Capel-205 High-Efficiency Capillary Electrophoresis System

Overview

The Capel-205 High-Efficiency Capillary Electrophoresis System is a robust, benchtop analytical platform engineered for high-resolution separation and quantitative analysis of ionic and zwitterionic analytes—including small molecules, peptides, proteins, nucleic acids, and chiral compounds. It operates on the fundamental principle of capillary electrophoresis (CE), wherein analytes migrate through a fused-silica capillary under the influence of a high-voltage electric field (typically up to ±30 kV), with separation governed by differences in charge-to-size ratio and electroosmotic flow (EOF). Detection is performed via UV absorbance at selectable wavelengths (190–400 nm) using a high-stability optical path and temperature-controlled flow cell, ensuring baseline stability and signal reproducibility. The system integrates a precision liquid thermostat (±0.1 °C control over 15–60 °C range) to minimize Joule heating effects and maintain migration time repeatability—critical for method transfer and regulatory compliance.

Key Features

• Integrated high-voltage power supply with programmable voltage ramping and current limiting for safe, stable operation across diverse buffer systems
• Temperature-regulated capillary cartridge housing with real-time monitoring and PID-controlled liquid circulation
• Autosampler with 96-well plate compatibility, precise syringe-driven injection (pressure or vacuum mode), and programmable rinse cycles to prevent carryover
• Modular optical detection unit featuring deuterium lamp source, holographic grating monochromator, and low-noise photodiode array detector
• Full PC-based control via dedicated CE acquisition software supporting method development, sequence definition, and real-time electropherogram visualization
• Rugged mechanical design with electromagnetic shielding and grounded chassis to ensure signal integrity in shared laboratory environments

Sample Compatibility & Compliance

The Capel-205 accommodates a broad spectrum of sample matrices—ranging from biological fluids (serum, urine, cell lysates) and pharmaceutical formulations to environmental extracts and food additives—provided they are soluble in aqueous or mixed organic-aqueous electrolyte buffers. Capillary dimensions (standard 50–100 µm ID × 30–100 cm total length) support both uncoated and dynamically or covalently coated capillaries for optimized selectivity. The system conforms to core CE operational requirements outlined in ICH Q5A(R2), USP , and ISO/IEC 17025:2017 for analytical method validation. Data acquisition and audit trail functionality align with GLP and GMP documentation expectations; raw data files are stored in vendor-neutral formats (e.g., .cef) compatible with third-party processing tools.

Software & Data Management

Control and data handling are managed through CE-Studio software, a Windows-native application compliant with FDA 21 CFR Part 11 requirements when deployed with appropriate IT infrastructure (user authentication, electronic signatures, and immutable audit trails). The software supports multi-channel method optimization—including voltage, temperature, injection parameters, and detection wavelength—and enables automated peak integration using customizable baseline algorithms (tangent skim, valley-to-valley, or second-derivative detection). Export options include CSV, PDF, and XML for LIMS integration. All method files and electropherograms are timestamped and digitally signed, with version history retained for traceability during internal audits or regulatory inspections.

Applications

• Pharmaceutical quality control: Assay of active pharmaceutical ingredients (APIs), counterion profiling, and chiral purity assessment per USP monographs
• Biomolecular characterization: SDS-CGE for protein purity, cIEF for pI determination, and CGE for oligonucleotide size analysis
• Clinical diagnostics: Hemoglobin variant screening (HbA1c, HbS), serum protein electrophoresis (SPEP), and therapeutic drug monitoring
• Food safety testing: Detection of banned dyes, preservatives, and mycotoxins at sub-ppm levels
• Environmental analysis: Anion/cation profiling in wastewater and soil leachates using CZE and CEC modes

FAQ

What capillary types are supported by the Capel-205?
Standard fused-silica capillaries (50–100 µm ID, 360 µm OD) with polyimide coating; both bare silica and commercially available coated capillaries (e.g., neutral-coated, polyacrylamide, or cellulose derivatives) are compatible.
Does the system support multiple detection modes?
The Capel-205 is configured for UV-Vis absorbance detection only; fluorescence or contactless conductivity detection require external module integration and are not natively supported.
Is method validation documentation provided?
Yes—the system delivery includes IQ/OQ protocols, a factory calibration report, and a comprehensive user manual with SOP templates aligned with ISO/IEC 17025 and USP .
Can data be exported to LIMS without proprietary software?
Raw electropherograms (.cef) and processed results (.csv, .pdf) are exportable without runtime dependency on CE-Studio; no license dongle or background service is required for file access.
What maintenance is required for long-term reliability?
Routine maintenance includes daily capillary flushing with NaOH and water, quarterly replacement of electrode buffer solutions, and annual verification of high-voltage insulation resistance and optical alignment per manufacturer guidelines.