Kezhe GoodLook-1000 Automated Thin-Layer Chromatography Imaging System
| Brand | Kezhe / KEZHE SHANGHAI |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Product Category | Domestic |
| Model | GoodLook-1000 |
| Instrument Type | TLC Imaging System |
| Measurement Modes | Reflectance Absorption, Reflectance Fluorescence, Transmittance Absorption, Transmittance Fluorescence |
| Wavelength Range | 190–800 nm |
| Light Sources | Xenon lamp, Tungsten-halogen lamp, High-pressure Mercury lamp |
| Monochromator | Holographic grating (1200 lines/mm) with nitrogen purge capability |
| Spectral Bandwidth | 5 nm |
| Wavelength Accuracy | < 1 nm |
| Wavelength Repeatability | < 0.2 nm |
| Minimum Spatial Resolution | 25 µm |
| Imaging Wavelengths | 254 nm, 365 nm, White light (400–700 nm) |
| Sample Stage Capacity | Accommodates 200 mm × 200 mm TLC plates |
| Maximum Scanning Speed | 100 mm/s |
| Camera Resolution | 12 MP |
| Exposure Time Range | 0–10 s |
| Gain Adjustment | 0–100% |
| Imaging Modalities | Visible, UV, Fluorescence, Chemiluminescence |
Overview
The Kezhe GoodLook-1000 Automated Thin-Layer Chromatography (TLC) Imaging System is a purpose-built digital imaging platform engineered for high-fidelity acquisition, quantitative analysis, and regulatory-compliant documentation of thin-layer chromatograms. Designed in alignment with pharmacopoeial requirements—including the Chinese Pharmacopoeia (2010 and 2015 editions)—the system implements calibrated reflectance and transmittance optical detection across ultraviolet (254 nm and 365 nm), visible (400–700 nm), and fluorescence modalities. Its optical architecture integrates a low-stray-light imaging chamber, nitrogen-purged monochromator with 1200-line/mm holographic grating, and precision-controlled multi-source illumination to ensure spectral fidelity and photometric stability. The system operates on a fixed-focus industrial lens assembly and a 12-megapixel scientific-grade camera, delivering spatial resolution down to 25 µm—sufficient to resolve closely migrating bands on standard silica or polyamide TLC plates. Unlike manual visual inspection or generic document scanners, the GoodLook-1000 captures digitally traceable, intensity-calibrated images suitable for quantitative densitometry and multivariate pattern recognition.
Key Features
- Fully computer-controlled operation—no manual switches or hardware buttons; all illumination, exposure, focus, and scanning parameters are software-defined and reproducible.
- Dual-wavelength UV excitation (254 nm and 365 nm) with independently adjustable gain and exposure time (0–10 s), enabling optimized signal-to-noise ratio for both quenching and fluorescence-based detection.
- Nitrogen-purged monochromator ensures long-term wavelength stability (<0.2 nm repeatability) and minimizes ozone generation during deep-UV operation.
- Integrated audit trail functionality compliant with FDA 21 CFR Part 11: records user identity, timestamp, parameter changes, image processing steps, and report generation events.
- Three-tier user permission model with mandatory password rotation every 90 days and electronic signature support for GxP-compliant reporting.
- Advanced image correction suite: geometric distortion correction, background subtraction, dual-window comparative overlay, saturation/contrast normalization, and frame-to-frame registration for multi-exposure fusion.
- Automated Rf calculation, peak height/area integration, and similarity clustering algorithms embedded in the dedicated Traditional Chinese Medicine (TCM) Fingerprint Analysis Module.
Sample Compatibility & Compliance
The GoodLook-1000 accommodates standard and custom TLC plates up to 200 mm × 200 mm, including silica gel 60 F254, aluminum-backed cellulose, RP-18, and chiral stationary phases. Its low-stray-light dark chamber and high-sensitivity camera enable reliable detection of weakly fluorescent or low-absorbance analytes—critical for herbal extract profiling and impurity tracking. The system conforms to GLP and GMP operational frameworks: data integrity is enforced via encrypted database backups, immutable audit logs, role-based access control, and electronic signatures meeting ISO/IEC 17025 and WHO TRS 961 Annex 5 expectations. All image metadata—including wavelength, exposure time, gain, lamp hours, and calibration date—is embedded in TIFF and PDF/A export formats for long-term archival and regulatory submission.
Software & Data Management
The bundled Kezhe TLC Image Suite comprises four interoperable modules: (1) Hardware Control Engine for synchronized lamp activation, stage positioning, and camera triggering; (2) Image Processing Core supporting gamma correction, deconvolution-based sharpening, and spectral unmixing for multi-channel overlays; (3) TCM Fingerprint Expert System implementing hierarchical clustering (HCA), principal component analysis (PCA), and cosine similarity scoring per ICH Q5C guidelines; and (4) Compliance Manager enforcing 21 CFR Part 11 Annex 11 requirements—e.g., operator authentication, change history logging, and electronic record retention. Databases are stored in AES-256 encrypted SQLite format with automated daily backup and point-in-time restoration. Export options include CSV (for LIMS integration), XML (for CDISC SDTM), and PDF/A-2b (for regulatory dossiers).
Applications
- Standardized documentation and batch-to-batch comparison of TLC plates in TCM manufacturing QA/QC laboratories.
- Generation of high-resolution chromatographic images for new drug application (NDA) submissions under NMPA and EMA requirements.
- Development and validation of fingerprint chromatographic methods for multi-component herbal formulations per Chinese Pharmacopoeia General Chapter 9101.
- Quantitative assessment of marker compound ratios in blended extracts to ensure batch consistency and dosage uniformity.
- Stability-indicating analysis—tracking degradation product formation under accelerated stress conditions using dual-wavelength densitometry.
- Academic research in natural product chemistry, including isolation pathway verification and chemotaxonomic profiling.
FAQ
Does the GoodLook-1000 support method validation per ICH Q2(R2)?
Yes—the system provides full traceability of acquisition parameters, image processing steps, and quantitative outputs required for analytical procedure validation. Calibration reports, system suitability test (SST) templates, and linearity/range documentation tools are included.
Can raw image data be exported for third-party analysis?
Yes—TIFF files retain full 16-bit dynamic range and embedded EXIF metadata. Processed data (Rf, peak area, similarity scores) export to CSV, Excel, or XML with configurable field mapping.
Is nitrogen purge mandatory for routine operation?
Nitrogen purging is optional but recommended for extended 254 nm operation to suppress ozone accumulation and maintain optical path clarity—particularly during high-throughput fingerprinting workflows.
How is photometric accuracy maintained over time?
The system includes built-in reference standards (neutral density filters and fluorescent intensity tiles) for periodic verification. Software-guided recalibration routines log drift trends and trigger maintenance alerts when deviations exceed ±2% of baseline response.
What regulatory documentation is supplied with the instrument?
Each unit ships with Factory Acceptance Test (FAT) report, IQ/OQ protocols aligned with ASTM E2500-13, 21 CFR Part 11 compliance statement, and a complete electronic validation toolkit (including test scripts and expected result databases).
