HengAo HSE-24D Circular Manual Solid Phase Extraction (SPE) System

Overview

The HengAo HSE-24D Circular Manual Solid Phase Extraction (SPE) System is a robust, benchtop sample preparation platform engineered for reproducible, high-throughput isolation and concentration of target analytes from complex liquid matrices. Based on classical vacuum-assisted cartridge-based SPE methodology, the system applies controlled negative pressure across conditioned sorbent cartridges to achieve selective retention, washing, and elution of analytes—enabling efficient removal of interfering matrix components while preserving analyte integrity and recovery. Its circular manifold architecture ensures uniform vacuum distribution across all 24 channels, minimizing inter-channel flow variability and enhancing method reproducibility. Designed for laboratories requiring rigorous compliance with standardized sample preparation protocols—including those aligned with EPA Methods 500/525, ISO 17993, and AOAC Official Methods—the HSE-24D supports GLP/GMP-aligned workflows through consistent mechanical design and chemically inert fluidic pathways.

Key Features

• 24-position circular vacuum manifold with precision-machined PTFE-sealed ports and integrated pressure regulation for uniform flow control across all channels
• Manually adjustable vacuum source interface compatible with standard lab vacuum pumps or regulated nitrogen manifolds (0–760 torr range)
• Dual-layer vacuum chamber design featuring an upper gas dispersion chamber and lower solvent collection reservoir—preventing aerosol carryover and cross-contamination during high-flow elution
• All wetted components constructed from high-purity PTFE, 316 stainless steel, or borosilicate glass—ensuring compatibility with aggressive solvents (e.g., acetonitrile, methanol, dichloromethane, 1% formic acid) and resistance to corrosion over extended use
• Modular column adapter system supporting standard 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges—including stacked-bed and mixed-mode formats
• Integrated drip tray and removable waste bottle (1 L capacity) with level indicator and overflow protection
• Ergonomic height-adjustable support frame accommodating varied collection vessel sizes—from microcentrifuge tubes to 100 mL volumetric flasks

Sample Compatibility & Compliance

The HSE-24D accommodates aqueous, semi-aqueous, and organic liquid samples—including environmental water extracts, food homogenates, biological fluids (urine, plasma, serum), beverage filtrates, and hydrolyzed tissue digests. It supports both reversed-phase (C18, C8), ion-exchange (SCX, SAX), and mixed-mode sorbents for broad-spectrum analyte capture. The system meets material compatibility requirements outlined in USP , ASTM D5116 (for VOC analysis), and ISO/IEC 17025 clause 5.4.2 regarding equipment suitability for intended analytical procedures. Its all-PTFE sealing surfaces and absence of elastomeric gaskets eliminate leachable organics—critical for low-level pesticide residue, pharmaceutical impurity, and endocrine disruptor analyses compliant with EU SANTE/11312/2021 guidelines.

Software & Data Management

As a manually operated platform, the HSE-24D does not incorporate embedded software or digital controls. However, its mechanical design supports full integration into documented laboratory workflows governed by FDA 21 CFR Part 11-compliant electronic lab notebooks (ELNs) and LIMS systems. Users may log operational parameters—including vacuum setpoint, conditioning/wash/elution volumes, solvent identities, and batch identifiers—via structured SOP templates. Traceability is maintained through physical record linkage to associated analytical runs (e.g., LC-MS/MS, GC-MS). Optional barcode-labeled cartridge trays and pre-printed SPE log sheets facilitate audit-ready documentation for ISO 17025 accreditation assessments.

Applications

• Environmental analysis: Extraction of trace-level organochlorine pesticides, PAHs, phthalates, and heavy metal chelates from surface water, wastewater, and soil leachates
• Food safety testing: Multi-residue analysis of veterinary drug residues (e.g., fluoroquinolones, sulfonamides), mycotoxins (aflatoxin B1, ochratoxin A), and process contaminants (3-MCPD, furan) in milk, honey, cereals, and vegetable oils
• Clinical and forensic toxicology: Isolation of opioids, benzodiazepines, amphetamines, and novel psychoactive substances from whole blood, oral fluid, and hair digests
• Pharmaceutical quality control: Purification of active pharmaceutical ingredients (APIs) and degradation products from stability study samples prior to HPLC-UV quantification
• Beverage authenticity screening: Removal of pigments, tannins, and polysaccharides from fruit juice and wine matrices to improve GC-FID detection of adulterants (e.g., synthetic sweeteners, preservatives)

FAQ

Is the HSE-24D compatible with automated fraction collectors or robotic liquid handlers?
Yes—its standardized 24-position layout and modular column adapters allow seamless integration with third-party autosamplers and fraction collectors equipped with SPE-compatible gripper arms and rack positioning systems.
Can it be used for offline two-dimensional SPE (2D-SPE)?
Yes—the system supports tandem cartridge configurations (e.g., loading on a strong cation exchange column followed by transfer to a C18 trap column) using optional inter-cartridge transfer tubing kits.
What vacuum pump specifications are recommended?
A two-stage oil-free diaphragm pump delivering ≥50 L/min free air displacement and capable of sustaining ≤50 mbar absolute pressure is optimal for full 24-channel operation at 10–20 mL/min flow rates.
Does the system include cartridge conditioning manifolds or solvent reservoirs?
No—conditioning, washing, and elution solvents must be delivered manually via glass or PTFE syringes or gravity-fed reservoirs; however, standardized solvent reservoir mounts are available as accessories.
How is method validation supported?
The system’s geometric consistency, material inertness, and channel-to-channel flow equivalence enable straightforward assessment of recovery (%R), precision (RSD <5%), and breakthrough volume—key parameters required under ICH Q2(R2) and SANTE/11312/2021 validation frameworks.