LY DYCZ-20A DNA Sequencing Electrophoresis Apparatus
| Brand | LY |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | DYCZ-20A |
| Instrument Type | Nucleic Acid Electrophoresis System |
| Main Unit | Electrophoresis Chamber with Integrated Tank |
| Gel Tray Dimensions | 580 × 170 mm |
| Overall Dimensions (L × W × H) | 240 × 210 × 655 mm |
| Buffer Capacity | 850 mL |
| Sample Comb Options | 32-tooth “Shark-Tooth” and 26-tooth “Great Wall” combs, 0.4 mm thickness |
| Weight | 10.5 kg |
Overview
The LY DYCZ-20A DNA Sequencing Electrophoresis Apparatus is a vertical slab gel electrophoresis system engineered for high-resolution nucleic acid separation in molecular biology laboratories. It operates on the principle of electrophoretic mobility under a constant electric field, where DNA fragments migrate through a polyacrylamide or agarose matrix according to size and charge-to-mass ratio. Designed specifically for DNA sequencing applications—including Sanger-based chain-termination analysis—the DYCZ-20A supports denaturing and non-denaturing conditions with thermal stability critical for reproducible band resolution. Its vertical configuration enables precise control over gel polymerization, uniform electric field distribution, and minimal buffer convection—key factors influencing band sharpness and inter-gel repeatability. The system is intended for use with compatible power supplies such as the LY DYY-10C, DYY-12, DYY-12C, DYY-12D, and DYY-15D series, ensuring compliance with standard laboratory voltage and current requirements (up to 600 V / 100 mA typical range).
Key Features
- Stable vertical electrophoresis chamber with integrated lower buffer tank and optimized electrode alignment for uniform field strength across the full gel surface
- Enhanced thermal management via passive convection design and strategically placed buffer reservoirs, maintaining consistent gel temperature during extended runs (e.g., 4–16 h sequencing gels)
- Dual comb compatibility: includes both 32-tooth “Shark-Tooth” and 26-tooth “Great Wall” sample combs (0.4 mm thickness), supporting variable loading capacity and ladder resolution
- Integrated buffer drain port at the base of the lower tank for rapid, spill-free buffer exchange without disassembly
- Modular gel-casting system featuring two concave and two flat glass plates, silicone gaskets, sealing clamps (12 pcs), and adjustable spacers—enabling repeatable 0.4 mm gel thickness with minimal leakage
- Ergonomic gel pouring aid: supplied with calibrated 600 mL silicone tubing and dedicated gel-pouring bottle (160-0021) for bubble-free acrylamide layering
Sample Compatibility & Compliance
The DYCZ-20A accommodates standard sequencing-grade polyacrylamide gels (e.g., 6–10% T, 2.6–5% C) and high-percentage denaturing gels (e.g., 8 M urea). It supports common nucleic acid applications including dideoxy sequencing, differential display PCR, SSCP (Single-Strand Conformation Polymorphism), and DNA fingerprinting. All wetted components are chemically resistant to acrylamide monomers, TEMED, APS, and common electrophoresis buffers (TBE, TAE, EDTA-containing formulations). While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 by default, the apparatus meets general laboratory safety standards for Class I electrical equipment (IEC 61010-1) and is suitable for GLP-compliant workflows when operated within validated SOPs and paired with traceable power supplies and documentation protocols.
Software & Data Management
The DYCZ-20A is a hardware-only electrophoresis platform and does not include embedded firmware or proprietary software. Data acquisition and analysis are performed externally using industry-standard imaging systems (e.g., UV transilluminators, CCD-based gel documentation stations) and open-format analysis tools such as ImageJ, Quantity One (Bio-Rad), or AlphaView SA (ProteinSimple). Users may integrate run parameters—including voltage, time, buffer composition, and ambient lab temperature—into LIMS or ELN systems manually or via CSV export from auxiliary power supply units. For audit-trail compliance, laboratories are advised to record gel casting date, polymerization time, loading volume, and post-run staining protocol per batch in accordance with internal QA/QC procedures.
Applications
- DNA sequencing via manual or automated Sanger methods requiring high-resolution separation of fragments differing by single nucleotides
- SSCP analysis for detection of point mutations in clinical or population genetics studies
- Differential display profiling of cDNA expression patterns under varying physiological conditions
- Forensic DNA fingerprinting using multi-locus VNTR or STR markers
- Verification of restriction digest completeness and PCR product integrity prior to cloning or NGS library preparation
- Quality control of synthetic oligonucleotides and gene fragments in contract research and biomanufacturing settings
FAQ
Is the DYCZ-20A compatible with automated gel loaders or robotic liquid handlers?
No—it is a manual casting and loading system; integration with automation requires third-party adapters or custom fixture design.
What is the maximum recommended run time for continuous operation?
Up to 16 hours at ≤400 V and ambient temperatures below 25 °C; extended runs require active cooling or external chillers.
Can this unit be used for protein SDS-PAGE?
Yes, with appropriate glass plate spacing (0.75–1.5 mm) and modified comb selection; however, the standard 0.4 mm combs are optimized for nucleic acids.
Does LY provide calibration certificates or metrological traceability for this model?
No—calibration is user-performed via reference DNA ladders (e.g., pBR322/HaeIII, φX174/HaeIII); third-party verification services may be engaged separately.
Are replacement parts available globally through authorized LY distributors?
Yes—glass plates, silicone gaskets, combs, and clamps are stocked under standardized part numbers (e.g., 142-2011, 144-0040) and distributed via LY’s international partner network.
