MEMMERT HPP110 LED Illuminated Climate Chamber

Overview

The MEMMERT HPP110 LED Illuminated Climate Chamber is a precision-engineered upright environmental test chamber designed for long-term, reproducible simulation of complex diurnal cycles under controlled temperature, relative humidity, and spectrally tunable visible light. Unlike conventional compressor-based chambers, the HPP110 employs solid-state thermoelectric (Peltier) cooling combined with high-efficiency LED illumination—eliminating mechanical vibration, refrigerant use, and excessive thermal load. Its measurement architecture is built around dual-redundant, traceable sensing: a four-wire PT100 Class A temperature probe compliant with DIN EN 60751 and a capacitive humidity sensor calibrated across the full operational range. The chamber meets DIN 12 880:2007-05 for temperature stability (≤ ±0.1 °C drift over time) and uniformity (≤ ±0.4 °C across working volume), making it suitable for GLP-compliant biological incubation, seed germination studies, entomological rearing (e.g., Drosophila melanogaster), and photobiological assays where spectral fidelity and micro-vibration suppression are critical.

Key Features

• LED illumination system with two selectable spectral modes: cool white (5500 K) and blended cool/warm white (2700 K), enabling simulation of natural daylight progression or controlled photoperiods
• 10,000 lux maximum irradiance (measured at 10 cm), adjustable in precise 10% increments—ideal for dose-response experiments requiring quantifiable photon flux
• Thermoelectric cooling eliminates compressor-induced vibration (<0.5 µm RMS), essential for sensitive developmental biology and microcosm studies
• Microprocessor-controlled humidification using distilled water recirculation and thermally isolated steam generation—prevents condensation and microbial ingress per ISO 8573-1:2010 Class 3 air purity requirements
• Triple-layer safety architecture: hardware-based overtemperature cutoff (Protection Class 3.3), Automatic Safety Function (ASF), and independent relay-driven heater disable—fully compliant with IEC 61010-1
• On-device calibration capability (3-point temperature, 2-point humidity at 20 %RH and 90 %RH) without external PC or proprietary software

Sample Compatibility & Compliance

The HPP110 accommodates standard laboratory vessels—including Petri dishes, multiwell plates, growth trays, and insect culture vials—within its 108 L stainless steel chamber. Its low-vibration operation ensures undisturbed development in embryonic, larval, and pupal stages of model organisms. The chamber supports regulatory workflows aligned with ISO 17025 (for accredited labs), USP (environmental control for pharmaceutical stability testing), and OECD Test Guidelines 202/211 (aquatic and terrestrial ecotoxicology). All firmware and data logging functions comply with ALCOA+ principles: attributable, legible, contemporaneous, original, accurate, complete, consistent, enduring, and available—enabling audit readiness for FDA 21 CFR Part 11–governed environments when paired with validated third-party software.

Software & Data Management

Internal logging stores temperature, relative humidity, illumination status, setpoints, errors, and timestamps in non-volatile memory (1024 KB), supporting up to three months of 1-minute-interval records. Data export is supported via parallel port to PCL3-compatible printers; USB connectivity is achievable using an IEEE 1284-to-USB converter. MEMMERT’s optional MEMoryCard XL enables advanced programming: up to 40-step weekly schedules with independent T/RH/light profiles per stage, including weekend-only or weekday-only group modes. All logged data includes digital signatures for integrity verification. Firmware updates are performed via SD card—no network exposure required—ensuring cybersecurity compliance in isolated lab networks.

Applications

• Plant physiology research: photoperiod-controlled germination, photomorphogenesis, and stress response under defined spectral quality
• Entomology: low-disturbance rearing of Drosophila, Tribolium, and parasitoid wasps where mechanical vibration disrupts mating or oviposition behavior
• Pharmaceutical stability testing: ICH Q1A(R2)-aligned long-term and accelerated storage conditions with integrated light exposure per Q5C guidelines
• Microbial ecology: controlled biofilm formation and fungal sporulation under synchronized diurnal cycles
• Material science: humidity-coupled aging of hygroscopic polymers and optical coatings under non-condensing RH gradients

FAQ

What is the maximum illumination intensity, and how is it measured?
Maximum irradiance is 10,000 lux, measured at a perpendicular distance of 10 cm from the LED array using a calibrated lux meter traceable to NIST standards.
Can the HPP110 operate continuously at 40 °C with 85 %RH and full illumination?
Yes—the thermoelectric cooling system and recirculating humidification are engineered for sustained operation across the full specified envelope, including simultaneous maxima, as verified per DIN 12 880 thermal load testing protocols.
Is the data logger compliant with 21 CFR Part 11?
The embedded logger satisfies core ALCOA+ attributes; full Part 11 compliance requires integration with MEMMERT’s validated MEMcontrol software suite and institutional electronic signature infrastructure.
How does the chamber prevent condensation during rapid humidity transitions?
The microprocessor dynamically modulates heating power and humidifier duty cycle while monitoring dew point in real time—activating anti-condensation pre-heating before RH ramp-up, per ISO 8573-1 moisture control logic.
Are calibration certificates included with shipment?
A factory-issued calibration report (traceable to DAkkS-accredited standards) is provided; on-site user calibration is supported via front-panel interface without external tools or software.