Jialemei BXD-Pro Portable Nucleic Acid Rapid Detection System for Bursaphelenchus xylophilus
| Brand | Jialemei |
|---|---|
| Origin | Sichuan, China |
| Manufacturer Type | Manufacturer |
| Product Origin | Domestic (China) |
| Model | BXD-Pro |
| Pricing | Upon Request |
Overview
The Jialemei BXD-Pro Portable Nucleic Acid Rapid Detection System is a field-deployable isothermal amplification platform engineered for the rapid, on-site molecular identification of Bursaphelenchus xylophilus, the causal agent of pine wilt disease. Unlike conventional PCR-based systems requiring thermocycling and centralized laboratory infrastructure, the BXD-Pro leverages an enzyme-characterization-driven molecular enzymatic cycling amplification (MECA) technology—distinct from LAMP or RPA—to achieve sequence-specific nucleic acid detection under constant temperature conditions (60–65 °C). This principle enables robust amplification directly from crude wood chip or nematode suspension samples without prior DNA extraction or purification. Designed for frontline forest health monitoring, the system delivers actionable diagnostic results within 25 minutes from sample introduction to visual or instrument-assisted readout, fulfilling urgent needs in early outbreak containment, quarantine inspection, and regulatory surveillance.
Key Features
- True Field Portability: Total system mass < 3 kg; footprint ≤ 0.1 m²; operates with external power banks (12 V DC input) enabling up to 6 hours of continuous operation—sufficient for ~200 assays per charge.
- Minimalist Workflow: Two-step protocol—sample loading followed by one-button initiation—eliminates manual pipetting, thermal ramping, or reagent aliquoting. No trained molecular biologist required.
- Direct Sample Compatibility: Validated for unprocessed pine wood chips and nematode suspensions; no DNA extraction kit or centrifugation step needed.
- High Analytical Sensitivity: Demonstrated single-nematode detection capability in independent technical validation studies; validated against reference strains and field-collected isolates.
- Standardized Output: Integrated optical reader with quantitative fluorescence interpretation (optional) and binary positive/negative result display aligned with national technical evaluation criteria (China National Science and Technology Achievement Appraisal Certificate No. 2023-XXX).
Sample Compatibility & Compliance
The BXD-Pro accepts native wood chip homogenates (≤ 50 mg) and aqueous nematode suspensions prepared via standard sieving and centrifugal flotation. It complies with Chinese Forestry Industry Standard LY/T 2948–2018 for B. xylophilus molecular detection and has undergone formal appraisal by the China National Forestry and Grassland Administration. While not certified to ISO/IEC 17025 for accredited testing laboratories, its assay performance metrics—including limit of detection (LoD), repeatability (CV < 8% across 10 replicates), and cross-reactivity screening against 12 non-target nematode species—meet internal validation requirements for official phytosanitary screening use. The system supports GLP-aligned recordkeeping when paired with optional cloud-synced reporting modules.
Software & Data Management
The embedded firmware (v2.4+) provides local assay logging including timestamp, operator ID (optional PIN entry), sample ID, and result status. Raw fluorescence kinetics and endpoint values are exportable via USB-C to CSV format. Optional Bluetooth-enabled mobile application (iOS/Android) enables remote result aggregation, geotagging, and batch-level summary reports compliant with China’s National Forest Pest Monitoring Platform data schema. Audit trails meet basic traceability requirements for internal quality review; full 21 CFR Part 11 compliance requires integration with third-party LIMS platforms.
Applications
- Rapid pre-quarantine screening of imported timber and packaging materials at ports of entry.
- Field-level surveillance in high-risk pine forest zones during peak transmission season (July–October).
- Verification of fumigation or heat-treatment efficacy in sawmills and log yards.
- Supporting epidemiological mapping in collaboration with provincial forestry bureaus and research institutes.
- Educational deployment in forestry extension programs for technician-level capacity building.
FAQ
Does the BXD-Pro require cold-chain reagents or refrigerated storage?
No. All lyophilized master mix components are stable at ambient temperature (15–30 °C) for ≥12 months; no freezer or ice packs are required.
Can the system detect other pine pathogens such as Fusarium spp. or Ophiostoma spp.?
Not natively. The current assay cartridge targets a highly conserved region of the B. xylophilus 18S rRNA gene. Multiplex cartridge development is underway but not commercially available.
Is training provided for field operators?
Yes. Jialemei offers standardized 4-hour on-site or virtual operator certification covering safety, troubleshooting, and documentation protocols.
What maintenance is required?
Annual calibration verification using supplied control cartridges; optical path cleaning every 50 runs with lint-free swabs and 70% ethanol.
Is regulatory approval documentation available for international import?
CE marking is not held; export to EU, US, or Japan requires end-user-initiated country-specific registration under applicable biotechnology or plant health regulations.
