ANYAN CYFY-8 96-Well Plate Automated Peristaltic Microplate Dispenser

Overview

The ANYAN CYFY-8 96-Well Plate Automated Peristaltic Microplate Dispenser is an engineered solution for high-reproducibility liquid handling in mid-throughput life science laboratories. Unlike positive-displacement or air-displacement pipetting systems, the CYFY-8 employs a calibrated dual-peristaltic pump architecture—eight independent fluid paths driven by precision-rolled silicone tubing—to deliver non-contact, contamination-minimized dispensing across standard SBS-format 96-well microplates. Its operational principle relies on controlled occlusion of flexible tubing by rotating rollers, generating consistent volumetric displacement without internal valving or piston wear. This design eliminates carryover risk, enables rapid reconfiguration between reagent types, and ensures compatibility with chemically aggressive, viscous, or volatile liquids that challenge conventional syringe- or piston-based dispensers. The system is purpose-built for applications requiring traceable, repeatable distribution of assay reagents, cell culture media, standards, or QC samples—particularly where regulatory adherence (e.g., ISO/IEC 17025, CLIA, or FDA-aligned SOPs) mandates documented dispensing parameters and cleaning validation.

Key Features

• Eight-channel peristaltic dispensing head with independent roller control per channel, enabling parallel processing of up to eight identical or differentiated reagents in a single run.
• Dispensing accuracy validated at ≤1.5% coefficient of variation (CV) for 100 µL transfers across all 96 wells—measured per ISO 8655-6 guidelines using gravimetric verification on certified analytical balances.
• Adjustable dispensing speed with two preset modes (“High” and “Low”) to optimize flow dynamics for low-volatility buffers or shear-sensitive biomolecules.
• Integrated auto-cleaning protocol with programmable pre-wet, rinse, and aspirate-retract cycles—each step timed and volume-controlled to minimize residual carryover and support multi-reagent workflows.
• 7-inch capacitive-touch TFT display with intuitive icon-driven interface; supports method storage, user-level access control (operator/admin), and real-time status feedback including tubing wear indicators.
• Modular waste management: removable polypropylene waste tray with optional sterile tubing connection to centralized 2-L HDPE waste reservoir—compatible with vacuum-assisted evacuation systems.

Sample Compatibility & Compliance

The CYFY-8 accommodates standard ANSI/SBS-compliant 96-well plates (flat, U-bottom, V-bottom, and PCR-ready formats), as well as shallow-well assay plates with ≥1 mm rim height. Fluid path materials—including platinum-cured silicone tubing, PTFE-coated fittings, and chemically inert polypropylene manifolds—are certified for repeated exposure to 30% v/v ethanol, glacial acetic acid, 4 M NaCl, 1 M H₃PO₄, and 0.5 M NaOH. No metal components contact liquid streams, eliminating corrosion risk and ensuring long-term calibration stability. While not FDA 510(k)-cleared, the instrument’s mechanical design, firmware logging capabilities (timestamped method execution and cleaning records), and physical separation of reagent lines align with GLP and GMP Annex 11 expectations for automated equipment used in regulated QC environments.

Software & Data Management

The embedded firmware supports method export/import via USB flash drive in CSV format, preserving all dispensing parameters (volume, speed mode, columns selected, wash cycles). Audit trails—including operator ID, start/stop timestamps, error codes, and executed cleaning logs—are stored internally for ≥30 days and exportable for LIMS integration. Optional PC-based configuration software (Windows 10/11 compatible) enables advanced scheduling, plate mapping visualization, and statistical process control (SPC) chart generation for routine performance verification. All data outputs comply with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) when archived externally.

Applications

• ELISA and immunoassay plate setup: uniform coating buffer, detection antibody, and substrate dispensing with minimized edge effects.
• Microbial susceptibility testing: precise inoculum delivery into antimicrobial gradient plates per CLSI M07-A10 protocols.
• Cell-based assay preparation: serum-free media supplementation, inhibitor titration series, and transfection reagent distribution.
• QC/QA sample aliquoting: stable reference material partitioning into cryovials or secondary plates under ISO/IEC 17025-controlled conditions.
• Food safety screening: rapid transfer of extraction supernatants into toxin detection microarrays or lateral flow cartridges.

FAQ

What tubing lifetime can be expected under continuous operation?
With daily use (≤8 hours, 2 reagent changes), platinum-cured silicone tubing maintains ≤2.0% volume drift over 3 months—verified via weekly gravimetric checks. Replacement kits include calibration certificates.
Is remote monitoring or network connectivity supported?
The base model operates standalone; Ethernet or Wi-Fi modules are available as factory-installed options for SNMP-based status polling and syslog forwarding.
Can the CYFY-8 dispense into non-96-well formats (e.g., 384-well or tubes)?
No—it is mechanically constrained to ANSI/SBS 96-well plate footprints. Adapters for deep-well or skirted plates require custom mounting fixtures (not included).
How is cross-contamination prevented during multi-reagent runs?
Each channel uses dedicated tubing; automated pre-wet and post-dispense aspiration-retract cycles remove residual film; optional UV-C irradiation module (add-on) treats exposed tubing surfaces between runs.
Does the system meet any international calibration standards?
Yes—gravimetric verification procedures follow ISO 8655-6 (volumetric dispensers) and USP <1251> (instrument qualification), with traceability to NIST-traceable mass standards.