2mag MIX 1 Cell Culture Magnetic Stirrer
| Brand | 2mag |
|---|---|
| Origin | Germany |
| Model | MIX 1 |
| Maximum Stirring Volume | 1000 L |
| Speed Range | 0–2000 rpm |
| Temperature Control Range | 0–200 °C |
| Instrument Type | Magnetic Stirrer |
| Warranty | 3 Years (Germany-manufactured) |
Overview
The 2mag MIX 1 Cell Culture Magnetic Stirrer is an engineered solution for precise, contamination-free agitation in sensitive biological applications—particularly mammalian and microbial cell culture under controlled environmental conditions. Unlike conventional stirrers relying on mechanical shafts or exposed drive mechanisms, the MIX 1 employs contactless magnetic coupling based on synchronous stepper motor technology. This principle eliminates seal wear, lubrication requirements, and particle generation—critical advantages for ISO Class 5–7 cleanroom environments, CO₂ incubators, and long-term sterile bioprocessing workflows. Designed and manufactured in Germany to DIN EN ISO 9001 standards, the device integrates robust electromagnetic field control with thermal stability across its full operating range (0–200 °C), enabling both ambient mixing and heated homogenization of aqueous, viscous, or serum-supplemented media.
Key Features
- True maintenance-free operation: No brushes, gears, or seals—stepper motor drive ensures zero particulate shedding and >50,000 hours MTBF.
- IP68-rated stainless steel housing: Fully submersible, autoclavable-compatible, and resistant to condensation, ethanol, and 70% isopropanol—validated for continuous use inside CO₂ incubators (5% CO₂, 37 °C, 95% RH).
- Wide dynamic speed range: 0–2000 rpm with closed-loop feedback control; programmable ramp profiles support gentle suspension of fragile adherent cells or high-shear dispersion of cryopreserved pellets.
- Thermal precision: Peltier-assisted heating plate delivers ±0.5 °C uniformity across surface (±1.0 °C at 200 °C), compliant with ASTM E2914-22 for laboratory hotplate performance verification.
- Scalable platform compatibility: Accepts standard borosilicate glass, polycarbonate, and PETG vessels from 1 mL vials to 1000 L bioreactor bags via configurable magnetic stir bars (e.g., PTFE-coated elliptical, encapsulated ceramic-core).
- EMC-certified design: Meets EN 61326-1:2013 for electromagnetic compatibility in shared lab environments with sensitive analytical instrumentation (e.g., mass spectrometers, patch-clamp rigs).
Sample Compatibility & Compliance
The MIX 1 supports sterility-critical workflows per ISO 13408-1 (aseptic processing) and USP . Its sealed architecture prevents ingress of airborne microorganisms during extended incubation cycles (>72 h). Compatible with USP Class VI-certified stir bars and single-use bioprocess containers (Sartorius BPC, Thermo Fisher HyPerforma). All wetted surfaces comply with FDA 21 CFR Part 11 requirements when paired with 2mag’s optional audit-trail-enabled software (MIXcontrol Suite v4.2). Device firmware conforms to IEC 62304 Class B for medical-grade embedded systems—making it suitable for GMP-compliant QC labs supporting cell therapy manufacturing (ATMPs).
Software & Data Management
When integrated with the optional MIXcontrol 20 controller or standalone USB interface, the MIX 1 logs timestamped speed, temperature, and runtime data to CSV/Excel formats. Full GLP/GMP traceability is enabled via user-access-level authentication, electronic signatures, and immutable audit trails meeting FDA 21 CFR Part 11 Annex 11 criteria. Remote monitoring via Ethernet or Wi-Fi (IEEE 802.11ac) allows integration into LabVantage or STARLIMS LIMS platforms. Firmware updates are digitally signed and validated against SHA-256 checksums to ensure integrity.
Applications
- Adherent and suspension cell culture in T-flasks, roller bottles, and wave bioreactors.
- Preparation of homogeneous monoclonal antibody feed stocks prior to tangential flow filtration.
- Uniform dispersion of hydrogels (e.g., Matrigel®, collagen I) for 3D organoid differentiation protocols.
- Stirring of high-viscosity fermentation broths (up to 15,000 cP) in benchtop bioreactors without vortexing or foaming.
- Long-term mixing of diagnostic reagent kits under accelerated stability testing (ICH Q1A).
- Environmental simulation studies requiring simultaneous pH/DO/speed correlation in aquatic toxicology assays.
FAQ
Can the MIX 1 operate continuously inside a CO₂ incubator without external cooling?
Yes—the IP68 enclosure and thermally isolated stepper motor allow uninterrupted 24/7 operation at 37 °C and 5% CO₂ without derating.
Is calibration required before GMP use?
Per ISO/IEC 17025, speed and temperature sensors are factory-calibrated with NIST-traceable references; users must perform periodic verification using accredited reference standards (e.g., Fluke 1523 with dry-well calibrator).
What stir bar sizes are recommended for 500 mL spinner flasks?
A 40 mm elliptical PTFE-coated stir bar (2mag part #SB-40EL-PTFE) provides optimal torque transfer and laminar flow profile for suspension cultures.
Does the device support RS-232 or Modbus RTU communication?
Yes—via optional serial interface module (2mag SKU: COM-RS232-MOD), enabling PLC integration for automated bioprocess lines.
How is sterility maintained during cleaning between runs?
The unit withstands repeated wipe-downs with sporicidal agents (e.g., 0.5% sodium hypochlorite) and steam sterilization at 121 °C for 15 min (validated per ISO 17665-1).
