +65 °C—enabling high-resolution separations of thermally labile or aggregation-prone species without air-cooling artifacts.
• High-voltage module delivering ±30 kV at 0.1 kV resolution and automatic polarity switching, coupled with current monitoring up to 300 µA and power output up to 9 W for rapid, high-efficiency separations.
• Triple-mode injection system: voltage (±30 kV), pressure (±2000 mbar), and reverse vacuum—enabling ultra-fast (<60 s) analyses and online sample stacking for sub-ppb detection limits.
• 59-position autosampler with Eppendorf 2 mL tube compatibility and automated cap removal; maintains sample integrity via sealed vial handling and eliminates evaporation or cross-contamination.
• Optimized optical path featuring xenon flash lamp (190–600 nm), fixed-pathlength flow cell, and real-time spectral scanning—achieving detection limits down to 0.1 ng/mL for common anions and cations.

Sample Compatibility & Compliance

The Capel 205 accommodates a broad spectrum of aqueous and semi-aqueous matrices without derivatization, including environmental waters (EPA 6500), food extracts (OIV, GB/T 30921.1-2014), biological fluids, pharmaceutical intermediates, and forensic residues. It supports all major CE separation modes: capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), capillary isoelectric focusing (CIEF), and capillary gel electrophoresis (CGE). Method validation documentation aligns with ISO/IEC 17025, ASTM D6508-00, ASTM D7881/D7882, and USP <1053>. Instrument qualification packages include IQ/OQ protocols compliant with GMP/GLP frameworks, and raw data files are stored in vendor-neutral .csv and .cef formats for third-party review.

Software & Data Management

Elforun-205 is a Windows-based, 64-bit application providing full instrument control, real-time electropherogram visualization, peak integration with baseline correction algorithms, and spectral deconvolution. The software includes over 120 pre-validated application methods—covering anion/cation analysis in drinking water, organic acid profiling in beverages, amino acid quantification in dairy, and antibiotic screening in feed. All method parameters (voltage ramping, temperature gradients, detection wavelength sweeps) are fully scriptable. Audit trails log every user action with timestamps, IP addresses, and reason-for-change fields. Export options include PDF reports with embedded chromatograms, Excel-compatible tables, and XML metadata for LIMS integration.

Applications

The Capel 205 is routinely deployed in food safety labs for simultaneous quantification of preservatives (sorbate, benzoate), sweeteners (aspartame, sucralose), and anions (NO₃⁻, SO₄²⁻) in soft drinks and wines. Environmental laboratories apply it to EPA-compliant speciation of F⁻, Cl⁻, Br⁻, NO₂⁻, PO₄³⁻, and NH₄⁺ in groundwater and wastewater. In biopharmaceutical QC, it resolves enantiomeric impurities in chiral APIs and monitors charge variants of monoclonal antibodies via CIEF. Forensic toxicology units use its high-speed vacuum injection mode for rapid screening of explosives residues (e.g., nitroglycerin, RDX) and alkaloid toxins (strychnine, nicotine) in swab extracts. Agricultural testing facilities rely on its MEKC capability for pesticide metabolite profiling in soil leachates.

FAQ

Does the Capel 205 support method transfer from other CE platforms?
Yes—method parameters (voltage, temperature, buffer composition, capillary dimensions) are exportable as standardized .cef files and can be imported into Elforun-205 with minimal re-optimization.
Is the liquid cooling system maintenance-free?
The closed-loop deionized water circulation requires only annual coolant replacement and periodic pump inspection; no refrigerant handling or external chillers are needed.
Can the system perform unattended overnight runs?
With the 59-position autosampler and integrated method sequencing, up to 5,900 injections can be queued—including automated capillary rinsing, buffer replenishment, and data archiving—with no manual intervention.
What regulatory documentation is provided with shipment?
Each unit ships with Factory Acceptance Test (FAT) report, Certificate of Conformance, IQ/OQ templates aligned with Annex 11 and ALCOA+ principles, and a complete set of software validation documents.
Is remote diagnostics supported?
Elforun-205 includes secure TLS-encrypted remote access (with customer-configurable firewall rules) for technical support teams to monitor instrument health, retrieve error logs, and assist with method troubleshooting without compromising data security.