Zhicheng ZWY-2112B Dual-Layer High-Capacity Full-Temperature Incubated Orbital Shaker

Overview

The Zhicheng ZWY-2112B is a dual-layer, high-capacity incubated orbital shaker engineered for demanding life science and bioprocess applications requiring precise temperature control, stable oscillation, and scalable culture volume. It operates on the principle of orbital motion—generating uniform circular agitation to promote homogeneous oxygen transfer, suspension culture mixing, and consistent thermal distribution across large-volume vessels. Its full-temperature range (4–60 °C), combined with forced-air convection and PID-controlled microprocessor regulation, ensures tight thermal stability (≤ ±0.1 °C at 37 °C) and uniformity (≤ ±1.0 °C), critical for sensitive microbial fermentations, mammalian cell expansion, and enzymatic assays. Designed specifically for GMP-aligned environments—including pilot-scale bioreactor inoculum preparation, pharmaceutical upstream processing, and blood bank storage conditioning—the ZWY-2112B integrates structural rigidity, operational safety redundancy, and regulatory-ready functionality without compromising accessibility or serviceability.

Key Features

• Dual-tier stainless-steel shelving system (2 × 970 × 560 mm) supporting up to 582 L total chamber volume and maximum load configurations including 1000 mL flasks × 36 per shelf
• PID-based environmental scanning microprocessor controller with real-time feedback loop for temperature and speed regulation
• Backlit LCD display showing setpoints and actual values for temperature (°C), speed (rpm), and remaining time (h)
• Programmable timer with 500-hour capacity; automatic shutdown with audible/visual alarm upon completion or fault detection
• AC induction motor with brushless design, delivering constant torque across 30–300 rpm range and rated for >20,000 hours MTBF
• Triple-stage safety protocol: independent overtemperature cutoff (with thermal fuse backup), motor overheating protection, and power-failure recovery with auto-resume capability
• Soft-start acceleration circuitry minimizing mechanical shock to culture vessels during startup and speed transitions
• Ergonomic dual-front door access with tempered glass viewport and electrostatically coated steel enclosure for corrosion resistance and cleanroom compatibility

Sample Compatibility & Compliance

The ZWY-2112B accommodates standard laboratory vessels—including Erlenmeyer flasks (100–1000 mL), baffled bottles, multi-well plates (with optional adapters), and custom bioreactor bags—on its reinforced, non-slip aluminum-alloy shelves. Its orbital motion (Φ35 mm) provides gentle yet effective mixing suitable for aerobic bacterial cultures (e.g., E. coli, Bacillus spp.), yeast fermentations (Saccharomyces cerevisiae), insect and CHO cell lines, and temperature-sensitive hybridization reactions. The unit complies with IEC 61010-1:2010 for laboratory equipment safety and meets electromagnetic compatibility (EMC) requirements per EN 61326-1. While not pre-certified for FDA 21 CFR Part 11, its parameter-locking function, audit-trail-capable logging (via optional RS485/USB interface), and configurable user access levels support validation under GLP and GMP frameworks when integrated into controlled documentation systems.

Software & Data Management

Local operation is fully managed via the embedded microcontroller interface, which supports password-protected parameter locking, multi-user profile storage (up to 10 protocols), and persistent memory retention for all active settings—even during power interruption. Optional communication modules enable connection to external SCADA or LIMS platforms using Modbus RTU or ASCII protocols. When paired with Zhicheng’s optional PC-based software suite (v3.2+), users gain access to real-time remote monitoring, automated data export in CSV/Excel format, trend graphing of temperature and speed profiles, and electronic signature-enabled report generation compliant with ALCOA+ data integrity principles. All logged events—including alarms, door openings, setpoint changes, and runtime cycles—are timestamped and stored onboard for ≥30 days.

Applications

• Pharmaceutical upstream processing: seed train expansion, media optimization studies, and stability-indicating shake flask screening
• Clinical diagnostics: blood bag agitation for platelet storage (per AABB standards), serum separation protocols, and antigen-antibody incubation
• Academic and industrial microbiology: high-yield antibiotic fermentation, recombinant protein expression, and synthetic biology chassis characterization
• Food safety testing: enrichment cultures for Salmonella, Listeria, and Campylobacter per ISO 6579 and FDA BAM guidelines
• Environmental microbiology: BOD bottle shaking, activated sludge respirometry, and biodegradation kinetic assays
• Molecular biology: plasmid propagation, phage amplification, and chromatin immunoprecipitation (ChIP) prep workflows

FAQ

What is the minimum ambient temperature required for refrigerated operation?
The unit requires an ambient environment between 5 °C and 25 °C for stable sub-ambient temperature maintenance. Operation below 5 °C ambient may impair compressor efficiency and condensate management.
Can the ZWY-2112B maintain 4 °C continuously with full load?
Yes—under standard laboratory conditions (22 °C ambient, 50% RH), the system achieves and sustains 4 °C with ≤±0.3 °C deviation across the chamber when loaded per manufacturer-specified vessel distribution guidelines.
Is validation documentation (IQ/OQ/PQ) available?
Zhicheng provides standardized IQ/OQ templates and factory test reports (including temperature mapping at three load conditions). PQ execution requires site-specific protocol development aligned with internal quality systems.
Does the shaker support programmable ramping between temperature/speed setpoints?
No—ramping is not natively supported. However, sequential protocols can be manually staged using the timer and parameter recall functions, or automated externally via Modbus commands.
What maintenance intervals are recommended?
Biannual inspection of door seals, fan filters, and refrigerant pressure; annual calibration verification of temperature and speed sensors using NIST-traceable references; no routine lubrication required due to sealed motor and bearing assembly.