Teledyne ISCO AccQ Prep HP150 High-Pressure Preparative Liquid Chromatography System

Overview

The Teledyne ISCO AccQ Prep HP150 is a high-pressure preparative liquid chromatography (PLC) system engineered for robust, scalable compound purification in research and development laboratories. Unlike analytical HPLC platforms optimized for detection sensitivity, the HP150 is purpose-built for throughput-driven isolation—delivering gram-to-multi-gram quantities of high-purity compounds under precisely controlled gradient or isocratic conditions. Its core architecture leverages positive-displacement dual-piston pumps to maintain exceptional flow stability across the full 1–150 mL/min range without mechanical pump-head swaps—a critical advantage for method transfer between scouting, optimization, and scale-up stages. The system operates at up to 6000 psi, enabling use of sub-10 µm particle-packed preparative columns (e.g., C18, phenyl-hexyl, silica) for improved resolution and loading capacity. Detection is performed via high-stability UV/Vis optics with 1 nm wavelength resolution and low baseline noise (<1 × 10⁻⁵ AU), ensuring reliable peak integration even for trace-level impurities co-eluting near major fractions.

Key Features

• Integrated touchscreen interface with PeakTrak® software—no external PC required; all method development, execution, and fraction collection managed via intuitive drag-and-drop workflow.
• Real-time solvent and waste reservoir monitoring with automatic shutdown to prevent overflow and minimize lab safety hazards.
• RFID-tagged fraction collector trays ensure unambiguous assignment of collected vials to chromatographic peaks—eliminating manual labeling errors during high-throughput runs.
• Modular design supports seamless integration of optional modules: auto-sampler (enabling unattended sequential injections), column selector valve (for automated switching among four columns with independent solvent compatibility), and auxiliary detectors (ELSD, mass spectrometer interface).
• Compact footprint (W × D × H: 61 × 66 × 53 cm) optimized for space-constrained labs while maintaining full high-pressure performance and thermal management integrity.
• Flow accuracy of RSD < 0.05% and reproducibility of RSD ≤ 1% meet stringent requirements for method validation under GLP and GMP-aligned workflows.

Sample Compatibility & Compliance

The AccQ Prep HP150 accommodates diverse sample matrices including natural product extracts, synthetic reaction mixtures, peptide libraries, and small-molecule APIs. It supports reversed-phase, normal-phase, and ion-exchange separations using standard 10–50 mm ID preparative columns. All fluidic pathways are constructed from chemically resistant materials (e.g., stainless steel, PEEK, sapphire) compatible with aggressive solvents (e.g., DCM, THF, TFA, high-acid/base mobile phases). The system complies with general laboratory safety standards (ANSI Z9.5, IEC 61010-1) and supports audit-trail functionality within PeakTrak® software—facilitating adherence to FDA 21 CFR Part 11 requirements when configured with user authentication and electronic signature modules. Method parameters, run logs, and fraction metadata are stored in encrypted, timestamped files suitable for regulatory submission.

Software & Data Management

PeakTrak® v5.x provides a unified, embedded environment for method creation, real-time chromatogram visualization, dynamic fraction triggering (based on threshold, time, or UV area), and post-run analysis. Fraction maps are automatically color-coded and overlaid on the chromatogram, with export options to CSV, PDF, and AIA (.cdf) formats for LIMS integration. Raw data files include full detector spectra (200–800 nm at 200 Hz), pressure traces, and flow profiles—enabling retrospective reintegration or spectral deconvolution. Software updates are delivered via secure HTTPS and validated per ISO/IEC 17025-compliant change control procedures.

Applications

• Purification of synthetic intermediates and final APIs prior to NMR, XRD, or biological assay testing.
• Isolation of bioactive constituents from plant, microbial, or marine extracts for structure–activity relationship (SAR) studies.
• Desalting and buffer exchange of peptides and oligonucleotides following solid-phase synthesis.
• Chiral separation of racemates using polysaccharide-based chiral columns at preparative scale.
• Process development support for downstream purification steps in biomanufacturing (e.g., mAb fragment polishing).

FAQ

Does the AccQ Prep HP150 require a dedicated computer for operation?

No—the system includes an integrated industrial-grade touchscreen running PeakTrak® software; no external PC is needed for routine operation or method development.
Can the system be validated for use in regulated environments?

Yes—when equipped with optional 21 CFR Part 11 compliance features (user roles, electronic signatures, audit trail), it supports IQ/OQ/PQ documentation frameworks aligned with pharmaceutical quality systems.
What column dimensions and chemistries are supported?

Standard preparative columns from 10 mm to 50 mm internal diameter, packed with C18, C8, phenyl, cyano, amino, diol, silica, or chiral stationary phases; maximum pressure rating must not exceed 6000 psi.
Is method transfer from analytical to preparative scale supported?

Yes—PeakTrak® includes scaling wizards that adjust flow rate, injection volume, and gradient slope based on column geometry and particle size, preserving retention and selectivity relationships.
How is fraction collection accuracy ensured across long unattended runs?

RFID identification of each collection rack, combined with real-time UV-triggered fraction cutting and hardware-level position feedback from the collector stepper motor, ensures positional fidelity within ±0.5 mL across 100+ fractions.