INNOTEG EasySPE-E8 Semi-Automatic Solid Phase Extraction System

Overview

The INNOTEG EasySPE-E8 is a semi-automatic, 8-channel solid phase extraction (SPE) system engineered for reproducible, scalable, and method-compliant sample preparation in analytical laboratories. It operates on the fundamental principle of selective analyte retention and elution using functionalized sorbent cartridges—enabling efficient cleanup, concentration, and fractionation of complex matrices prior to downstream analysis by HPLC, GC, LC-MS, or ICP-MS. Unlike fully automated platforms, the EasySPE-E8 retains operator control over critical timing and solvent selection while eliminating manual syringe-driven variability through precision peristaltic pumping. Each channel features independent flow calibration and real-time pressure monitoring, ensuring uniform breakthrough behavior across all eight positions—even during high-volume aqueous extractions (up to 10 L). The system’s PTFE-lined fluid path guarantees chemical inertness with aggressive solvents (e.g., dichloromethane, acetonitrile, methanol, and 0.1 M NaOH), supporting both reversed-phase and ion-exchange chemistries without leaching or degradation.

Key Features

• Eight parallel, independently controlled peristaltic pump channels with individual calibration routines—minimizing inter-channel flow deviation and enhancing method reproducibility.
• PTFE (Teflon®)-lined tubing and manifold components ensure compatibility with organic solvents, strong acids, and bases—critical for robust method transfer and long-term system durability.
• Integrated reservoir-based loading architecture: large-capacity sample and solvent reservoirs allow unattended processing of up to 10 L aqueous samples without manual refilling.
• Touchscreen HMI with intuitive workflow-guided software enables step-by-step execution of conditioning, loading, washing, and elution—each stage programmable with custom volume, flow rate, and dwell time.
• Dynamic channel balancing: micro-adjustable flow resistance per channel compensates for cartridge bed density variation, preventing premature channel clogging or uneven elution profiles.
• Modular expansion support: optional tandem dual-cartridge configuration enables sequential mixed-mode extraction (e.g., C18 + SCX), while 47 mm disk adapter kits extend applicability to membrane-based SPE protocols.

Sample Compatibility & Compliance

The EasySPE-E8 accommodates a broad spectrum of biological, environmental, and food matrices—including plasma, urine, drinking water, wastewater, fruit juice, milk, and soil extracts. Its validated performance aligns with widely adopted regulatory frameworks: EPA Method 525.3 (drinking water), EPA Method 1694 (pharmaceuticals in wastewater), ISO 17025-accredited workflows, and AOAC Official Methods for pesticide residue analysis. All firmware and software logs maintain timestamped audit trails compliant with GLP and GMP documentation requirements. While not FDA 21 CFR Part 11–certified out-of-the-box, the system supports integration with LIMS and electronic lab notebook (ELN) platforms via CSV export and USB data dump—facilitating traceable data archival and review.

Software & Data Management

The embedded control interface provides guided SOP execution with parameter locking, user-level access control (admin/operator modes), and real-time status visualization (flow rate, elapsed time, remaining volume). All method parameters—including pump speed (rpm), reservoir fill levels, and step durations—are saved as reusable templates. Raw operation logs are stored internally and exportable in .csv format for QC review or external statistical process control (SPC) analysis. No cloud connectivity or remote telemetry is implemented—ensuring full data sovereignty and alignment with institutional IT security policies common in government and academic labs.

Applications

• Multi-residue pesticide and veterinary drug analysis in food commodities (e.g., fruits, vegetables, meat, honey) per EU SANTE/11312/2021 guidelines.
• Trace-level detection of pharmaceuticals, endocrine disruptors, and PFAS in surface water, groundwater, and drinking water—particularly under large-volume enrichment protocols (≥1 L).
• Forensic toxicology workflows: isolation of amphetamines, opioids, and benzodiazepines from whole blood and urine with minimized matrix interference.
• Environmental monitoring of PAHs, PCBs, and organochlorine pesticides in sediment porewater and wastewater effluents.
• Method development and validation studies requiring rigorous inter-day and inter-operator repeatability assessment.

FAQ

What types of SPE cartridges are compatible with the EasySPE-E8?
Standard 1 mL, 3 mL, 6 mL, and 12 mL polypropylene or glass SPE cartridges are supported. Optional 47 mm disk holders accommodate bonded-phase membranes for high-flow applications.
Can the system handle viscous or particulate-laden samples?
Pre-filtration (e.g., 0.45 µm PVDF syringe filters) is recommended for samples containing suspended solids. For viscous matrices like serum or honey, dilution or solvent-assisted homogenization prior to loading is advised to prevent channel blockage.
Is method validation documentation available?
Yes—INNOTEG provides a basic IQ/OQ protocol template and instrument-specific performance verification data (flow accuracy, channel-to-channel CV%, carryover test results) upon request.
Does the system support gradient elution or multi-solvent switching?
No—the EasySPE-E8 uses discrete solvent reservoirs per step; gradient programming is not implemented. However, up to four distinct solvents can be pre-loaded into separate reservoirs and selected manually or via sequential step commands.
What maintenance is required for long-term reliability?
Routine replacement of peristaltic pump tubing every 3–6 months (depending on usage intensity) and quarterly calibration verification using gravimetric flow checks are recommended. PTFE tubing does not require lubrication or cleaning between runs.